C myc a14

Manufactured by Santa Cruz Biotechnology

Sourced in United States

C-Myc-A14 is an antibody product offered by Santa Cruz Biotechnology. This antibody is designed for the detection of c-Myc protein in various biological samples. The core function of this product is to serve as a tool for researchers to identify and study the c-Myc protein, which is a transcription factor involved in cellular processes such as cell growth, proliferation, and differentiation.

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Lab products found in correlation

β tubulin, by Merck Group (1 mentions) Mouse monoclonal anti α tubulin clone b 5 1 2, by Merck Group (1 mentions) Anti gst z 5, by Santa Cruz Biotechnology (1 mentions) Irdye 800 conjugated anti mouse antibody, by Rockland Immunochemicals (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Mouse monoclonal anti ha, by Fortrea (1 mentions) Vinculin clone hvin 1, by Merck Group (1 mentions) Brefeldin a, by Thermo Fisher Scientific (1 mentions) 2 aminoethoxydiphenylborane 2 apb, by Merck Group (1 mentions) Gfp fl, by Santa Cruz Biotechnology (1 mentions) Ab1220, by Merck Group (1 mentions) Ab290, by Abcam (1 mentions) A2220, by Santa Cruz Biotechnology (1 mentions)

4 protocols using c myc a14

Western Blot Analysis of c-Myc and β-Tubulin

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Cell lines were cultured at a density of 600,000 cells per 60-mm plate, shifted into medium containing 10% fetal bovine serum for 24 h and 72 h. Twenty micrograms (20 μg) of whole-cell extracts were subjected to Western blot, performed as previously described 30 (link). The primary antibodies employed were the c-Myc-A14 (Santa Cruz, USA) and β-tubulin (Sigma, Spain) antibodies.

Vera O., Jimenez J., Pernia O., Rodriguez-Antolin C., Rodriguez C., Sanchez Cabo F., Soto J., Rosas R., Lopez-Magallon S., Esteban Rodriguez I., Dopazo A., Rojo F., Belda C., Alvarez R., Valentin J., Benitez J., Perona R., De Castro J, & Ibanez de Caceres I. (2017). DNA Methylation of miR-7 is a Mechanism Involved in Platinum Response through MAFG Overexpression in Cancer Cells. Theranostics, 7(17), 4118-4134.

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Western Blot Antibody Validation

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The primary antibodies used were mouse monoclonal anti-HA (Covance; RRID:AB_2314672), rabbit polyclonal purified antibody c-Myc (A-14) (Santa Cruz Biotechnology, Inc.; RRID:AB_631274) rat monoclonal anti-α-tubulin [YL1/2] (Novus Biologicals; RRID:AB_305328), mouse monoclonal anti-α-tubulin clone B-5-1-2 (Sigma-Aldrich; AB_477579) and rabbit polyclonal anti-GST (Z-5) (Santa Cruz; AB_631586). The secondary antibodies used were IRDye 800 conjugated anti-mouse antibody (Rockland Immunochemicals, Inc; RRID: RRID:AB_10703265), IRDye 800 conjugated anti-rabbit antibody (Rockland Immunochemicals, Inc; RRID:AB_220152), and IRDye700 conjugated anti-rat antibody (Rockland Immunochemicals Inc.; RRID: AB_220171). The blots were analyzed using the Odyssey Infrared Imaging system (LI-COR Biosciences).

Nuñez I., Rodriguez Pino M., Wiley D.J., Das M.E., Chen C., Goshima T., Kume K., Hirata D., Toda T, & Verde F. (2016). Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5. eLife, 5, e14216.

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Western Blot Antibody Sourcing

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Rabbit polyclonal antibodies against c-myc (A-14) and GFP (FL) were purchased from Santa Cruz Biotechnology. Rabbit polyclonal antibodies against GAPDH were purchased from Proteintech. Mouse monoclonal antibodies against vinculin, clone hVIN-1, were purchased from Sigma Aldrich (St. Louis, MO, USA). Mouse monoclonal antibodies against enterovirus VP1 (NCL-entero) were purchased from Novocastra (Buffalo Grove, IL, USA). XtremeGene HP plasmid transfection reagent was purchased from Sigma Aldrich. Brefeldin A (Invitrogen, Carlsbad, CA, USA) was dissolved in DMSO and used at a final concentration of 5 µg/mL. Also, 2-aminoethoxydiphenylborane (2APB, Sigma Aldrich) was diluted in DMSO and used at a final concentration of 100 µM.

Lennemann N.J., Evans A.S, & Coyne C.B. (2020). Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells. Viruses, 12(10), 1074.

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ChIP and Microarray Analysis of Chromatin

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ChIP and microarray analyses of immunoprecipitated chromatin were performed as described previously (Cam et al., 2005 (link)). Paraformaldehyde cross-linked chromatin was sheared to 500–1000 bp and immunoprecipitated with 1–3 μg of antibodies raised against green fluorescent protein (Abcam Ab290), lysine-9 dimethylated histone H3 (Abcam, Ab1220 or Ab115159), FLAG (Sigma-Aldrich, A2220), MYC (Santa Cruz Biotechnology, c-Myc A14) or recombinant Taz1 protein (kindly provided by Julia Cooper). Immunoprecipitated and whole cell extract DNA (WCE) were analyzed by multiplex PCR. The ratio of PCR product intensities of target to control (leu1) locus obtained from immunoprecipitated samples was normalized to the ratio of PCR products obtained from whole cell extract DNA. The oligonucleotides used for multiplex PCR are listed in Table S4. For microarray analyses, immunoprecipitated and whole cell extract DNA were amplified by random priming, labeled with Cy5 (IP DNA) or Cy3 (WCE), and hybridized to a custom designed Agilent 4×44K S. pombe 60mer array described previously (Cam et al., 2005 (link)). Enrichments were calculated as the ratio of normalized Cy5 (ChIP) to Cy3 (WCE) signal. For additional information see the Supplementary Experimental Procedures.

Zofall M., Smith D.R., Mizuguchi T., Dhakshnamoorthy J, & Grewal S.I. (2016). Taz1-Shelterin promotes facultative heterochromatin assembly at chromosome-internal sites containing late replication origins. Molecular cell, 62(6), 862-874.

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