Antemortem blood samples were collected from pigs for serum preparation. Each pig was properly restrained as described in the ILRI Standard Operating Procedures (SOPs) manual, Sect. 2 , part (c) & (d) [20 ]. Blood was collected from the jugular vein using a 21G, 1.5" needle into plain 5 mL BD® vacutainer tubes. The tubes were labeled and then placed in an ice box containing ice packs at 4 °C. After collection, the samples were delivered to the district veterinary laboratory, where they were left to stand at room temperature (20 °C) overnight. After 24 h, sera were harvested into 2 mL cryotubes (Sarstedt®, Germany), labeled and stored in a freezer at − 20 °C until use.
2 ml cryo tubes
Manufactured by Sarstedt
Sourced in Germany
The 2-ml cryo-tubes from Sarstedt are made of durable polypropylene material and designed for cryogenic storage applications. They have a screw cap closure and are suitable for freezing and storing samples at low temperatures.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using 2 ml cryo tubes
1
Pig Blood Sampling for Serum Preparation
2
Porcine Euthanasia and Fecal Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On days 18 and 19, pigs were euthanized 2 h after their last feeding via intracardiac injection of T61 (10 ml/kg Embutramide, MSD animal Health, Vienna, Austria) after general anesthesia was induced (Narketan 100 mg/ml, 1 ml/10 kg body weight, Ketaminhydrochlorid, Vétoquinol GmbH, Germany; Stresnil 40 mg/ml; 0.5 ml/10 kg body weight, Azaperon, Elanco Deutschland GmbH, Germany). For the analysis of metabolic active bacteria, fresh feces from the rectum were aseptically collected in 2-ml cryo-tubes (Sarstedt AG & Co., Nümbrecht, Germany), immediately snap-frozen in liquid nitrogen and stored at −80°C until analysis. In one pig, the rectum was empty and digesta could not be collected for microbial analysis.
3
Monitoring Respiratory Pathogens in Pigs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pigs were monitored for exposure to 4 respiratory pathogens by blood sampling at each visit. Larger pigs were restrained with a metallic pig catcher (Model BZ002, MG® Livestock, Shandong, China), while smaller pigs were restrained by hand. With a pig properly restrained, blood was collected from the cranial vena cava or jugular vein, using a 21G, 1.5″ needle into 5 mL plain BD® vacutainer tubes. The tubes were labeled with pig details and placed in icebox at 4–6°C. Shortly after collection, samples were delivered to Lira district veterinary laboratory for temporary storage. Blood samples were left to stand at room temperature (20°C) overnight and serum harvested the next day into 2 mL cryotubes (Sarstedt®, Germany), labelled and stored in a fridge at −20°C until testing.
4
Cecal Microbiome DNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To obtain samples for microbial genes analysis, fresh samples from the cecum (n = 11/ group) were aseptically collected 5–10 min after slaughter under anaerobic conditions and placed into sterile 2-ml cryo-tubes (Sarstedt, Nümbrecht, Germany), then, samples were immediately stored at −80°C until total microbial DNA extraction. Then, all the sample tubes were instantaneously snap-frozen in liquid nitrogen, and subsequently stored at −80°C for extraction of total microbial DNA later.
The microbial DNA was extracted from 300-μl cecal samples using a DNA stool mini kit (QIAmp DNA Stool mini Kit; QIAGEN, Hilden, Germany) following the manufacturer’s instructions. A Qubit 2.0 Fluorometer (Thermo Fisher Scientific Co., Ltd., Shanghai, China) with a Qubit dsDNA HS assay kit (Life Technologies) was used to quantify DNA concentration.
The microbial DNA was extracted from 300-μl cecal samples using a DNA stool mini kit (QIAmp DNA Stool mini Kit; QIAGEN, Hilden, Germany) following the manufacturer’s instructions. A Qubit 2.0 Fluorometer (Thermo Fisher Scientific Co., Ltd., Shanghai, China) with a Qubit dsDNA HS assay kit (Life Technologies) was used to quantify DNA concentration.
5
Porcine Jugular Vein Blood Collection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At ante-mortem, blood samples were collected from pigs for sera preparation. Each pig was properly restrained as described in the ILRI Standard Operating Procedures (SOPs) manual, Sect. 2, part (c) & (d) [19] . Blood was collected from the jugular vein, using a 21G, 1.5" needle into plain 5 mL BD® vacutainer tubes. The tubes were labeled and then placed in an ice box containing ice packs at 4°C. After collection, samples were delivered to the district veterinary laboratory, where they were left to stand at room temperature (20°C) overnight. After 24 hours, sera were harvested into 2 mL cryotubes (Sarstedt®, Germany), labelled and stored in a fridge at -20°C until use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!