The scanning electron microscope (SEM) and transmission electron microscopy (TEM) images were obtained with the NTC JSM-6390LV SEM system and Hitachi H-7650 TEM system, respectively. The X-ray diffraction (XRD) analysis was conducted on the Rigaku Miniflex 600 system. The solution in 96-well ELISA plates for the absorbance determination was performed with the SpectraMax i3 multifunctional system.
H 7650 tem system
Manufactured by Hitachi
Sourced in Japan
The H-7650 TEM system is a transmission electron microscope (TEM) manufactured by Hitachi. It is designed to provide high-resolution imaging and analysis of samples at the nanoscale level. The H-7650 TEM system allows for the investigation of the structure, composition, and properties of materials at the atomic scale.
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5 protocols using h 7650 tem system
1
Characterization of Nanomaterials using Advanced Microscopy and Spectroscopy
2
Ultrastructural Analysis of Autophagy in Vascular Cells
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TEM (H-7650 TEM system, Hitachi Limited, Japan) was used to detect autophagosomes and autophagic lysosomes in the endarterial samples of female humans and mice, and in HUVECs as described in a previous study [30] (link). Briefly, fresh samples of human artery, mouse artery, and HUVECs were fixed overnight at 4 °C with 2.5% glutaraldehyde (g105905, Aladdin, Shanghai, China)-phosphate buffer (pH 7.2). After being fixed with 1% osmium tetroxide for 4 h, and then washed with PBS, the samples were dehydrated with gradient series of ethanol solutions. Then, the samples were embedded in epoxy resin and sliced with an ultra-slicer. Then, the samples were sprayed with gold to make them electrically conductive, placed on the sample platform, and imaged using a transmission electron microscope.
3
Tau Protein Visualization by TEM
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TEM was used for the morphological examination of tau. Briefly, tau samples (10 μL) were spotted onto a 200-mesh formvar-coated copper grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 5 min. The grid was then stained with 10 μL of 2% uranyl acetate for 30 s, and blotted with deionized water and air-dried at room temperature. The samples were detected using a Hitachi H7650 TEM system (Hitachi, Japan) at 80 kV with a × 60,000 magnification.
4
Ultrastructure Analysis of Turtle Pancreas
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Blocks of turtle pancreas approximately 1 mm3 in size were fixed in 2.5% glutaraldehyde in 0.1 MBPS solution (pH 7.4) at 4 °C overnight. Post-fixation of the turtle pancreas was done in 1% osmium tetroxide (Polysciences Inc. Warrington, PA, USA) for 1 h. The specimen washing was done in distilled water and stained en bloc with 2% aqueous uranyl acetate, then washed again in distilled water (3 × 5 min). Dehydration was done in ethanol, and specimens were embedded in Epon 812 R (Merck, Whitehouse Station, NJ). Ultrathin 50 nm-thick sections were mounted on copper grids and contrasted with uranyl acetate and lead acetate. Specimens were observed under the Hitachi TEM system (H-7650, Tokyo, Japan).
5
Electron Microscopy Sample Preparation
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Skin tissue sectioned to 1 mm3 in size was fixed overnight in 2.5% glutaraldehyde at 4°C. After PBS (0.1 M, pH 7.4) rinsing, 1% osmium tetroxide (Polysciences Inc. Warrington, Pennsylvania, USA) was used to fix the tissue at room temperature. Following the dehydration treatment, the tissue was embedded in Epon812 (Merck & Co Inc., New Jersey, USA) for 3 days at 60°C. The treated tissue samples were fine-sliced into 50 nm ultrathin sections and anchored on copper grids. Sections were stained with uranyl acetate and lead citrate, observed under the Hitachi TEM system (H-7650, Tokyo, Japan).
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