Maxwell 16 lev blood dna kit

Manufactured by Promega

Sourced in United States, Germany, France, Japan

The Maxwell 16 LEV Blood DNA Kit is a laboratory instrument designed to automate the extraction and purification of genomic DNA from whole blood samples. It utilizes a magnetic bead-based technology to efficiently isolate high-quality DNA that can be used for various downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Maxwell 16 research, by Promega (6 mentions) Nanodrop 1000, by Thermo Fisher Scientific (6 mentions) Maxwell 16 system, by Promega (4 mentions) Quantstudio 12k flex real time pcr system, by Thermo Fisher Scientific (4 mentions) Maxwell 16 instrument, by Promega (4 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (4 mentions) Proteinase k, by Promega (3 mentions) Tissuelyser 2, by Qiagen (3 mentions) Peqgold trifast, by Avantor (3 mentions) Maxwell 16 machine, by Promega (3 mentions) Quantus fluorometer, by Promega (2 mentions) 5 mm stainless steel bead, by Qiagen (2 mentions) Nuclease free water, by Qiagen (2 mentions) Lysozyme, by Merck Group (2 mentions) Wizard genomic dna purification kit, by Promega (2 mentions) Thermomixer, by Eppendorf (2 mentions) Global screening array, by Illumina (2 mentions) Qiaamp dna micro kit, by Qiagen (2 mentions) Nanodrop nd 2000, by Thermo Fisher Scientific (2 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Maxwell 16 (79 citations) Blood DNA kit (4 citations) Maxwell kits (2 citations)

73 protocols using maxwell 16 lev blood dna kit

Extracting RNA and DNA from Uterus Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated from uterus tissue samples using the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega GmbH, Mannheim, Germany) according to the manufacturer’s instructions. The reverse transcription of messenger RNA (mRNA) into cDNA was performed using the GoScript Reverse Transcription System (Promega GmbH) according to the technical manual.
For extraction of genomic DNA from uterus tissue samples, we used the Maxwell 16 LEV Blood DNA Kit (Promega GmbH) with a modified lysis protocol (crushing a piece of tissue of ∼5 mm in diameter, incubation at 56° for 2 hr with 400 µl lysis buffer and 40 µl proteinase K). Extraction of genomic DNA from hair roots was also performed using the Maxwell 16 LEV Blood DNA Kit (Promega GmbH) and the same modified lysis protocol as described above, omitting the crushing step.

Lehner S., Ekhlasi-Hundrieser M., Detering C., Allerkamp H., Pfarrer C, & von Depka Prondzinski M. (2017). A 12.3-kb Duplication Within the VWF Gene in Pigs Affected by Von Willebrand Disease Type 3. G3: Genes|Genomes|Genetics, 8(2), 577-585.

+ Open protocol

+ Expand

Automated DNA Extraction from Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted using the Maxwell 16 LEV Blood DNA kit (Promega) and the automated Maxwell 16 device (AS1000; Promega) per the manufacturer's instructions. In each extraction batch of 15 samples, a negative extraction control (NEC; lysis buffer only) was included.

van Henten S., Kassa M., Fikre H., Melkamu R., Mekonnen T., Dessie D., Mulaw T., Bogale T., Engidaw A., Yeshanew A., Cnops L., Vogt F., Moons K.G., van Griensven J, & Pareyn M. (2024). Evaluation of Less Invasive Sampling Tools for the Diagnosis of Cutaneous Leishmaniasis. Open Forum Infectious Diseases, 11(4), ofae113.

+ Open protocol

+ Expand

Pharmacogenomic Genotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Buffy coats were prepared from 9 mL whole‐blood EDTA samples after plasma separation. Genomic DNA was extracted from the buffy coats using the Maxwell 16 LEV Blood DNA Kit on a Maxwell 16 Research automated nucleic acid extraction system (Promega, Madison, WI). The participants were genotyped for the CYP2C8*2 (rs11572103), *3 (rs10509681 and rs11572080), and *4 (rs1058930), CYP2C19*2 (rs4244285), *3 (rs4986893), *8 (rs41291556), and *17 (rs12248560), UGT1A3*2 (rs3821242 and rs6431625), *3 (rs3821242), and *6 (rs3821242, rs6431625, and rs45449995), and CES1 c.428G>A (rs71647871) alleles with commercially available or custom TaqMan assays with OpenArray technology on a QuantStudio 12K Flex real‐time polymerase chain reaction system (Life Technologies, Carlsbad, CA).

