4500 q trap

Manufactured by AB Sciex

Sourced in United States, Japan

The 4500 Q TRAP is a hybrid triple quadrupole linear ion trap mass spectrometer designed for a wide range of analytical applications. It features enhanced sensitivity, resolution, and scanning speed to support high-throughput analysis. The 4500 Q TRAP provides accurate and reproducible quantitative data for small molecules and peptides.

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Lab products found in correlation

Exion lc, by AB Sciex (2 mentions) Exigent autosampler, by AB Sciex (2 mentions) Turbo 5, by AB Sciex (2 mentions) Alpha rvc, by Martin Christ (1 mentions) Oasis mcx, by Waters Corporation (1 mentions) Drx 500 spectrometer, by Bruker (1 mentions) Hypersil gold, by Thermo Fisher Scientific (1 mentions) Cms l compact mass spectrometer, by Advion (1 mentions) Sb c18 column, by Agilent Technologies (1 mentions) Shim pack uflc cbm30a, by Shimadzu (1 mentions) Acquity uplc hss t3 c18 column, by Waters Corporation (1 mentions) Analyst 1, by AB Sciex (1 mentions) Cbm30a system, by Thermo Fisher Scientific (1 mentions) Prominence uflc, by Shimadzu (1 mentions) Beh c18, by Waters Corporation (1 mentions) Shim pack uflc cbm30a system, by Shimadzu (1 mentions) Ascentis express hilic column, by Merck Group (1 mentions) Atlantis c18 column, by Waters Corporation (1 mentions)

Close spelling variants of this product

API 4500 Q TRAP UPLC/MS/MS System (10 citations)

11 protocols using 4500 q trap

Cytokinin Extraction and Analysis

Cited 1 time

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Plant materials were homogenized in liquid nitrogen and placed in an extraction mixture consisting of methanol/water/formic acid. The supernatants were evaporated in a vacuum concentrator (Alpha RVC, Christ, Osterode, Germany), and were then applied to a mixed mode reversed phase-cation exchange SPE column (Oasis-MCX, Waters, Milford, MA, USA), as previously described [52 (link)]. The cytokinin fraction was sequentially eluted, evaporated, and finally dissolved in 5% MeOH. An ultra-performance liquid chromatography (1290, Agilent, Pal Alto, CA, USA) coupled to a hybrid triple quadrupole/linear ion trapmass spectrometer (4500 Q TRAP, AB SCIEX, Waltham, MA, USA) was used to analyze each aliquot.

Feng Y., Sun Q., Zhang G., Wu T., Zhang X., Xu X., Han Z, & Wang Y. (2019). Genome-Wide Identification and Characterization of ABC Transporters in Nine Rosaceae Species Identifying MdABCG28 as a Possible Cytokinin Transporter linked to Dwarfing. International Journal of Molecular Sciences, 20(22), 5783.

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HPLC-MS/MS Analysis of Dissolved Samples

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HPLC–MS/MS analyses were performed by ExionLC (AB Sciex) liquid chromatograph equipped with an Exigent autosampler (AB Sciex) coupled with tandem mass spectrometer (4500 QTRAP, AB Sciex) with electrospray ion source (Turbo V, AB Sciex). An amount of 2 µL of the dissolved sample was injected for analysis into ACE Excel C18 column with dimensions of 2.1 mm ×50 mm × 1.7 µm. Flow ratio of the column was 0.4 mL/min with a temperature of 40 °C. H₂O with 0.5mM HFBA and MeOH in proportion with (1:1) ACN constituted, respectively, eluents A and B. The time and used a gradient of the eluents are following: O min. eluent A: 80%, eluent B: 20%; time 7 min. eluent A: 50%, eluent B: 50%; time 7.1–9 min. eluent A: 5%, eluent B: 95%; 9.1–11 min. eluent A: 80%, eluent B: 20%.

Burzynska-Pedziwiatr I., Jankowski A., Kowalski K., Sendys P., Zieleniak A., Cypryk K., Zurawska-Klis M., Wozniak L.A, & Bukowiecka-Matusiak M. (2020). Associations of Arginine with Gestational Diabetes Mellitus in a Follow-Up Study. International Journal of Molecular Sciences, 21(21), 7811.

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Synthesis and Characterization of Organic Compounds

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The purity of all final compounds was >95 % purity as assessed by HPLC. Final compounds were analyzed on an Agilent 1200 series chromatograph. The chromatographic method utilized as ThermoScientific Hypersil GOLD C-18 4.6 × 250 mm, 3 μm column. UV detection wavelength = 220 nm; flow-rate = 1.0 mL/min; gradient = 5 - 95% acetonitrile over 12 min and 3 min hold time at 95% acetonitrile. Both organic and aqueous mobile phases contain 0.1% v/v formic acid. The mass spectrometer used is an AB Sciex 4500 QTrap triple-quadrupole mass spectrometer with an ESI source or an Advion CMS-L Compact Mass Spectrometer with an ESI or an APCI source. Samples are submitted for analysis solubilized in 1:1 acetonitrile:water solution or using the atmospheric solids analysis probe (ASAP). ¹H and ¹³C NMR spectra were recorded on either Bruker DRX500 spectrometer (operating at 500 and 125 MHz, respectively) or Bruker AVIII (operating at 800 and 200 MHz, respectively) in DMSO-d₆ or CDCl₃ with or without the internal standard of TMS at 0.05% v/v. The chemical shifts (δ) reported as parts per million (ppm) and the coupling constants are reported as s = singlet, bs = broad singlet, d = doublet, t = triplet, q = quartet, dd = doublet of doublet, m = multiplet. Compounds were generally prepared according to scheme 1 and protocols are detailed below. Intermediate 4C was commercially available.

