For immunophenotype characterization, PDLSCs were incubated with antihuman stem cell surface-labeled antibodies including CD105-phycoerythrin, CD45-phycoerythrin, CD146-phycoerythrin, CD14-phycoerythrin, CD29-phycoerythrin, and stro1-phycoerythrin (BD Biosciences, USA). All flow cytometry tests were performed on a FACSAria (BD Bioscience).
Cd146 phycoerythrin
Manufactured by BD
Sourced in United States
CD146-phycoerythrin is a fluorescent-labeled antibody used in flow cytometry applications. It binds to the CD146 cell surface antigen, which is expressed on various cell types. This product can be used to identify and quantify CD146-positive cells in biological samples.
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Lab products found in correlation
Cd45 phycoerythrin, by BD (1 mentions)
Cd105 phycoerythrin, by BD (1 mentions)
Cd14 phycoerythrin, by BD (1 mentions)
Facsaria, by BD (1 mentions)
Cd24 fluorescein isothiocyanate fitc, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Cd45 fitc, by BD (1 mentions)
Alizarin red staining, by Cyagen (1 mentions)
Oil red o, by Cyagen (1 mentions)
2 protocols using cd146 phycoerythrin
1
Flow Cytometric Phenotyping of PDLSCs
2
Isolation and Characterization of SCAP
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Human SCAP were isolated from an extracted immature mandibular third molar of a male patient aged 18 years old by a method used in a previous study (11 (link)). All protocols were reviewed and approved by the Ethics Committee of Guanghua School and Hospital of Stomatology, Sun Yat-sen University (Guangzhou, China). The osteogenic and adipogenic differentiation capacities of SCAP were identified by Alizarin Red staining and Oil Red O staining (Cyagen Biosciences, Inc., Guangzhou, China), respectively (11 (link)). The typical phenotypes, including STRO-1/Alexa Fluor 647-Allophycocyanin (BioLegend, Inc., San Diego, CA, USA), CD146/Phycoerythrin (BD Pharmingen, San Diego, CA, USA), CD24/Fluorescein Isothiocyanate (FITC) (BD Pharmingen) and CD45/FITC (BD Pharmingen), using the 2nd passage of SCAP were assessed using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
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