Trehalose dihydrate (#T0167), vinyl acrylate (#771422), Lipase B acrylic resin from Candida antarctica (CALB) (#L4777), trimethylopropane ethoxylate (#416177), ethyl thiolactate (#W327905), ethyl thioglycolate (#E34307), polyethylene diacrylate (PEGDA) (Mn = 575 g mol−1) (#437441), activated alumina Brockman I (basic and neutral), Filter agent Celite 545, solvents (dichloromethane, methanol, ethyl acetate, acetone, dimethyl sulfoxide), 3A and 4A molecular sieves, horseradish peroxidase (#77332, 156 U mg−1), glucose oxidase from Aspergillus Niger (#49180, 192 U mg−1), α-Chymotrypsin from bovine pancreas (#C3142), 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (#T0440), d -(+)-glucose anhydrous, N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (#S7388, chymotrypsin substrate), 2 n HCl solution, Tris hydrochloride (Tris-HCl), calcium chloride (CaCl2) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline pH = 7.4, FITC labeled ovalbumin, IgG-Alex Fluor 647 and Amplex UltraRed reagent were purchased from Life Technologies (Grand Island, NY, USA). Disposable 40 and 80 g HP Silica Gold Cartridges were purchased from Teledyne Isco (Lincoln, NE, USA).
3 3 5 5 tetramethylbenzidine tmb liquid substrate system for elisa
Manufactured by Merck Group
Sourced in United States
3,3',5,5'-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA is a colorimetric reagent used in enzyme-linked immunosorbent assay (ELISA) applications. It serves as a substrate for the detection of enzyme activity, producing a colored reaction product that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Trehalose dihydrate, by Merck Group (1 mentions)
Igg alex fluor 647, by Thermo Fisher Scientific (1 mentions)
Amplex ultrared reagent, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline ph 7.4, by Thermo Fisher Scientific (1 mentions)
Lipase b acrylic resin, by Merck Group (1 mentions)
α chymotrypsin from bovine pancreas, by Merck Group (1 mentions)
D glucose anhydrous, by Merck Group (1 mentions)
Horseradish peroxidase, by Merck Group (1 mentions)
N succinyl ala ala pro phe p nitroanilide, by Merck Group (1 mentions)
Cellulose dialysis tubing, by Thermo Fisher Scientific (1 mentions)
Nanodrop onec spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline ph 7, by Thermo Fisher Scientific (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Lb broth, by Merck Group (1 mentions)
Nunc maxisorp plate, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Carl Roth (1 mentions)
Stop reagent for tmb substrate, by Merck Group (1 mentions)
3 protocols using 3 3 5 5 tetramethylbenzidine tmb liquid substrate system for elisa
1
Enzymatic Activity Assays Development
2
Enzyme Immobilization for Bioassays
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Collagenase from Clostridium
histolyticum (Type I, ≥125 CDU
mg–1 solid), alginate lyase (≥10,000 units/g
solid), peroxidase from horseradish (HRP, Type VI, ≥250 units/mg
solid), trypsin (≥10,000 BAEE units/mg protein), thrombin (lyophilized
powder, 40–300 NIH units/mg protein), and chymotrypsin (Type
II, lyophilized powder, ≥40 units/mg protein). 3,3′,5,5′-Tetramethylbenzidine
(TMB) liquid substrate system for ELISA, sodium alginate, LB broth,
bovine serum albumin (BSA), and gelatin for electrophoresis were from
Sigma-Aldrich, USA. Peptide sequence (H2N-GGGLGPAGGK-OH)
was synthesized and purified by Synpeptide Co. Ltd., China. Phosphate-buffered
saline (PBS, pH 7.4) and cellulose dialysis tubing were from Gibco,
Thermo Fisher Scientific, USA. Sodium periodate (NaIO4),
sodium cyanoborohydride (NaBH3CN), dimethyl sulfoxide (DMSO),
and γ-amino-propyl-trimethoxy silane (APTES) were from J&K
Scientific Ltd., China. Tris(hydroxymethyl)aminomethane (Tris), polyethylene
glycol-400 (PEG-400), acetic anhydride, and other general chemicals
were from Dieckmann Co. Ltd., China. Solvents for synthesis were of
analytical grade unless specified otherwise. Glass substrates for
enzyme immobilization were from Sail Brand, China. UV–vis spectrophotometry
was conducted on a NanoDrop One C spectrophotometer, Thermo Fisher
Scientific, USA.
histolyticum (Type I, ≥125 CDU
mg–1 solid), alginate lyase (≥10,000 units/g
solid), peroxidase from horseradish (HRP, Type VI, ≥250 units/mg
solid), trypsin (≥10,000 BAEE units/mg protein), thrombin (lyophilized
powder, 40–300 NIH units/mg protein), and chymotrypsin (Type
II, lyophilized powder, ≥40 units/mg protein). 3,3′,5,5′-Tetramethylbenzidine
(TMB) liquid substrate system for ELISA, sodium alginate, LB broth,
bovine serum albumin (BSA), and gelatin for electrophoresis were from
Sigma-Aldrich, USA. Peptide sequence (H2N-GGGLGPAGGK-OH)
was synthesized and purified by Synpeptide Co. Ltd., China. Phosphate-buffered
saline (PBS, pH 7.4) and cellulose dialysis tubing were from Gibco,
Thermo Fisher Scientific, USA. Sodium periodate (NaIO4),
sodium cyanoborohydride (NaBH3CN), dimethyl sulfoxide (DMSO),
and γ-amino-propyl-trimethoxy silane (APTES) were from J&K
Scientific Ltd., China. Tris(hydroxymethyl)aminomethane (Tris), polyethylene
glycol-400 (PEG-400), acetic anhydride, and other general chemicals
were from Dieckmann Co. Ltd., China. Solvents for synthesis were of
analytical grade unless specified otherwise. Glass substrates for
enzyme immobilization were from Sail Brand, China. UV–vis spectrophotometry
was conducted on a NanoDrop One C spectrophotometer, Thermo Fisher
Scientific, USA.
3
SARS-CoV-2 Spike Antibody ELISA Assay
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+ Expand
SARS-CoV-2 spike-specific antibodies were measured by ELISA as described previously [19 (link),20 (link),24 (link)]. Briefly, flat bottom 96-well ELISA plates (Nunc MaxiSorp Plates, Thermo Fisher Scientific, Karlsruhe Germany) were coated with 50 ng/well of one of the SARS-CoV-2 spike antigens listed in Supplementary Table S1 (from ACROBiosystems, Newark DE, USA and The Native Antigen Company, Kidlington, UK) overnight at 4 °C. Mouse sera were three-fold serially diluted in PBS containing 1% BSA (Carl Roth GmbH, Karlsruhe, Germany) (PBS/BSA), transferred to the coated ELISA plates and incubated for 1 h at 37 °C. Plates were then probed with goat anti-mouse IgG HRP diluted in PBS/BSA. SARS-CoV-2 spike-specific antibodies were detected using 3′3′,5′5′- Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, Taufkirchen, Germany), and the reaction was stopped using Stop Reagent for TMB Substrate (Sigma-Aldrich). The absorbance was measured at 450 nm with a 620 nm reference wavelength using the Spark® multimodal microplate reader (Tecan, Männedorf, Switzerland). Data were normalized using a positive control sample. The cut-off value for positive mouse serum samples was determined by calculating mean OD 450 nm values of the PBS control group sera plus six standard deviations (mean + 6 SD).
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