At 42 days of age, systolic blood pressure was determined in trained, conscious mice from both the vehicle and 1-2-NQ-exposed groups using the indirect tail-cuff method (Power Lab 4/S, ADInstruments, Dunedin, New Zealand). The mice were warmed at 40 °C for 5 min, and three stable consecutive blood pressure measurements were taken and averaged [55 (link)].
Power lab 4 s
Manufactured by ADInstruments
Sourced in Australia
The PowerLab 4/S is a versatile data acquisition and analysis system designed for a wide range of applications in teaching and research laboratories. It features multiple channels for recording and analyzing physiological signals, with high-quality amplifiers and filters to ensure accurate data capture. The PowerLab 4/S supports a variety of sensor inputs and can be integrated with a range of specialized software packages for data processing and visualization.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using power lab 4 s
1
Measuring Systolic Blood Pressure in Mice
2
Flexor reflex EMG recordings after incision
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flexor reflex EMG recordings were performed 24 h after adult incision.9 (link), 12 (link) The animals were anaesthetised with isoflurane, ventilated via a tracheal tube, and supported in a spinal frame. Isoflurane was maintained at 1.2 vol% for 20 min before and during recordings to allow mechanical ventilation without excessive reflex suppression. Data were included from 82 of 88 animals that had stable body temperature, heart rate, and oxygen saturation, whilst six were excluded because of unstable physiology and poor recording conditions. A bipolar EMG electrode in the biceps femoris recorded activity for 12 s after plantar hind-paw mechanical stimuli (vFh number 14–20, 13–120 g) (Neurolog; Digitimer, Welwyn Garden City, UK; PowerLab 4S; ADInstruments, Bella Vista, Australia). The integral of the EMG response was plotted against vFh number, and the area under the stimulus–response curve (AUC) quantified the overall ‘reflex response’.9 (link), 12 (link)
3
Gastric Motility Recording in Anesthetized Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The animals were fasted overnight with free access to water. For anesthesia, 10% urethane (1.0–1.2 g/kg, via intraperitoneal route) was administered. Gastric motility was recorded by inserting a small balloon via an incision of duodenum into the pyloric area as described previously [10 ]. In order to detect the gastric contraction, 0.2-0.3 mL warm water was injected into the balloon to keep the basal pressure at about 100 mm H2O.
Changes in pressure of the balloon were measured continuously by a transducer and then input into a polygraph amplifier (NeuroLog, NL900D). The signal was captured online and analyzed offline using a data acquisition system (Power-Lab/4s, AD Instruments) and Chart 5.2 software. Gastric contraction was recorded as a control for at least 30 min before any stimulation. Responses induced by MAS or LMS were compared with the background activity in terms of average amplitude (the average difference between the cyclic maxima and minima in the selected cycles), integral (calculated as the sum of the data points multiplied by the sample interval), and frequency (per minute) of gastric contraction waves. Systemic blood pressure and heart rate were monitored by using Biopac data acquisition system (MP150, USA), and rectal temperature was kept constantly around 37°C by a feedback-controlled heating blanket (DC, USA).
Changes in pressure of the balloon were measured continuously by a transducer and then input into a polygraph amplifier (NeuroLog, NL900D). The signal was captured online and analyzed offline using a data acquisition system (Power-Lab/4s, AD Instruments) and Chart 5.2 software. Gastric contraction was recorded as a control for at least 30 min before any stimulation. Responses induced by MAS or LMS were compared with the background activity in terms of average amplitude (the average difference between the cyclic maxima and minima in the selected cycles), integral (calculated as the sum of the data points multiplied by the sample interval), and frequency (per minute) of gastric contraction waves. Systemic blood pressure and heart rate were monitored by using Biopac data acquisition system (MP150, USA), and rectal temperature was kept constantly around 37°C by a feedback-controlled heating blanket (DC, USA).
4
Hypertension Induction with Angiotensin II
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The induction of hypertension with ANG II was performed as previously described [12] (link). Briefly, rats were anesthetized with xylazine (7.4 mg/Kg, ip) and ketamine (113 mg/Kg, ip) and osmotic minipumps (Alzet 2002) containing either ANG II 400 ng/Kg/min (ANG II group, n = 11) or saline 0.9% (control group, n = 11) were subcutaneously implanted in the animals. In another group, the des-Arg9-Leu8-bradykinin, a selective B1R antagonist [13] (link), [14] (link), was simultaneously infused with ANG II (ANG II + DAL group, n = 10). The B1R antagonist was infused subcutaneously by osmotic minipumps at a rate of 350 ng/Kg/min. All infusions were performed for 14 days [15] (link). In another series of experiments, ANG II infused-rats, were simultaneously treated with losartan (10 mg/kg by gavage for 14 days; ANG II+LOS; n = 5). Systolic arterial pressure was measured in conscious rats by tail-cuff plethysmography (PowerLab 4/S, AD Instruments Pty Ltd) at zero, 7, and 14 days after the minipump implantation, as previously described [12] (link) and calculated as the average of three consecutive measurements.