Kaur J., Soto-Velasquez M., Ding Z., Ghanbarpour A., Lill M.A., van Rijn R.M., Watts V.J, & Flaherty D.P. (2018). Optimization of a 1,3,4-oxadiazole series for inhibition of Ca2+/calmodulin-stimulated activity of adenylyl cyclases 1 and 8 for the treatment of chronic pain. European journal of medicinal chemistry, 162, 568-585.

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HPLC-MS/MS Analysis of Compounds

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HPLC-MS/MS analyses were performed by ExionLC (AB Sciex) liquid chromatograph equipped with an Exigent autosampler (AB Sciex) coupled with tandem mass spectrometer (4500 QTRAP, AB Sciex) with electrospray ion source (Turbo V, AB Sciex). 2 µL of the dissolved sample was injected for analysis into ACE Excel C18 column with dimensions of 2.1 mm × 50 mm × 1.7 µm. Flow ratio of the column was 0.4 mL/min with the temperature 40 °C. H₂O with 0.5 mM HFBA and MeOH in proportion with (1:1) ACN constituted respectively, eluents A and B. The time and used gradient of the eluents are shown in Table 1.

Bobeff E.J., Bukowiecka-Matusiak M., Stawiski K., Wiśniewski K., Burzynska-Pedziwiatr I., Kordzińska M., Kowalski K., Sendys P., Piotrowski M., Szczesna D., Stefańczyk L., Wozniak L.A, & Jaskólski D.J. (2022). Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage. Journal of Clinical Medicine, 11(2), 380.

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UPLC-ESI-MS/MS Analysis of Prepared Samples

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The prepared sample extraction was analyzed employing a UPLC–ESI–MS/MS system, including a UPLC system (Shim-pack UFLC SHIMADZU CBM30A, Shimadzu, Kyoto, Japan) and an ESI–MS/MS system (Applied Biosystems 4500 Q TRAP, AB SCIEX, Foster City, CA, USA). A separation operation was implemented using a 1.8 µm Agilent SB-C18 column (100 mm × 2.1 mm) equilibrated with mobile phases A and B prepared by mixing ultrapure water with 0.1% formic acid and acetonitrile, respectively. Chromatographic separation was completed by way of a gradient elution program. First, mobile phase B rose linearly from 5% to 95% within 9 min. It was maintained at the level of 95% from 9 to 10 min, decreased to 5% from 10 to 11 min, and held steady until 14 min. The temperature of the column oven was 40 °C, the injection volume was 4 µL, and the flow rate was 0.35 mL/min [47 (link)]. The effluent was alternatively connected to an ESI–triple quadrupole-linear ion trap (QTRAP)-MS.

Lan Q., Liu C., Wu Z., Ni C., Li J., Huang C., Wang H, & Wei G. (2022). Does the Metabolome of Wild-like Dendrobium officinale of Different Origins Have Regional Differences?. Molecules, 27(20), 7024.

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Quantification of Gibberellin Hormones

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Plant material (~150 mg FW) was frozen in liquid nitrogen, ground into powder, and extracted with 1 ml of 80% methanol at 4 °C for 12 h. The extract was centrifuged at 12 000 g at 4 °C for 15 min. The supernatant was collected, evaporated to dryness under a nitrogen gas stream, and reconstituted in 100 ml of 95% acetonitrile. The solution was centrifuged again at 12 000 g at 4 °C for 15 min, and the supernatant was collected for LC-MS analysis. The sample extracts were analysed using an LC/MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 4500 Q TRAP) controlled by Analyst 1.6 software (AB Sciex). The HPLC was carried out on a Waters ACQUITY UPLC HSS T3 C18 column (1.8 µm I.D., 2.1 × 100 mm). Solvent A was water with 0.04% acetic acid and solvent B was acetonitrile with 0.04% acetic acid. The gradient program was 100% A at 0 min, ramped to 5% A by 11.0 min, back to 95% A by 12.1 min, and held until 15.0 min. The flow rate was 0.4 ml min^–1. The temperature was set at 40 °C. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS. Several GAs and GA intermediates were further analysed, namely GA₁, GA₄, GA₇, GA₉, GA₁₅, GA₁₉, GA₂₀, GA₂₄, and GA₅₃.

Yang T., Sun Y., Wang Y., Zhou L., Chen M., Bian Z., Lian Y., Xuan L., Yuan G., Wang X, & Wang C. (2020). AtHSPR is involved in GA- and light intensity-mediated control of flowering time and seed set in Arabidopsis. Journal of Experimental Botany, 71(12), 3543-3559.

