C57BL/6J mice were obtained from CLEA Japan, Inc. Sema6d−/− mice were generated as previously described (Takamatsu et al, 2010 (link)). B6.SJL-Ptprca Pepcb/BoyJ (CD45.1, #002014; Jax) mice were obtained from Jackson Laboratory. All mice were in the C57BL/6J background. 7- to 12-wk-old mice were used, and mouse experiments were randomized. For BM reconstitution, 1 × 107 total BM cells were intravenously transferred to recipient mice that had been lethally irradiated with a single dose of 10 Gy. Mice were then used for experiments at least 8 wk after reconstitution. All mice used in this study were housed in a specific pathogen–free facility. All protocols were approved by the Animal Research Committee of the Immunology Frontier Research Center (Osaka University).
C57bl 6j mice
Manufactured by CLEA Japan
Sourced in Japan
C57BL/6J mice are a widely used inbred mouse strain. They are a common model organism for biomedical research.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6j mice, by Oriental Yeast (3 mentions)
D12492, by Research Diets (3 mentions)
C57bl 6j, by CLEA Japan (2 mentions)
Type nmf, by Oriental Yeast (2 mentions)
B6.129p2 ccr5tmlkuz j, by Jackson ImmunoResearch (2 mentions)
Balb c nu nu mice, by CLEA Japan (2 mentions)
Crf 1, by Oriental Yeast (2 mentions)
Hfd32, by CLEA Japan (2 mentions)
Crf 1, by Charles River Laboratories (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
B27 supplement, by Thermo Fisher Scientific (2 mentions)
Microsprayer, by Penn-Century (1 mentions)
Xylazine, by Bayer (1 mentions)
Phenylephrine, by Santen (1 mentions)
Tropicamide, by Santen (1 mentions)
Ketamine hydrochloride, by Daiichi Sankyo (1 mentions)
Halofuginone, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
A06071302, by Research Diets (1 mentions)
Nod shijcl, by CLEA Japan (1 mentions)
174 protocols using c57bl 6j mice
1
Sema6d Knockout Mice for Bone Marrow Reconstitution
2
Dietary Effects on Mice Metabolism
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All animal procedures were performed in accordance with the Animal Experiment Guidelines of the Tohoku University, and the animal protocol was approved by the Animal Use Committee at the Tohoku University (2016AgA-009) [15 (link),16 (link)]. Male C57BL/6J mice (9-weeks-old) were obtained from Clea Japan (Tokyo, Japan). Mice had access to respective diets and distilled water ad libitum in a temperature- and humidity-controlled room with a 12/12-h light/dark cycle. After acclimatization to the normal diet for one week, 50 mice were randomly divided into five groups. Each group comprised 10 mice, divided into two cages. Mice were fed test diets for eight weeks. At 18 weeks of age, after a 12-hour fast, mice were weighed, and blood samples were collected after decapitation. To obtain serum, blood was centrifuged (900× g, 5 °C, 15 min). Brain, heart, lung, liver, pancreas, spleen, kidney, white adipose, and cecum tissues were removed and weighed. Serum and organs were stored at −80 °C until use.
3
High-Fat Diet and PHD Inhibitor Effects
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C57BL/6J mice were used (CLEA Japan, Inc. Tokyo, Japan). After 1 week of acclimatization, male C57BL/6J mice aged 8 weeks were randomly divided into (1) normal diet (ND; 3590 kcal/kg) (n = 3), ( 2) HFD (5062 kcal/kg, 62.2% kcal fat. [Oriental Yeast, Tokyo, Japan]) (n = 7) and 3) HFD with PHD inhibitor, JTZ-951 (provided from Japan Tobacco inc., Osaka, Japan), 0.005% mixed in chow (n = 7) groups.
Mice were fed for up to 20 weeks. Body weight (BW) was measured at baseline and every 4 weeks. Mice were housed in metabolic cages for 24 h for collection of urine at 20 weeks. After collecting urine at 20 weeks, mice were euthanized, blood was taken by cardiac puncture, and the kidneys, livers, and epididymal WAT were removed for tissue analyses 6 h after starvation.
