Log In Sign up
The largest database of trusted experimental protocols

F3777

Manufactured by Bio-Rad
Visit Supplier

The F3777 is a lab equipment product manufactured by Bio-Rad. It is a device used for scientific applications, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Ab10135, by Abcam (2 mentions) Donkey secondary antibodies, by Jackson ImmunoResearch (2 mentions) Mca519g, by Abcam (2 mentions) Bt 562, by Abcam (2 mentions) Eclipse 80i microscope, by Nikon (2 mentions) Ab6640, by Biomedical Technologies (2 mentions)

2 protocols using f3777

1

Multimodal Vascular Tissue Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry, perfused aortas were post-fixed in 4% PFA overnight, then cryosectioned at 8 μm and stained with primary antibodies recognizing PECAM (BD Bioscience 553370, 1:100), αSMA (Sigma F3777, 1:250), MOMA-2 (AbD Serotec MCA519G, 1:100), F4/80 (Abcam ab6640, 1:100), SMMHC (Biomedical Technologies Inc. BT-562, 1:100) or Sm22α (Abcam ab10135, 1:100), with appropriate donkey secondary antibodies (Jackson ImmunoResearch). Immunofluorescence microscopy was performed with a Nikon Eclipse 80i microscope connected to a digital camera. Final image processing was performed with Photoshop (Adobe). For histological stains, perfused aortas were post-fixed with Bouin’s solution, paraffin embedded, and sectioned at 5 μm before staining with Movat’s pentachrome, Masson’s trichrome, or Prussian blue. Formalin-fixed aortic roots were cryosectioned at 10 μm and stained with Oil red O.
He C., Medley S.C., Hu T., Hinsdale M.E., Lupu F., Virmani R, & Olson L.E. (2015). PDGFRβ signaling regulates local inflammation and synergizes with hypercholesterolemia to promote atherosclerosis. Nature communications, 6, 7770.
+ Open protocol
+ Expand
2

Multimodal Vascular Tissue Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry, perfused aortas were post-fixed in 4% PFA overnight, then cryosectioned at 8 μm and stained with primary antibodies recognizing PECAM (BD Bioscience 553370, 1:100), αSMA (Sigma F3777, 1:250), MOMA-2 (AbD Serotec MCA519G, 1:100), F4/80 (Abcam ab6640, 1:100), SMMHC (Biomedical Technologies Inc. BT-562, 1:100) or Sm22α (Abcam ab10135, 1:100), with appropriate donkey secondary antibodies (Jackson ImmunoResearch). Immunofluorescence microscopy was performed with a Nikon Eclipse 80i microscope connected to a digital camera. Final image processing was performed with Photoshop (Adobe). For histological stains, perfused aortas were post-fixed with Bouin’s solution, paraffin embedded, and sectioned at 5 μm before staining with Movat’s pentachrome, Masson’s trichrome, or Prussian blue. Formalin-fixed aortic roots were cryosectioned at 10 μm and stained with Oil red O.
He C., Medley S.C., Hu T., Hinsdale M.E., Lupu F., Virmani R, & Olson L.E. (2015). PDGFRβ signaling regulates local inflammation and synergizes with hypercholesterolemia to promote atherosclerosis. Nature communications, 6, 7770.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!