Elisa

Manufactured by Phoenix Pharmaceuticals

Sourced in United States

ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory technique used to detect and quantify specific substances, typically proteins, in a liquid sample. It utilizes the reaction between an antibody and an antigen to measure the concentration of the target molecule. ELISA is a versatile tool that can be applied in various fields, including clinical diagnostics, research, and environmental testing.

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Lab products found in correlation

Xmark microplate spectrophotometer, by Bio-Rad (4 mentions) Elisa, by Merck Group (2 mentions) Automated analyzer, by Abbott (1 mentions) Elisa, by Cusabio (1 mentions) Radioimmunoassay, by Merck Group (1 mentions) Elisa kit, by Cayman Chemical (1 mentions) Elisa, by R&D Systems (1 mentions) 10 kda spin column, by Abcam (1 mentions) Synergy ht multi detection microplate reader, by Agilent Technologies (1 mentions) Alphalisa technology, by PerkinElmer (1 mentions) Optiplate microplates, by PerkinElmer (1 mentions) Alphalisa, by PerkinElmer (1 mentions) Envision multilabel reader, by PerkinElmer (1 mentions) V plex assay, by Mesoscale (1 mentions) Enzyme immunoassay, by Tecan (1 mentions) Ek 049 03, by Phoenix Pharmaceuticals (1 mentions) Ek 059 02, by Phoenix Pharmaceuticals (1 mentions) Variant 2, by Bio-Rad (1 mentions) Model hem 752 fuzzy, by Omron (1 mentions) Dpp4 010, by Merck Group (1 mentions)

35 protocols using elisa

Quantifying Irisin Levels in Mice

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Irisin in mouse serum was measured using commercially-available ELISAs (Phoenix Pharmaceuticals, Burlingame, CA, USA).

Jang Y.J., Son H.J., Choi Y.M., Ahn J., Jung C.H, & Ha T.Y. (2017). Apigenin enhances skeletal muscle hypertrophy and myoblast differentiation by regulating Prmt7. Oncotarget, 8(45), 78300-78311.

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Metabolic Profiling in Prader-Willi Syndrome

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The study protocol was approved by the Institutional Review Board of Shaare Zedek Medical Center. Written informed consent was obtained from each of the participants. Subjects with genetically confirmed PWS were recruited from the Israeli National Multidisciplinary PWS Clinic at Shaare Zedek Medical Center, Jerusalem. The control group was comprised of volunteers: 9 with body mass index (BMI) <25 kg/m² (6 males/3 females) and 24 overweight or obese (11 males/13 females) with BMI >25 kg/m². Subjects with chronic illnesses, uncontrolled hypothyroidism, diabetes mellitus, or exposure to hormone medication including estrogen-containing compounds, glucocorticoids, or androgens were excluded. Venous whole blood was collected from subjects, separated to plasma, and stored at −80 °C until further assessed. The Israeli participants underwent biochemical testing, following a 12 h fast for serum lipid profile, glucose and insulin. Measurements of leptin and adiponectin were performed by ELISAs (Phoenix, Pharmaceuticals, USA). The homeostasis model assessment insulin resistance (HOMA-IR) was calculated as fasting serum insulin ([μU/mL] X fasting plasma glucose [mmol/L]/22.5). Height and weight were measured and the BMI was calculated.

Knani I., Earley B.J., Udi S., Nemirovski A., Hadar R., Gammal A., Cinar R., Hirsch H.J., Pollak Y., Gross I., Eldar-Geva T., Reyes-Capo D.P., Han J.C., Haqq A.M., Gross-Tsur V., Wevrick R, & Tam J. (2016). Targeting the endocannabinoid/CB1 receptor system for treating obesity in Prader–Willi syndrome. Molecular Metabolism, 5(12), 1187-1199.

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Adropin Quantification in Plasma and Tissue

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Adropin concentrations in plasma and aortic tissues were measured using an ELISA (#032–35, Phoenix Pharmaceuticals, Burlingame, CA, USA). All samples were assayed in duplicate. Optical densities at 450 nm were measured using an xMark microplate spectrophotometer (xMark microplate spectrophotometer; Bio‐Rad Laboratories, Hercules, CA, USA). Readings were converted to concentrations by linear fitting to a log–log plot of the standard curve.

