Universal genomic dna kit
The Universal Genomic DNA Kit is a laboratory product designed for the extraction and purification of genomic DNA from a variety of sample types. The kit provides a standardized protocol and reagents to facilitate the DNA isolation process.
Lab products found in correlation
50 protocols using universal genomic dna kit
Genome Editing Validation by T7 Endonuclease I
CRISPR Gene Editing Validation Protocol
Mitogenome Sequencing of Dolycoris Specimens
Quantification of Mitochondrial DNA
Genetic basis of H. pylori antibiotic resistance
Assessing Off-Target Effects of Nanocapsules
Briefly, after being treated with ANCSS(Cas9/sgPLK1), the genomic DNAs of tumor, normal brain tissue, liver, and kidney were harvested from mice bearing U87MG or CSC2 GSCs, using the Universal Genomic DNA Kit (CWBIO, China) according to the manufacturer’s instructions. One hundred nanograms of genomic DNA was used as a template to perform PCR using primers designed against on-target and off-target sites. Purified DNA was amplified again by PCR with primers containing sequencing adapters and then sequenced and analyzed by Sangon Biotechnology Company (Shanghai, China) to detect indels around target sites.
16S rRNA Gene Amplification and Sequencing
Sampling and DNA Extraction of Eurasian Apaludum
CRISPR-Cas9 Modification Detection
Comprehensive Genomic DNA and RNA Isolation Protocols
Total RNA was isolated from cells using the RNApure Tissue & Cell Kit (Cwbiotech). Isolated RNA was used as a template for reverse transcription reactions using a HiFiScript cDNA Synthesis Kit (Cwbiotech).
Quantitative real-time PCR analyses were performed with a PCR mixture containing 1 μmol/L of each primer and SYBR Green Real-Time PCR Master Mixes (Thermo Fisher) using a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). Each sample was run in triplicate, and the mRNA level of GAPHD was used as an internal control. The cycle threshold (Ct) value was normalized to the GAPDH level using the 2−△△Ct method. The primers are listed in Supplementary Table
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