Topography and elemental mapping images were recorded with Ht‐7700 transmission electron microscopy (Hitachi, Japan) and high resolution TEM (HRTEM) (Tecnai G2 F20, U.S.), respectively. UV–vis spectra were captured by Lambda UV–vis spectrophotometer (Perkin Elmer, US). Dynamic light scattering (DLS) for particle size and zeta potential were determined by Zetasizer Nano ZS (Malvern, UK). The XRD patterns were tested on a TZY‐XRD (Rigaku, Japan). The experiment of Barrett‐Joyner‐Halenda model from adsorption branch of isotherms was performed with automatic surface area and porosity analyzer (Micromeritics Instrument, US). The circular dichroism (CD) spectra were measured with circular dichroism chiroptical spectrometer spectropolarimeter system (Jasco J‐1500, Japan). Oxygen (O2) generation was determined with portable dissolved oxygen meter (Rex, JPB‐607A, China). The concentration of Au was determined by inductively coupled plasma mass spectroscopy (GGL‐ICP‐MS) (PerkinElmer, US). The laser of 808 nm was administrated by power‐tunable infrared laser (Laserwave, China). Thermal images were captured by using a DT‐980 infrared camera (Huashengchang Co., Ltd., China) and analyzed by infrared image software.
Ht7700 transmission electron microscopy
Manufactured by Hitachi
Sourced in Japan
The HT7700 is a transmission electron microscope (TEM) manufactured by Hitachi. It is designed to provide high-resolution imaging and analysis of materials at the atomic scale. The HT7700 utilizes advanced electron optics and detectors to enable detailed observation and characterization of a wide range of samples, including metals, ceramics, and biological specimens.
Automatically generated - may contain errors
Lab products found in correlation
Gantan830.10w ccd camera, by Hitachi (2 mentions)
J 1500, by Jasco (1 mentions)
Zetaview s n 17 310, by Particle Metrix (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Avance 3 300 spectrometer, by Bruker (1 mentions)
Uv 2450 spectrophotometer, by Shimadzu (1 mentions)
Fl 4500 spectrofluorometer, by Hitachi (1 mentions)
214 polyma differential scanning calorimeter, by Netzsch (1 mentions)
Agilent 1260 high performance liquid chromatography, by Agilent Technologies (1 mentions)
Nanosight ns300, by Malvern Panalytical (1 mentions)
Pierce bca assay kit, by Thermo Fisher Scientific (1 mentions)
Fluorescence microscopy, by Olympus (1 mentions)
11 protocols using ht7700 transmission electron microscopy
1
Comprehensive Characterization of Nanomaterials
2
Ultrastructural Analysis of Liver Tissue
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The 1 mm × 1 mm × 1 mm trimmed liver piece was fixed in 2.5% glutaraldehyde (pH = 7.4, 4 °C) for 24 h. The samples were fixed again with OsO4 for 2 h and dehydrated with 30% (20 min), 50% (20 min), 70% (25 min), 80% (30 min), 90% (30 min), 100% I (40 min), and 100% II (40 min) acetone. The samples were soaked in epoxy resin acetone solution at 40 °C for 2 h and finally embedded in epoxy resin. Microtome was used to slice the trimmed tissue blocks to 80 nm slices. The sections were double stained with lead citrate and uranyl acetate solution for 15 min. Ultrastructural pathological damage was observed by HT7700 transmission electron microscopy (Hitachi, Japan) and photographed with GANTAN830.10W CCD camera (Hitachi, Japan).
3
Ultrastructural Analysis of Testicular Tissue
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The fresh testicular tissues were obtained and fixed at 4°C for 24 hours with 2.5% glutaraldehyde. Following to the standard procedure of TEM, the sample was coated with resin. The ultrathin sections were stained with lead citrate and uranyl acetate, and the images were captured by HT7700 transmission electron microscopy (Hitachi, Tokyo, Japan).
4
Exosome Characterization by TEM and NTA
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The pelleted exosomes were resuspended in 100 μL of PBS. The morphology and size of the isolated exosomes were observed via HT-7700 transmission electron microscopy (TEM) (Hitachi, Chiyoda-ku, Tokyo, Japan) and ZetaVIEW S/N 17–310 nanoparticle tracking analysis (NTA) (Particle Metrix, Inning am Ammersee, Munich, Germany). Specific markers for exosomes, including Alix (Cat#2171 s, CST, Danvers, MA, USA) and CD63 (Cat#ab217345, Abcam, Cambridge Biomedical Campus, Cambridge, UK), were detected by Western blot analysis as a routine procedure.