Fujie S., Hasegawa N., Horii N., Uchida M., Sanada K., Hamaoka T., Padilla J., Martinez‐Lemus L.A., Maeda S, & Iemitsu M. (2021). Aerobic Exercise Restores Aging‐Associated Reductions in Arterial Adropin Levels and Improves Adropin‐Induced Nitric Oxide‐Dependent Vasorelaxation. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(10), e020641.

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Serum Adropin Quantification by ELISA

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Serum adropin levels were measured in duplicate using an enzyme‐linked immunosorbent assay (ELISA; Phoenix Pharmaceuticals, Burlingame, CA, USA). Optical densities at 450 nm were measured using an xMark microplate reader (xMark microplate spectrophotometer; Bio‐Rad Laboratories, Hercules, CA, USA). All samples were converted into concentrations by linear fitting to the log–log plot of a standard curve. The day‐to‐day coefficient of variation of serum adropin levels was 2.8±0.7 %.

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Metabolic and Hormonal Profiling

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Waist circumference and hip circumference were measured for calculation of the WHR. The HOMA-IR was calculated using the following equations [41 (link)]: HOMA-IR = FIns (μU/mL) × fasting blood glucose (FBG) (mmol/L)/22.5. Insulin was measured by ELISA. Free fatty acid (FFA) was measured with a commercial kit. TC, high-density lipoprotein cholesterol (HDL-C), LDL-C, and TG were analyzed using an autoanalyzer. Serum sex hormone including LH, follicle- stimulating sormone (FSH), TEST and progestogen (Prog), prolactin (PRL) and estradiol (E₂) were measured with electrochemi-luminescence immunoassay (Roche Diagnostics GmbH). Dehydroepiandrosteronesulfate (DHEA-S) and SHBG were performed using an automated analyzer (Abbott Laboratories, Abbott Park, IL). FAI was calculated as (testosterone/SHBG) × 100. Circulating betatrophin concentration was determined with an ELISA (Phoenix Pharmaceuticals Inc. Belmont, CA, USA) by using the manufacturer’s protocol.

Wang H., Du L., Wu T., Yang G., Hu W., Wang H., Yang M., Liu D., Gu H.F., Zhu Z., Zheng H, & Li L. (2017). Circulating betatrophin is associated with insulin resistance in humans: cross-sectional and interventional studies in vivo and in vitro. Oncotarget, 8(57), 96604-96614.

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Plasma Cortisol and Ghrelin Analysis

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Venous blood samples were taken from all of the participants to determine plasma cortisol and ghrelin levels. The blood samples were centrifuged at 3000 rotations per minute at 4 °C for 10 min. The plasma samples were stored at − 80 °C until analysis. Plasma cortisol levels were measured using an enzyme-linked immunosorbent assay (ELISA; Cusabio Biotech, Hubei, China; catalog no. CSB-E05111h). Plasma ghrelin levels were measured using ELISA (Phoenix Pharmaceuticals, Belmont, CA, USA; catalog no. EK-031-30). The intra- and inter-assay coefficients of variation were < 8 and 10% for cortisol and < 10 and 15% for ghrelin.

Shi L., Deng J., Chen S., Que J., Sun Y., Wang Z., Guo X., Han Y., Zhou Y., Zhang X., Xie W., Lin X., Shi J, & Lu L. (2018). Fasting enhances extinction retention and prevents the return of fear in humans. Translational Psychiatry, 8, 214.

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Plasma Apelin Quantification by ELISA

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Apelin concentrations in the plasma were measured by an enzyme-linked immunosorbent assay (ELISA; Phoenix Pharmaceuticals inc., Burlingame, CA, USA), according to the manufacturer's protocol. Apelin-12, which was used in the present study, has cross-reactivity with apelin-13 and apelin- 36. All samples were assayed in duplicate. Optical density at 450 nm was qualified using a microplate reader (xMark microplate spectrophotometer; Bio-Rad Laboratories). Samples were converted into concentration by a linear fit of the log–log plot of the standard curve.

Fujie S., Sato K., Miyamoto-Mikami E., Hasegawa N., Fujita S., Sanada K., Hamaoka T, & Iemitsu M. (2014). Reduction of Arterial Stiffness by Exercise Training Is Associated with Increasing Plasma Apelin Level in Middle-Aged and Older Adults. PLoS ONE, 9(4), e93545.