The animals were housed in a temperature-and lightcontrolled environment and were allowed free access to chow and water, unless otherwise indicated. All animal experiments were approved by the ethics committee of the Graduate School of Medicine (P16-011, P16-257), the University of Tokyo, and performed in accordance with the guideline established by the committee.
Mice were fed for up to 20 weeks. Body weight (BW) was measured at baseline and every 4 weeks. Mice were housed in metabolic cages for 24 h for collection of urine at 20 weeks. After collecting urine at 20 weeks, mice were euthanized, blood was taken by cardiac puncture, and the kidneys, livers, and epididymal WAT were removed for tissue analyses 6 h after starvation.
The animals were housed in a temperature-and lightcontrolled environment and were allowed free access to chow and water, unless otherwise indicated. All animal experiments were approved by the ethics committee of the Graduate School of Medicine (P16-011, P16-257), the University of Tokyo, and performed in accordance with the guideline established by the committee.
4
Dietary Impact on Murine Metabolism
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2.1. Animals Animal feeding protocols have been summarized in Supplementary Fig. 1.
Seven-week-old male C57BL/6J mice were purchased from Clea Japan, Inc. (Tokyo, Japan). Since 8 weeks old, they were given free access to either a standard chow (SC) or HF diet for 2, 4 or 7 days. Gck +/-and Irs2-knockout (Irs2 -/-) mice were generated as described elsewhere [22, 23] .
We backcrossed these mice with C57BL/6J mice more than ten times. Male littermates derived from the intercrosses were fed an SC diet until 8 weeks of age and then were given free access to either the SC diet or an HF diet for 1 week. Additionally, the same experiment was performed using 48-week-old male C57BL/6J mice. All mice were housed on a 12-h light-dark cycle and maintained in accordance with standard animal care procedures based on institutional guidelines.
Seven-week-old male C57BL/6J mice were purchased from Clea Japan, Inc. (Tokyo, Japan). Since 8 weeks old, they were given free access to either a standard chow (SC) or HF diet for 2, 4 or 7 days. Gck +/-and Irs2-knockout (Irs2 -/-) mice were generated as described elsewhere [22, 23] .
We backcrossed these mice with C57BL/6J mice more than ten times. Male littermates derived from the intercrosses were fed an SC diet until 8 weeks of age and then were given free access to either the SC diet or an HF diet for 1 week. Additionally, the same experiment was performed using 48-week-old male C57BL/6J mice. All mice were housed on a 12-h light-dark cycle and maintained in accordance with standard animal care procedures based on institutional guidelines.
5
Evaluation of Antioxidant Therapies in Murine Pulmonary Fibrosis
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We purchased C57BL/6J mice from CLEA Japan, Inc. (Tokyo, Japan) and Keap1-based oxidative stress detector 48-transgenic (OKD48-Tg) and IL-1β-based dual-operating luciferase transgenic (IDOL-Tg) albino C57BL/6J mice from TransGenic Inc. (Kobe, Japan) [24 (link),25 (link)]. Only 12-week-old male mice were used in the experiment. However, the sex of mice was not expected to affect the results. Mice were intratracheally treated with BLM (20 mg/kg) using a microsprayer (Penn-Century, Philadelphia, PA, USA). TA293 (10 mg/kg) or mitoTA293 (10 mg/kg) was sprayed intratracheally using a microsprayer once a day from day 0 to day 7 (n = 5 in each group).
The animal studies in this study were strictly in compliance with the Guide for the Care and Use of Laboratory Animals by the US National Institutes of Health (NIH Publications No. 8023, revised 1978), and were approved by the Animal Experiment Ethics Committee of Takasaki University of Health and Welfare, Japan.
The animal studies in this study were strictly in compliance with the Guide for the Care and Use of Laboratory Animals by the US National Institutes of Health (NIH Publications No. 8023, revised 1978), and were approved by the Animal Experiment Ethics Committee of Takasaki University of Health and Welfare, Japan.
