Mhcii 1 a 1 e

Manufactured by BioLegend

The MHCII (I-A/I-E) is a lab equipment product that serves as a major histocompatibility complex (MHC) class II molecule. It is involved in the presentation of peptide antigens to T helper cells.

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Lab products found in correlation

Cd11c, by BioLegend (3 mentions) Cd11b, by BioLegend (3 mentions) Mpdca1, by BioLegend (2 mentions) Live dead fixable near ir dead cell staining kit, by Thermo Fisher Scientific (2 mentions) Siglec h, by BioLegend (2 mentions) Anti mouse cd11c, by BioLegend (2 mentions) Pe conjugated anti mouse ly49q, by MBL Life Science (2 mentions) Ter119, by BioLegend (1 mentions) Cd138, by BD (1 mentions) Streptavidin, by Thermo Fisher Scientific (1 mentions) Cxcr5, by BD (1 mentions) Il 12p40, by BioLegend (1 mentions) Cd49b, by Thermo Fisher Scientific (1 mentions) P nitrophenyl phosphate tablets, by Merck Group (1 mentions) 3 amino 9 ethylcarbazole tablets, by Merck Group (1 mentions) Pna biotin, by Merck Group (1 mentions) Cd45ra, by BD (1 mentions) Lipopolysaccharide, by Merck Group (1 mentions) Brefeldin a, by LC Laboratories (1 mentions) Annexin 5, by BD (1 mentions)

6 protocols using mhcii 1 a 1 e

Multi-parameter Flow Cytometric Analysis of Immune Cell Subsets

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Abs specific for CD19, CD3e, CD4, PD-1, APRIL, CD11c, IL-12p40, Ter119, CD11c, MHCII IA/IE, TACI were purchased from Biolegend; GL7, FAS, CXCR5, CD138, B220, IgG, Siglec, CD19, CD8, CD45RA, Annexin V, and Streptavidin-HRP from BD Biosciences; GR-1, CXCR4, CD3e, CD49b, CD40, CD86, CD80 from eBiosciences; BCMA from R&D Systems. IgM (B7.6) and anti-Fc (2.4G2) were purified from hybridomas. Live/Dead Pacific Orange, Fixable Blue dyes, anti-IgG-Alkaline Phosphatase, anti-IgM- Alkaline Phosphatase, Streptavidin were purchased from Invitrogen/Life Technologies; SDF-1 from Shennendoah Biotechnology; anti-IgG2- Alkaline Phosphatase from Southern Biotech; p-Nitrophenyl Phosphate tablets, 3-amino-9-ethylcarbazole tablets, PNA-biotin, and lipopolysaccharide from Sigma; anti-IgGFc from Jackson Immunoresearch; Brefeldin A from LC Laboratories. IsdB peptide (AA 40-613) was a gift from Dr. Benfang Lei (Montana State University). Pokeweed mitogen was a gift from Dr. Shane Crotty (La Jolla Institute for Allergy & Immunology).

Keener A.B., Thurlow L.T., Kang S., Spidale N.A., Clarke S.H., Cunnion K.M., Tisch R., Richardson A.R, & Vilen B.J. (2016). Staphylococcus aureus Protein A Disrupts Immunity Mediated by Long-Lived Plasma Cells. Journal of immunology (Baltimore, Md. : 1950), 198(3), 1263-1273.

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Murine Neuroinflammation Immunostaining Protocol

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We obtained murine recombinant IL-33 (580506), AF647-conjugated Aβ (clone: 6E10) antibody (803021), APC-conjugated MHC-II (clone: M5/114.15.2) antibody (107614), FITC-conjugated VCAM1 (clone: MVCAM.A) antibody (105706) and MHC-II (I-A/I-E) (clone: M5/114.15.2) antibody (107601) from BioLegend. We obtained ICAM1-neutralizing (clone: YN1/1.7.4) antibody (BE0020)^{75 (link),76 (link)} and VCAM1-neutralizing (clone: M/K-2.7) antibody (BE0027)^{69 (link),77 (link)} from Bio X Cell. We obtained AF488-conjugated CD11b (clone: M1/70) antibody (53-0112-82), APC-conjugated CD11b (clone: M1/70) antibody (17-0112-83) and biotinylated CD11b (clone: M1/70) antibody (13-0112-82) from eBioscience. ApoE-neutralizing (clone: HJ6.3) antibody was a gift from D. Holtzman^{36 (link)}. We obtained DAPI (D3571) from Life Technologies, and mouse ITGB2 recombinant protein (LS-G14036-10) was from LSBio. We obtained mouse ApoE recombinant protein (MBS955382) from MyBioSource as well as CCR7 neutralizing (clone: 4B12) antibody (MAB3477)^{78 (link)} and VCAM1 antibody (BBA5) from R&D Systems. We obtained recombinant mouse CD44 protein (53953-M08H) from Sino Biological, MeX04 (4920) from Tocris Bioscience and Iba1 antibody (019-19741) from Wako.