Itkonen M.K., Tornio A., Filppula A.M., Neuvonen M., Neuvonen P.J., Niemi M, & Backman J.T. (2017). Clopidogrel but Not Prasugrel Significantly Inhibits the CYP2C8‐Mediated Metabolism of Montelukast in Humans. Clinical Pharmacology and Therapeutics, 104(3), 495-504.

+ Open protocol

+ Expand

Whole-Genome Sequencing of Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole‐genome sequencing (WGS) was conducted on DNA sample extracted from whole leukocytes of peripheral blood obtained from the patient before imatinib therapy. Genomic DNA was extracted from the whole blood using Maxwell^® 16 LEV Blood DNA Kit (Promega, Fitchburg, WI), sheared into approximately 350 bp fragments, and used to make a library with TruSeq Nano DNA Sample Prep Kit (Illumina, San Diego, CA). Sequencing was performed on an Illumina HiSeq X platform in paired‐end 150 bp configuration.

Iriyama N., Takahashi H., Naruse H., Miura K., Uchino Y., Nakagawa M., Iizuka K., Hamada T., Hatta Y., Nakayama T, & Takei M. (2019). A novel fusion gene involving PDGFRB and GCC2 in a chronic eosinophilic leukemia patient harboring t(2;5)(q37;q31). Molecular Genetics & Genomic Medicine, 7(4), e00591.

+ Open protocol

+ Expand

Sensitive Skin Microbiome Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The first D-Squame Skin Stripping disc in 300 μL 1X DNA/RNA shield was subjected to extraction with the Maxwell 16 LEV Blood DNA kit (Promega, Netherlands) as specified in the manufacturer’s manual using the automated Maxwell 16 Instrument (AS1000, Promega). Nucleic acids were subsequently tested by qPCR targeting kinetoplast DNA (kDNA) as described by Merdekios et al. (17 (link)). The specific primers and probes used were LC-F (5’-TATTTTACACCAACCCCCAGT-3′), LC-R (5’-GGTAGGGGCGTTCTGC-3′) and a FAM-labeled LC-probe (5’-CAGAAAYCCCGTTCAAAAAATGGC-3′). If a sample was negative, the second D-Squame Skin Stripping disc was tested. If there was still no fluorescence, an HBB PCR was performed as described by Steinau et al. (18 (link)) to assess if there was sufficient tissue on the discs and whether the DNA extraction was conducted successfully.

van Henten S., Pareyn M., Tadesse D., Kassa M., Techane M., Kinfe E., Girma N., Demeke D., Mesay M., Kassa M., Temesgen R., Shewangizaw M., Massebo F., van Griensven J., Wegayehu T, & Merdekios B. (2023). Community-based treatment of cutaneous leishmaniasis using cryotherapy and miltefosine in Southwest Ethiopia: the way forward?. Frontiers in Medicine, 10, 1196063.

+ Open protocol

+ Expand

Salmonella Agona Genomic DNA Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Salmonella Agona isolates were freshly grown on blood agar plates. One inoculation loop of bacterial material was suspended in 50 µL phosphate buffered saline (PBS) and cells were pre-treated with 1 µg lysozyme for 15 min at 37 °C followed by a 2 hour incubation step at 65 °C with 200 µL incorporation buffer, 200 µL lysis buffer, 30 µL 20 mg/mL Proteinase K and 10 µL 10 mg/mL ribonuclease (RNase) A (all reagents from Promega, Mannheim, Germany). Genomic DNA (gDNA) was then isolated with the Maxwell 16 LEV Blood DNA Kit on the Maxwell 16 instrument (Promega, Mannheim, Germany) according to manufacturer’s instructions with Tris buffer for gDNA elution.
Whole genome libraries for NGS were prepared using the Nextera XT kit (Illumina, San Diego, California, US). Next generation sequencing was performed on the Illumina MiSeq with 2x250 bp paired-end reads. Sequencing runs were evaluated for quality using the Illumina SAV Software.
Sequencing data were uploaded to the NCBI sequence read archive (SRA) [26 ], under BioProject PRJNA473689.

Dangel A., Berger A., Messelhäußer U., Konrad R., Hörmansdorfer S., Ackermann N, & Sing A. (2019). Genetic diversity and delineation of Salmonella Agona outbreak strains by next generation sequencing, Bavaria, Germany, 1993 to 2018. Eurosurveillance, 24(18), 1800303.