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Quantification of Rice-Koji Compound

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Rice-koji (High dose: 0.5 g/mL) was diluted with 50% acetonitrile solution and filtered through a 0.2 μm membrane filter. The quantitative value of the sample was obtained by the internal standard method. EGT was quantified by liquid chromatography–tandem mass spectrometry (LC-MS/MS). Prominence UFLC (Shimadzu Corporation, Kyoto, Japan) was used as the liquid chromatography instrument, and a ZORBAX Rx-SIL (150 mm × 2.1 mm, 5.0 µm particle size) column was used at 40 °C. The injection volume was 5 µL. Mass spectrometry was performed using a 4500 Qtrap (AB SCIEX, Tokyo, Japan) with electrospray ionization.

Piriyaprasath K., Kakihara Y., Kurahashi A., Taiyoji M., Kodaira K., Aihara K., Hasegawa M., Yamamura K, & Okamoto K. (2023). Preventive Roles of Rice-koji Extracts and Ergothioneine on Anxiety- and Pain-like Responses under Psychophysical Stress Conditions in Male Mice. Nutrients, 15(18), 3989.

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Quantitative Lipid Analysis by UPLC-MS

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All vegetable oils (5 mg) were dissolved in solution (CHCl₃/methanol, 1/2, v/v) to 5 mg/mL, and then it was diluted (50 times) with methanol, which contained ammonium formate (10 mM), formic acid (0.1%) and internal standard (1,3(d5)-diheptadecanoyl-2-heptadecenoyl-glycerol (d5-(17:0/17:1/17:0) TAGs). For the setting condition of UPLC, mobile phase A was mixed with ACN and water (5:5, v/v), and mobile phase B was blended with IPA and ACN (9:1, v/v). They all contained ammonium formate (10 mM) and formic acid (0.1%). The chromatographic column was BEH C18 (1.7 μm, 2.1 mm ID × 100 mm, Waters Corporation, Milford, MA, USA), and the column temperature was 60 °C. The parameters of MS (4500QTrap, AB SCIEX, Framingham, MA, USA) are reported in detail in our previous works [3 (link)].

Zhu H., Zhao P., Wang X., Wang Y., Zhang S., Pang X, & Lv J. (2024). Fabrication of Human Milk Fat Substitute: Based on the Similarity Evaluation Model and Computer Software. Molecules, 29(9), 2096.

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UPLC-ESI-MS/MS Metabolite Profiling

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Sample extracts were analyzed using a UPLC-ESI-MS/MS system (UPLC: Shim-pack UFLC CBM30A system, Shimadzu, Kyoto, Japan; MS: Applied Biosystems 4500 QTRAP, AB Sciex, Framingham, MA, USA). The analytical conditions were as follows: Agilent SB-C18 UPLC column (1.8 µm, 2.1 mm × 100 mm; Agilent Technologies, Santa Clara, CA, USA) and a mobile phase comprising solvent A (pure water with 0.1% formic acid) and solvent B (acetonitrile). Sample measurements were performed using a gradient program that started with 95% A and 5% B. Within 9 min, a linear gradient with an endpoint of 5% A and 95% B was programmed, and the composition of 5% A and 95% B was maintained for 1 min. This was then reversed to 95% A and 5% B within 1.10 min, which was maintained for 2.9 min. The column oven temperature was set at 1–40 °C, and the injection volume was 4 μL. The effluent was connected to ESI-triple quadrupole linear ion trap (QTRAP)-MS.

, & Wang Y. (2021). A draft genome, resequencing, and metabolomes reveal the genetic background and molecular basis of the nutritional and medicinal properties of loquat (Eriobotrya japonica (Thunb.) Lindl). Horticulture Research, 8, 231.

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Intracellular Arginine Quantification by HPLC-MS

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For the determination of the intracellular content of arginine, cells were rapidly washed with PBS and the intracellular pool, extracted with a 10 min-incubation in acetonitrile/water (1:1) at 4°C, was analyzed by HPLC-ESI-MS/MS as previously described (27 (link)), with minor modifications. Briefly, HPLC separation was carried out on Ascentis Express HILIC column (Supelco, Bellefonte, PA, USA) at 35°C. Analytes (injection volume corresponding to 10 µl) were chromatographically separated under optimized gradient; mass spectrometric analyses were carried out using an AB SCIEX 4500 Q-TRAP (AB Sciex, Foster City, CA, USA) with a Turbo Ion Spray probe in positive mode. The monitored transitions for l-arginine and ¹⁵N₂-Arginine (used as internal standard) were 174.739 m/z→70.031 m/z and 176.739 m/z→70.031 m/z, respectively. Protein content in each condition was determined using a modified Lowry procedure (28 (link)) and l-arginine content was expressed as nmol/mg of protein.

Rotoli B.M., Barilli A., Visigalli R., Ingoglia F., Milioli M., Di Lascia M., Riccardi B., Puccini P, & Dall’Asta V. (2018). Downregulation of SLC7A7 Triggers an Inflammatory Phenotype in Human Macrophages and Airway Epithelial Cells. Frontiers in Immunology, 9, 508.

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