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9 protocols using pluronic p105

1

Targeted Drug Delivery for Lung Cancer

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Ax was purchased from Boehringer-Ingelheim Co., Ltd (Ingelheim, Germany). PTX was purchased from Dalian Meilun Biotech Co., Ltd (Dalian, China). PEG2000-PLA2000 was provided by East China University of Science and Technology. Pluronic P105 was brought from BASF, Ludwigshafen (Germany). DiR was obtained from Sigma-Aldrich (Saint Louis, USA), A549 lung cancer cells and A549-luciferase lung cancer cells were purchased from Shanghai cell bank, Chinese Academy of Sciences (China). ICR mice, BALB/c nude mice and SD rats were purchased from the animal experiment center of Fudan University.
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2

Murine Macrophage Cell Culture Protocol

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All chemicals and cell culture reagents were obtained from commercial sources and used without purification. Celecoxib was purchased from LC Laboratories, Woburn, MA, USA. Perfluoropolyethylene glycol ether (PFPE) was obtained as a generous gift from Celsense Inc., Pittsburgh, PA, USA. Pluronic® P105 was obtained from BASF, Cremophor EL® (CrEL) was obtained from Sigma Aldrich. 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine (DiD) dye was purchased from Life Technologies. Bacterial lipopolysaccharide (LPS) and cytochalasin B were obtained from Sigma Aldrich. The CellTiter-Glo® cell viability assay kit and Griess Reagent System for nitrite measurements were purchased from Promega, Madison, WI, USA. Phycoerythrin conjugated Rat anti-mouse CD86 (CD86-PE) antibody and Rat IgG2a,k conjugated to PE (isotype control) were obtained from BD Biosciences, San Jose, CA. The murine macrophage cell line RAW 264.7 (TIB-71) was obtained from ATCC. Cells were culture as previously reported [21 (link)]. Unless specified, cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2.
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3

Synthesis of Acid-Degradable Cyclodextrin-Based Pseudorotaxanes

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Acid-degradable PRXs composed of N3-β-CD as a cyclic molecule, PEG-b-PPG-b-PEG (Pluronic P105; BASF, Ludwigshafen, Germany) as an axial polymer, and N-Trt groups as acid-labile stopper molecule (N3-PRX) were synthesized as previously described [39 (link)]. In this study, N3-PRX with 14.9 threaded N3-β-CDs and a number-average molecular weight of 24,300 was used for the synthesis of cRGD-Me-PRXs. Similarly, acid-degradable PRX with 10.5 threaded β-CDs and a number-average molecular weight of 19,000 was utilized for the synthesis of control Me-PRX. Butynyl cRGDfK and cRGEfK peptides were synthesized and characterized as described in Supporting Information Figures S1 and S2. cRGD-modified Me-β-CD (cRGD-Me-β-CD) was prepared by degrading cRGD-Me-PRX as previously reported procedure [42 (link)]. Randomly methylated β-CD (Me-β-CD) and sodium ascorbate were obtained from Merck KGaA (Darmstadt, Germany). Methyl iodide (MeI), copper (II) sulfate pentahydrate, and sodium N,N-diethyldithiocarbamate trihydrate (DDC) were obtained from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). All other reagents and solvents were obtained from Fujifilm Wako Pure Chemical and Kanto Chemical (Tokyo, Japan).
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4

Formulation Development and Characterization

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Pluronic® P105 was obtained from BASF Corporation. Cremophor EL (CrEL) was purchased from Sigma Aldrich. Miglyol 812N, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and the Raw 264.7 cell line were purchased from ATCC. Prostaglandin E2 EIA Kit-monoclonal was purchased from Cayman Chemical Company. DiR dye was from Life Technologies. Perfluoro(polyethylene glycol dimethyl ether) or PFPE oxide (CF3O(CF2CF2O)nCF3, where n=8–13) was obtained from Exfluor Research Corporation.
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5

Macrophage Cytotoxicity Evaluation Protocol

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Cremophor EL® and bacterial lipopolysaccharide (LPS) were obtained from Sigma Aldrich. Pluronic® P105 was obtained from BASF. 1,1’-Dioctadecy-3,3,3’,3’ Tetramethylindotricarbocyanine Iodide (DiR dye) was purchased from ThermoFisher Scientific. The CellTiter-Glo® cell viability assay kit was purchased from Promega, Madison, WI, USA. The murine macrophage cell line RAW 264.7 (TIB-71) was obtained from ATCC Lot #61524889 and cultured in 37 °C cell culture hood in a humidified atmosphere containing 5% C02.
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6