+ Open protocol

+ Expand

DNA and RNA Extraction from Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA extraction was performed with the Maxwell^® 16 LEV Blood DNA Kit (Promega, Charbonnières-les-Bains, France) on EDTA blood samples according to the manufacturer’s instructions. RNA was directly extracted from 5 mL of PAXgene^TM blood samples with the PAXgene^TM Blood RNA System (Qiagen, Courtaboeuf, France) according to the manufacturer’s instructions. DNA concentrations were quantified using a NanoDrop 1000 Spectrophotometer v3.8 (Thermo Fisher Scientific, Courtaboeuf, France).

Pacot L., Burin des Roziers C., Laurendeau I., Briand-Suleau A., Coustier A., Mayard T., Tlemsani C., Faivre L., Thomas Q., Rodriguez D., Blesson S., Dollfus H., Muller Y.G., Parfait B., Vidaud M., Gilbert-Dussardier B., Yardin C., Dauriat B., Derancourt C., Vidaud D, & Pasmant E. (2019). One NF1 Mutation may Conceal Another. Genes, 10(9), 633.

+ Open protocol

+ Expand

HLA Typing of Glioma Patient Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was isolated from patient blood and glioma tissue using the NucleoSpin Tissue Kit (Macherey-Nagel) or the Maxwell® 16 LEV Blood DNA Kit (Promega). The isolated DNA was HLA-typed as described previously.^{24 (link)}

Lu K.H., Michel J., Kilian M., Aslan K., Qi H., Kehl N., Jung S., Sanghvi K., Lindner K., Zhang X.W., Green E.W., Poschke I., Ratliff M., Bunse T., Sahm F., von Deimling A., Wick W., Platten M, & Bunse L. (2022). T cell receptor dynamic and transcriptional determinants of T cell expansion in glioma-infiltrating T cells. Neuro-Oncology Advances, 4(1), vdac140.

+ Open protocol

+ Expand

Plasma gDNA Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

gDNA was extracted from plasma samples using Maxwell 16 LEV Blood DNA Kit (Promega, Madison, WI, #AS1290). Concentrations were measured using Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA) or Quantus Fluorometer (Promega) calibrated with double stranded DNA (ThermoFisher Scientific). Samples with concentrations below 0.3 ng/μl, or that were cloudy were amplified and repaired using the whole genome amplification REPLI-g FFPE Kit (Qiagen, Germantown, MD).

Boullerne A.I., Wallin M.T., Culpepper W.J., Maloni H., Boots E.A., Sweeney D.M, & Feinstein D.L. (2021). Liver kinase B1 rs9282860 polymorphism and risk for multiple sclerosis in White and Black Americans. Multiple sclerosis and related disorders, 55, 103185.

+ Open protocol

+ Expand

Quantifying Lentiviral Vector Persistence

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, 1 million mouse splenocytes were harvested and genomic DNA extracted by ACGT, Inc. using the Promega Maxwell® 16 LEV Blood DNA Kit. To determine CAR-T persistence, a unique fragment (c-frag) contained within the lentiviral vector backbone of anti-HIV CARs, but not the HIV-1 virus, was used to assess copy numbers per 50 ng of genomic DNA isolated from mouse splenocytes. The following primers and probe were used to detect c-frag: Forward primer: 5’GGAGTTGAGACCAGTGTAGT-3’, Reverse primer: 5’-CCACTCCTGACAACTACTCT-3’, Probe: 5’-FAM-CAGTAGGTGAAGGAGTCGTAGTTG-TAMRA-3’. The following primers and probe were used to detect the polypyrimidine tract binding protein 2 (PTBP2) gene to control for PCR inhibition: Forward primer: 5’-TCTCCATTCCCTATGTTCATGC-3’, Reverse primer: 5’-GTTCCCGCAGAATGGTGAGGTG-3’, Probe: 5’-JOE-ATGTTCCTCGGACCAACTTG-BHQ-1-3’. The PTBP2 gene was used as an internal control to assess PCR inhibition. The number of cFrag copies in splenic DNA was calculated using a standard curve consisting of known quantities of a plasmid DNA containing the c-frag genetic tag followed by normalization to input splenic DNA (0.05 μg) multiplied by the volume of the reaction added per well (12.5 μL).

Anthony-Gonda K., Bardhi A., Ray A., Flerin N., Li M., Chen W., Ochsenbauer C., Kappes J.C., Krueger W., Worden A., Schneider D., Zhu Z., Orentas R., Dimitrov D.S., Goldstein H, & Dropulić B. (2019). Multi-specific anti-HIV duoCAR-T cells display broad antiviral activity and potent elimination of HIV-infected cells in vivo. Science translational medicine, 11(504), eaav5685.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!