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47 protocols using kaleidagraph version 4

1

Quantitative Data Analysis Protocol

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Quantitative data are presented as the mean ± standard deviation (SD). Student’s t-tests (unpaired and two-tailed) were used for two-group comparisons (KaleidaGraph Version 4.0, Synergy Software, Reading, PA, USA). p < 0.05 was considered significant.
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2

Clinical Trial Statistical Analysis

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Statistical analyses were performed using SAS version 9.2 (SAS Institute Inc., Cary, NC, USA). The target sample size of 57 (52 eligible patients and 10 % ineligible patients) was based on expected and threshold response rates of 62 and 40 %, respectively, with α = 0.05 and β = 0.1. P < 0.05 was considered significant. Graphs were created using KaleidaGraph version 4.0 (Synergy Software, Reading, PA, USA), Photoshop CS2 (Adobe Systems, San Jose, CA, USA) and IBM SPSS Statistics 19 (IBM, Armonk, NY, USA).
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3

Protein Folding Kinetics Analysis

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Folding and unfolding kinetics were followed by recording intrinsic fluorescence changes upon transition at 298 K in a Bio-Logic SFM-3 stopped-flow instrument (Bio-Logic Science Instruments), employing an excitation wavelength of 280 nm and a 320 nm emission cut-off filter. The protein concentration was 20 μM in 50 mM sodium acetate at pH 5.7 with or without urea. All fluorescence traces fitted well to a single exponential function, and kinetic parameters were derived to a two-state folding model, using the Kaleidagraph version 4.0 (Synergy Software), as previously described [18] (link).
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4

Statistical Analysis of Experimental Data

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Results are presented as means ± standard error mean. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, PA, USA) as described previously10 (link). Significant differences were accepted at p < 0.05.
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5

Statistical Analysis of Experimental Data

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Results are presented as means ± S.E.M. Differences between groups were analyzed using one-way analysis of variance, and corrections for multiple comparison were made using Tukey’s multiple comparison test. Comparisons between two groups were made using Student’s t-test. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Significant differences were assumed at p < 0.05.
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6

Comparative Statistical Analysis of Experimental Data

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The data are presented as means ± standard error of the mean. Differences between groups were analyzed using one-way analysis of variance, and corrections for multiple comparison were made using Tukey’s multiple comparison test. Comparisons between two groups were made using Student’s t-test. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Differences were assumed as significant at p < 0.05.
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7

Statistical Analysis of Experimental Data

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Results are presented as means ± S.E.M. Differences between groups were analyzed by one-way analysis of variance, and corrections for multiple comparisons were made using Tukey’s or Dunnett’s test for multiple comparison. Comparisons between two groups were made using Student’s t test. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Significant differences were assumed at p < 0.05.
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8

Statistical Analysis of Experimental Data

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Results are presented as means ± S.E.M. Differences between groups were analyzed by one-way analysis of variance, and corrections for multiple comparison were made using Tukey’s multiple comparison test. Comparisons between two groups were made using a Student’s t-test. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Significant differences were assumed at p < 0.05.
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9

Statistical Analysis of Experimental Data

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Results are presented as means ± S.E.M. Each study was repeated at least three times. Differences between groups were analyzed by one-way analysis of variance, and corrections for multiple comparisons were made using Tukey’s multiple comparison test. Comparisons between two groups were made using Student’s t-test. Statistical analyses were performed using KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Significant differences were assumed at p < 0.05.
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10

Pharmacological Effects of Novel Compound

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Results are presented as mean ± standard error. Differences between groups were analyzed using one-way analysis of variance, and corrections for multiple comparisons were made using Dunnett’s test or Tukey’s multiple comparison test. Comparisons between the two groups were made using Student’s t-test. Statistical analyses were performed using the KaleidaGraph version 4.5.1 software (Synergy Software, Reading, PA, USA). Differences were considered to be significant at p < 0.05.
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