Blood from 3 healthy donors was used to separate peripheral blood mononuclear cells, using Ficoll-Paque separation media (GE Healthcare). MLR was essentially performed as previously described.38 (link) Further details are in the supplemental Material and methods. After 48 hours, all samples were treated for 5 hours with Golgi plug protein transport inhibitor, brefeldin (BD Biosciences) to inhibit cytokine accumulation in the Golgi complex and processed to measure interferon-γ (IFN-γ) production by CD4+ and CD8+ T cells by flow cytometry.
Ficoll paque separation media
Manufactured by GE Healthcare
Ficoll-Paque separation media is a density gradient medium used for the separation and isolation of different cell types, such as mononuclear cells, from whole blood or other biological samples. It functions by allowing the various components of the sample to separate based on their unique densities when centrifuged.
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Lab products found in correlation
2 protocols using ficoll paque separation media
1
Measuring IFN-γ production in T cells
2
Isolation of Leukemia and Normal Cells
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Primary leukemia specimens (n = 3) were obtained from ALL patients upon ethical approval from Weill Cornell Medicine Institutional Review Board (IRB; # 0909010629, #0910010677). De-identified normal peripheral blood (NPB) samples (n = 3) were purchased from the New York Blood Center (New York, NY). Mononuclear cells (MNCs) were isolated by density separation using the Ficoll-Paque separation media (GE Healthcare, #17-1440-03), as manufacturer instructions, and cryo-stored in liquid nitrogen until use.
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