5
Characterization of Nanoparticle Synthesis
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All reagents were commercially available and used as supplied without further purification. 1H NMR spectra were recorded on a Bruker Avance III-300 spectrometer with internal standard TMS. Transmission electron microscopy (TEM) images were taken by a HT-7700 transmission electron microscopy (Hitachi, Japan). The particle size was performed by dynamic light scattering (DLS) on the Zetasizer (Nano ZS, Malvern Instruments, Worcestershire, UK).
6
Characterization of Nanomaterials
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A UV-2450 spectrophotometer (Shimadzu, Tokyo, Japan), HT-7700 transmission electron microscopy (TEM) (Hitachi, Tokyo, Japan), and a FL-4500 spectrofluorometer equipped with an Xe lamp as a excitation light source (Hitachi, Japan) were used.
7
Analytical Instrumentation for Multidisciplinary Research
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Agilent 1260 high-performance liquid chromatography (HPLC) was purchased from Agilent (California, USA), HT7700 transmission electron microscopy (TEM) was purchased from Hitachi (Tokyo, Japan), 214 polyma differential scanning calorimeter (DSC) was purchased from Netzsch (Selbe, Germany), Nicolet 380 fourier transform infrared spectroscopy was purchased from Hitachi (Tokyo, Japan), Delsa Nano size analyzer was purchased from Beckman Coulter (Kraemer Boulevard Brea, USA), ZH-YLS-1A animal locomotor actimeter was purchased from Zhenghua Biologic Apparatus facilities company (Anhui, China).
8
Characterization of Apoptotic Extracellular Vesicles
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Culture medium was substituted for α-MEM/DMEM with 500 nmol/L STS (Enzo Life Sciences) to induce apoptosis of MSCs. MSCs were labeled using the TUNEL Apoptosis Detection Kit (Applygen, C000320) and rhodamine fluorescein (red) dUTP, and apoptosis was observed using a fluorescence microscopy (Olympus). After 12 h, apoVs were extracted by differential centrifugation (Fig. 1b )39 (link). The Pierce BCA Assay Kit (Thermo Scientific) was used to quantify the apoVs concentration.
ApoVs were deposited onto a carbon-coated copper net, stained twice with 1% uranyl acetate, and their morphology was visualized using an HT7700 transmission electron microscopy (Hitachi). The size distributions were evaluated using the Nano Sight NS300 (Malvern) according to the manufacturer’s instructions. ApoV markers (CD9, CD81, CD63, Fas, calreticulin, CD44, and integrin α-5) were detected by Western blotting.
ApoVs were deposited onto a carbon-coated copper net, stained twice with 1% uranyl acetate, and their morphology was visualized using an HT7700 transmission electron microscopy (Hitachi). The size distributions were evaluated using the Nano Sight NS300 (Malvern) according to the manufacturer’s instructions. ApoV markers (CD9, CD81, CD63, Fas, calreticulin, CD44, and integrin α-5) were detected by Western blotting.
9
Ileum Ultrastructure Analysis via TEM
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Transmission electron microscopy analysis was performed according to the procedure described in the literature (Wang et al., 2018 ). The ileum pieces were carved into small pieces and immediately fixed in 2.5% glutaraldehyde (pH = 7.4, 4°C), and OsO4 was used for postfixation. After dehydrating, the samples were soaked in epoxy resin acetone solution and finally embedded in epoxy resin. Microtome was used to slice the trimmed tissue blocks into 80 nm slices. The sections were double stained with lead citrate and uranyl acetate solution. Ultrastructural architectures of the ileum were observed by HT7700 transmission electron microscopy (Hitachi, Tokyo, Japan) and photographed with GANTAN830.10 W CCD camera (Hitachi).
10
Exosome Isolation and Characterization
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Exosomes were isolated through successively centrifuging steps. Briefly, HUVECs were centrifuged at 2000 × g, 4°C for 30 min. The supernatant was transferred to a new tube and subjected to centrifugation at 10,000 ×g 4°C for 45°min to remove large vesicles. The supernatant filtered through a 0.45 μm filter was centrifuged again at 10,000×g, 4°C for 70°min. The pellets of exosomes were suspended in 100 μL PBS. 20 μL of the PBS solution subjected to assessment of exosome morphology and distribution by HT-7700 transmission electron microscopy (Hitachi, Japan). In addition, 10 μL of the PBS solution was used for particle size analysis of exocrine in N30Enanoparticle analyzer (NanoFCM, UK).
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