5
Measuring Systolic Blood Pressure in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baseline systolic blood pressure was determined before the experiments in conscious male twelve-week-old SHR and Wistar rats by an indirect tail-cuff plethysmography (pneumatic transducer, Power Lab 4/S, AD Instruments Pty Ltd) as previously described18 (link). Briefly, rats were preheated at 40 °C for 5 min and systolic blood pressure was defined as the moment that a definitive pulse could be detected. The experiment was conducted in an isolated and quiet room. Results were calculated as an average of three consecutives measurements and expressed as millimeters of mercury (mmHg). Care was taken in selecting an appropriate cuff size for each animal. A systolic blood pressure ≥140 mmHg was considered hypertensive, and ≤125 mmhg normotensive. For the other experiments presented in this work, 24–26-week-old male SHR and Wistar rats were used.
6
Indirect Tail-Cuff Blood Pressure Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Systolic blood pressure was determined in conscious rats using an indirect tail-cuff method (pneumatic transducer, PowerLab 4/S, AD Instruments Pty Ltd), a few days before experimentation. Rats were preheated at 40°C for 5 min, and three stable consecutive measurements of blood pressure were averaged.
7
Tail Plethysmography for Hypertension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The systolic blood pressure was measured by the noninvasive method of tail plethysmography. Cuffs coupled to pressure transducers were placed around the tails of the awake animals, which had been previously warmed in a cabinet at 37°C. The pressure change data were acquired using a Power Lab 4/S analog-to-digital converter (ADInstruments Pty Ltd.) and the results were presented as the average of three consecutive measurements for each animal. Prior to the first arterial pressure measurement, the animals were adapted to the procedure by placing them in the acrylic container on 5 consecutive days. The arterial pressure and body weight were determined on a weekly basis during the 4 weeks of the study. The model was deemed to have been successfully established if the systolic blood pressure was higher than 160 mmHg after 4 weeks of operation.
8
Noninvasive Blood Pressure Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Resting BP and HR were noninvasively determined using a computerized tail-cuff system (PowerLab 4/S ADInstruments Pty Ltd, Castle Hill, Australia) prior to and following the aerobic training protocol. Rats were acclimatized to the apparatus during daily sessions over 5 days, 1 week prior to the commencement of the experimental period.
9
Goldblatt's 2-Kidney, 1-Clip Hypertension Model in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Renovascular hypertension 2-kidney, 1-clip (model, 2K1C) was induced as previously described by Goldblatt et al. [17 (link)]. Male Wistar rats weighing 180–200 g (n = 17) were anesthetized intraperitoneally with ketamine (100 mg/kg) and xylazine (10 mg/kg) and a U-shaped silver clip with an internal diameter of 0.2 mm was placed around the left renal artery (2K1C/hypertensive group, n = 10). The Sham group (normotensive, n = 7) performed the same surgical procedure, but except the insertion of the clip into the renal artery.
Systolic blood pressure (SBP) was evaluated using tail plethysmography, in the awake animals after they were heated in a cabinet at 37 °C for 15 min. The pressure change data were captured by a Power Lab 4/S analog-to-digital converter (AD Instruments Ltd., Castle Hill, Australia) and the results were represented as the average of three consecutive measurements for each animal. Before the first measurement, for 5 days, the animals were adapted to the procedure to minimize stress-induced SBP fluctuations. The animals were considered hypertensive when systolic blood pressure was above 160 mmHg [18 (link)]. In this study, 17% of animals were not considered hypertensive. Systolic blood pressure and body mass were evaluated weekly for 4 weeks, and then the animals were euthanized, and the organs removed.
Systolic blood pressure (SBP) was evaluated using tail plethysmography, in the awake animals after they were heated in a cabinet at 37 °C for 15 min. The pressure change data were captured by a Power Lab 4/S analog-to-digital converter (AD Instruments Ltd., Castle Hill, Australia) and the results were represented as the average of three consecutive measurements for each animal. Before the first measurement, for 5 days, the animals were adapted to the procedure to minimize stress-induced SBP fluctuations. The animals were considered hypertensive when systolic blood pressure was above 160 mmHg [18 (link)]. In this study, 17% of animals were not considered hypertensive. Systolic blood pressure and body mass were evaluated weekly for 4 weeks, and then the animals were euthanized, and the organs removed.
10
Cardiac Function Monitoring During Ischemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following functional parameters were continuously monitored with a computer-based data acquisition system (PowerLab/4S with Chart 5 software, AD Instruments, Gladstone, Australia): left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP, LVDP = LVSP-LVEDP), maximum rise/down velocity of left intraventricular pressure (dp/dtmax and dp/dtmin), coronary flow (CF) and heart rate (HR). The heart effluents were collected at 1 min intervals to determine the coronary flow (CF). The recovery of LVDP, dp/dtmax, dp/dtmin, CF and HR were expressed as the percent of 1 minute before ischemia.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!