6
Murine Model of Ophthalmic Research
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All animal experiments followed the guidelines of the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the Animal Care Committee of Kansai Medical University (approval number: 17–013). Wild-type (WT) C57BL/6J mice were purchased from CLEA Japan (Tokyo, Japan). A total of 10 female mice aged 6 weeks were used for this study. All mice were kept in pathogen-free plastic cages with 12 hour light-dark cycles and had continuous free access to water and food. All plastic cages, water and bedding feed were purified before use. For all procedures, anesthetization was achieved by intraperitoneal injection of 90 mg/kg ketamine hydrochloride (Daiichi Sankyo Co., Tokyo, Japan) and 40 mg/kg xylazine (Bayer, Berlin, Germany); pupils were dilated with topical 0.5% tropicamide and 0.5% phenylephrine (Santen Pharmaceutical, Osaka, Japan).
7
Standardized Housing for C57BL/6J Mice
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C57BL/6J mice were obtained from Clea Japan (Tokyo, Japan), and were housed at Fukushima Medical University School of Medicine under a standard 12-h light/12-h dark schedule (lights on at 7:00 a.m.). Food and water were available ad libitum unless otherwise stated. All animal experiments were performed in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals, and were approved by our university’s committee for animal experimentation.
8
Halofuginone Therapeutic Effects in Mice
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All procedures related to animal experiments were approved by the Institutional Animal Care and Use Committee of Keio University, and were in accordance with the National Institutes of Health (NIH) guidelines for work with laboratory animals, the Association for Research in Vision and Ophthalmology (ARVO) statement for the Use of Animals in Ophthalmic and Vision Research, and Animal Research: Reporting of in Vivo Experiments (ARRIVE) guidelines. All experiments were performed with 8-weeks-old male C57/BL6J mice (CLEA Japan, Yokohama, Japan). Animals were divided into two groups randomly in the completely blind manner and intraperitoneally injected with phosphate buffered saline (PBS) or halofuginone dissolved in PBS (0.2 mg/kg, Sigma-Aldrich, USA) once per day for 7 days. All mice were bred with a standard rodent diet (MF, Oriental Yeast Co., Ltd., Japan) and given free access to water. All cages were maintained under controlled lighting conditions (12 h light/12 h dark).
9
Genetically Modified Mouse Models
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Seven- to ten-week-old female C57BL/6J mice were purchased from CLEA Japan, Inc. (Osaka, Japan). MyD88 KO mice were purchased from Oriental BioService, Inc. (Kyoto, Japan). IL-12p40 KO and STING mutant mice (Tmem173gt), which have a loss-of-function mutation at the ligand-binding site of STING 46 (link), were purchased from Jackson Laboratories (Bar Harbor, ME, USA). IRF3/7 DKO mice were generated from IRF3 KO 22 and IRF7 KO mice, the latter of which was provided by the RIKEN BRC (Ibaraki, Japan) via the National Bio-Resource Project of the MEXT, Japan 47 (link). IFNAR2 KO mice were obtained from B&K Universal. All of the animal experiments were conducted according to the guidelines of the Animal Care and Use Committee of RIMD and IFReC of Osaka University, and the use of animals was approved by Osaka University.
10
Murine Two-Cell Embryo Transfer
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Male (10 to 18 weeks old) and female (8 to 13 weeks old) C57BL/6J mice (CLEA Japan, Inc.,
Tokyo, Japan) were used as sperm and oocytes donors, respectively. Twelve-week-old female
ICR mice (CLEA Japan, Inc.) were used as recipients of the two-cell embryo transfer. The
mice were housed in a specific pathogen-free room with a 12-h dark/light cycle
(illumination from 7:00 to 19:00) at 22 ± 1°C and provided food and water ad
libitum. The Animal Care and Use Committee of Kumamoto University School of
Medicine approved the protocols for all animal experiments (ID: A2019-026), and all
methods were performed in accordance with the relevant guidelines and regulations.
Tokyo, Japan) were used as sperm and oocytes donors, respectively. Twelve-week-old female
ICR mice (CLEA Japan, Inc.) were used as recipients of the two-cell embryo transfer. The
mice were housed in a specific pathogen-free room with a 12-h dark/light cycle
(illumination from 7:00 to 19:00) at 22 ± 1°C and provided food and water ad
libitum. The Animal Care and Use Committee of Kumamoto University School of
Medicine approved the protocols for all animal experiments (ID: A2019-026), and all
methods were performed in accordance with the relevant guidelines and regulations.
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