Lau S.F., Wu W., Wong H.Y., Ouyang L., Qiao Y., Xu J., Lau J.H., Wong C., Jiang Y., Holtzman D.M., Fu A.K, & Ip N.Y. (2023). The VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology. Nature Aging, 3(10), 1219-1236.

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Interferon Signaling and Immune Cell Analysis

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Reagents were obtained from: Doxycycline, ClonTech (#8634-1); Jak Inhibitor I (#420099) and PS-1145 (P6624), Sigma. IFN α and β were from PBL Assay Science (#11410-2 and #11200-1), and IFNγ from PeproTech (#300-02). Lipofectamine 2000 was from Life Technologies. Flow antibodies/reagents were from Biolegend: Zombie UV (#423107), HA (#901526), IFNAR1 (#21370-3), CD11b (#101208), MHCII (I-A/I-E) (#107620), CD86 (#105036), CD11c (#117318), CD45 (#103116 or #103128), F4/80 (#123116), Ly6c (#128033), Ly6g (#127622), mouse Fc Trustain block (#101319), NK1.1 (#108724), anti-mouse CD69 (#104514) and CD25 (#102036). IFNGR (10338-MM05-A) was from Sino Biologic.

Henrich I.C., Jain K., Young R., Quick L., Lindsay J.M., Park D.H., Oliveira A.M., Blobel G.A, & Chou M.M. (2021). Ubiquitin-specific protease 6 functions as a tumor suppressor in Ewing Sarcoma through immune activation. Cancer research, 81(8), 2171-2183.

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Fluorescent Antibody-Based Immune Profiling

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Fluorescent-conjugated antibodies including anti-mouse CD11c, CD11b,
B220, SiglecH, mPDCA1, and MHCII (I-A/I-E) were purchased from Biolegend (San
Diego, CA). PE-conjugated anti-mouse Ly49Q was purchased from MBL International
(Woburn, MA). LIVE/DEAD fixable near-IR dead cell staining kit was purchased
from Life Technologies (Grand Island, NY).

Scott J.L., Wirth J.R., EuDaly J.G., Gilkeson G.S, & Cunningham M.A. (2016). Plasmacytoid dendritic cell distribution and maturation are altered in lupus prone mice prior to the onset of clinical disease. Clinical immunology (Orlando, Fla.), 175, 109-114.

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Comprehensive Immune Cell Phenotyping

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Fluorescent conjugated antibodies including anti-mouse CD11c, CD11b, B220, SiglecH, mPDCA1, PDC-TREM, MHC II (I-A/I-E), and CD40 were purchased from Biolegend (San Diego, CA). PE-conjugated anti-mouse Ly49Q was purchased from MBL International (Woburn, MA). LIVE/DEAD fixable near-IR dead cell staining kit was purchased from Life Technologies (Grand Island, NY). Mouse CpG DNA was purchased from Hycult Biotech (Canton, MA).

Scott J.L., Cunningham M.A., Naga O.S., Wirth J.R., EuDaly J.G, & Gilkeson G.S. (2015). Estrogen receptor alpha deficiency modulates TLR ligand mediated PDC-TREM expression in plasmacytoid dendritic cells in lupus prone mice. Journal of immunology (Baltimore, Md. : 1950), 195(12), 5561-5571.

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Multiparameter Flow Cytometry for Immune Cell Profiling

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For fluorescent staining, single cell suspensions were blocked with 5% rat serum for 5 min. Fluorochrome-conjugated monoclonal antibodies were used: CD103 (2E7), CD115 (AFS98), CD11b (M1/70), CD11c (N418), CD4 (RM4-5), CD45 (30-F11), CD45.1 (A20), CD45.2 (104), CD64 (X54-5/7.1), F4/80 (BM8), Ly6C (HK1.4), Ly6G (1A8), MHCII I-A/I-E (M5/114.15.2), TCRβ (H57-597), TCR Vα2 (B20.1), Tim4 (RMT4-54) (all from Biolegend), TCR Vβ5.1 (MR9-4, BD), SiglecF (E50-2440, BD). Cell viability was established with 7AAD (Biolegend) or Fixable Viability Dye eFluor™ 780 (eBioscience). For intracellular staining, the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) was used according to manufacturer’s instructions. FoxP3 (FJK-16 s, eBioscience) and Ki67 (SolA15, eBioscience) were used for intracellular staining. Flow cytometry was performed using a LSRFortessa (BD) and analysed with FlowJo software (FlowJo). Cell sorting was performed using a FACSAriaII (BD).

Guillaume J., Leufgen A., Hager F.T., Pabst O, & Cerovic V. (2023). MHCII expression on gut macrophages supports T cell homeostasis and is regulated by microbiota and ontogeny. Scientific Reports, 13, 1509.

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