Periscan pim3 system

Cerebral blood flow (CBF) (n = 8) was measured using laser speckle perfusion image system (PeriScan PIM3 System; PERIMED, Stockholm, Sweden). In short, rats were anesthetized with pentobarbital sodium (0.1 g/kg body weight, i.p.), with an incision made through the scalp, and the skin was retracted to expose the skull. The periosteal connective tissue adherent to the skull was removed with a sterile cotton swab. A parietal bone window of 3 × 5 mm² was opened with a hand-held drill on the right side 1 mm behind the coronal suture, and 1 mm lateral to sagittal suture as per described protocol (Xu et al., 2009 (link)). A low-powered He/Ne laser beam over the exposed parietal bone was directed by a computer-controlled optical scanner. The distance between the scanner head and cerebral cortex was 18.5 cm, with the scanner head parallel to the cerebral cortex surface. At each measuring site, the beam illuminated the tissue to a depth of 0.5 mm, as set in the instrument (Gu et al., 2018 (link)), and images were acquired after 10 min of basic observation. A color-coded image to denote specific relative perfusion level was displayed on a video monitor, and all images were evaluated with the software LDPI win 3.1 (PeriScan PIM3 System; PERIMED, Stockholm, Sweden), by which the number of perfusion unit for each image was calculated automatically.

Cerebral blood flow (CBF) was measured using Laser Doppler perfusion image system (PeriScan PIM3 System; PERIMED, Stockholm, Sweden) as previously described29 (link) with some modifications. Briefly, after retracing the scalp, a low-powered He-Ne laser beam was directed by computer-controlled optical scanner over the exposed cortex with the scanner head positioned in parallel to the cerebral cortex at a distance of 18.5 cm. The CBF was measured before the cerebral ischemia (baseline), 10 min and 80 min after ischemia, 10 min and 24 h after reperfusion, respectively. A color-coded image denoting specific relative perfusion levels was displayed on a video monitor and the ischemia core area in all images was evaluated with the software LDPIwin 3.1 (PeriScan PIM3 System; PERIMED, Stockholm, Sweden). Only those mice that had a CBF lower than 20% of baseline at 10 min and 80 min after ischemia and higer than 70% of baseline at 10 min after reperfusion were considered as successful induction of focal cerebral I/R.