Synthesis and Characterization of Lipid Nanoparticles

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Perfluoro-15-crown-5-ether (PCE) and perfluoropolyether (PFPE) dimethyl ester were purchased from Exfluor Research Corporation (Round Rock, TX). PFPE oxide was a gift from Celsense Inc. (Pittsburgh, PA). Kolliphor® EL (CrEL) was purchased from Sigma Aldrich. Pluronic® P105 was purchased from BASF Corp. Super Refined® Olive Oil NF-LQ (MH) was a gift from CRODA, Inc. (NJ, USA). Miglyol 812N was purchased from Sasol. Transcutol (2-(2-Ethoxyethoxy)ethanol) was purchased from Spectrum Chemicals (NJ, USA). Tyramine was purchased from Aldrich. Dulbecco’s modified Eagle medium (DMEM) (Mediatech Inc., Manassas, VA) was used to simulate biological conditions. All reagents were used as received without further purification. Deionized (DI) water was used. (Suppl. Information Table 1)
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7

Formulation of Fluorinated Nanocarriers

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Pluronic P-105 was purchased from BASF (Florham Park, New Jersey, USA). Pluronic P-123 was purchased from Millipore Sigma (St. Louis, Missouri). Perfluorooctyl bromide and perfluoro-15-crown-5-ether were purchased from Exfluor Research Corp (Round Rock, Texas, USA). Miglyol 812 (capric acid/caprylic acid triglycerides, known as medium chain triglycerides, MCT) was obtained from CREMER Oleo (Hamburg, Germany). Transcutol was purchased from Spectrum Chemical Mfg Corp (New Brunswick, New Jersey, USA). Trifluoroacetic acid and deuterium oxide, 99.8% were obtained from Acros Organics (Thermo Fisher Scientific, Waltham, Massachusetts, U.S.). Dulbecco’s Modified Eagle’s Medium was supplied by Corning (Corning, New York, U.S.), and fetal bovine serum was supplied by ATCC (Manassas, Virginia). All chemicals and reagents were used without modification.
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8

Formulation and Characterization of Pluronic-Based Nanoemulsions

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Pluronic® P105 was purchased from BASF Corporation (Florham Park, NJ, USA). Pluronic P123 and chremophor EL (ethoxylated castor oil) were obtained from Sigma Aldrich (St. Louis, MO, USA). Olive oil was a kind gift from Croda International Plc (Snaith, UK). Campul® PG-8 NF was from Abitec (Columbus, OH, USA). Perfluoro(polyethylene glycol dimethyl ether), referred to as PFPE, (CF3O(CF2CF2O)nCF3, where n = 8–13) was obtained from Exfluor Research Corporation (Round Rock, TX, USA). Dulbecco’s modified eagle medium (DMEM; GIBCO-BRL, Rockville, MD. USA). All reagents were used without further purification and the water that we used was deionized (DI).
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9

Perfluorochemical Compounds for Biomedical Applications

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Perfluorooctyl bromide (PFOB) was purchased from Exfluor, catalog number C8BR. Perfluorodecalin (PFD) was purchased from Sigma-Aldrich (Saint Louis, MO, USA), catalog number P9900-25G. Perfluoro(polyethylene glycol dimethyl ether) oxide (PFPE) was generously donated by Celsense, Inc., Pittsburgh, PA, USA. Perfluro-15-crown-5- ether (PCE) was purchased from Exfluor Research Company (Round Rock, TX, USA), product number F15CROWN5. Transcutol (2-(2-ethyoxyethyoxy)-ethanol (catalog E1022) and olive oil (catalog OL130) were purchased from Spectrum. Miglyol 812 was generously donated by Cremer Oleo (Hamburg, Germany), product number 6330. DMSO (dimethyl sulfoxide) was purchased from Acros Organics, catalog number 414885000. Fluorescent dyes were purchased from Invitrogen (Waltham, MA, USA). Catalog numbers for DiD (DiIC18(5), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate), DiR (DiIC18(7), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide), and DiI (DiIC18(3), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) were D307, D12731, and D282, respectively. Cremophor EL was purchased from Sigma-Aldrich, catalog number C5135. Pluronic P105 was obtained from BASF Corporation (Florham Park, NJ, USA), material number 51207527.
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