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Metabolic Biomarker Quantification Protocol

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Glucose, triglycerides, cholesterol, apolipoproteins, insulin and C-peptide were measured using standard clinical analyzer methods on an ADVIA Centaur and Roche Modular system. Total GLP-1 was measured by radioimmunoassay (Millipore [Billerica, MA]; within CV = 22%, between CV = 23% at 20pM), active GLP-1 by Meso Scale Electrochemiluminescence ([Rockville, MD]; within CV = 9.3%, between CV = 3.6% at 0.7 and 1.3 pM respectively), and pancreatic peptide YY (PYY) by ELISA (Millipore [Billerica, MA]; within CV = 2.66%, between CV =6.93% at 9.7 pM). For active GLP-1, values at the limit of detection were assigned a value of 0.65, the lowest standard in the assay. Measures made on samples from the 5 subjects in the pilot effort on pudding volume were done in the Considine lab at Indiana University. Glucose was measured using a Randox Daytona Clinical Analyzer, with insulin (within CV = 3.1%, between CV = 6.0% at 8.0 μU/ml) and c-peptide (within CV = 3.4%, between CV = 9.3% at 0.4 ng/ml) measured by radioimmunoassay (Millipore; Billerica, MA). Betatrophin was measured by ELISA (Phoenix Pharmaceuticals Inc [Burlingame, CA]; within CV = 8.9% at 1 ng/ml). HbA1c was measured by HPLC in the hospital lab as part of the subject’s pre-surgery blood work.

Shankar S.S., Mixson L.A., Chakravarthy M., Chisholm R., Acton A.J., Jones R., Mattar S.G., Miller D.L., Petry L., Beals C.R., Stoch S.A., Kelley D.E, & Considine R.V. (2017). Metabolic improvements following Roux-en-Y surgery assessed by solid meal test in subjects with short duration type 2 diabetes. BMC obesity, 4, 10.

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Biomarkers of Immune Response in Respiratory Samples

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The complete blood cell count, the plasma concentration of fibrinogen, serum C-reactive protein (CRP), and immunoglobulin E (IgE) were measured in fasting blood samples by routine laboratory techniques. Serum and plasma samples were aliquoted and stored at −70 °C until analysis. Interleukin (IL)-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, and interferon (INF)-γ in serum and BAL samples were assessed by using commercially available high-sensitivity ELISA assays (eBiosciencea, Vienna, Austria). Periostin (a renowned marker of T2-immune response) was evaluated only in BAL by ELISA (Phoenix Pharmaceuticals, Burlingame, CA, USA). Similarly, citrullinated histone 3 (H3cit), a marker of neutrophil trap formation, was measured in serum, using an ELISA kit (Cayman Chemicals, Ann Arbor, MI, USA). Most BAL cytokine measurements were below the assay threshold (results are not shown in the tables).

Bazan-Socha S., Wójcik K., Olchawa M., Sarna T., Pięta J., Jakieła B., Soja J., Okoń K., Zarychta J., Zaręba L., Stojak M., Potaczek D.P., Bazan J.G, & Celińska-Lowenhoff M. (2022). Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices. Biomedicines, 10(7), 1499.

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Canine Model of Congestive Heart Failure

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Adult mongrel dogs were anesthetized with ketamine (5.0 mg/kg IV), diazepam (0.25 mg/kg IV), and halothane (1.5% PI). A unipolar pacing lead was inserted under fluoroscopy into the right ventricular apex from the left internal jugular vein and connected to an electronic pacemaker implanted in a subcutaneous pocket in the neck. After a 24‐hour postoperative recovery period, the ventricular pacemaker was programmed to pace the ventricles at 240 bpm for 2 weeks to induce CHF as previously described.11 Atrial fibrotic remodeling is prominent in the canine CHF model produced by ventricular tachypacing, in which fibrosis plays a fundamental role in the AF substrate.2, 3 Since this model provided the initial basis for the recognition of the importance of atrial fibrosis in AF pathophysiology and has been extensively investigated in our lab,3 we used it here to address the potential role of changes in intracrine Ang‐II signaling in a clinically relevant pathological substrate. Ang‐II content was measured by ELISA (Phoenix Pharmaceuticals).

Tadevosyan A., Xiao J., Surinkaew S., Naud P., Merlen C., Harada M., Qi X., Chatenet D., Fournier A., Allen B.G, & Nattel S. (2017). Intracellular Angiotensin‐II Interacts With Nuclear Angiotensin Receptors in Cardiac Fibroblasts and Regulates RNA Synthesis, Cell Proliferation, and Collagen Secretion. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(4), e004965.

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