43 he filter cube

Manufactured by Zeiss

The 43 HE filter cube is a component designed for optical microscopy applications. It is a modular optical filter that allows the selective transmission of specific wavelengths of light to facilitate specific imaging techniques. The core function of the 43 HE filter cube is to enable the isolation and detection of fluorescent signals within a sample.

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Lab products found in correlation

Axio observer z1, by Zeiss (9 mentions) Mrm monochrome camera, by Zeiss (3 mentions) 38 he filter cube, by Zeiss (3 mentions) Zen blue, by Zeiss (3 mentions)

3 protocols using 43 he filter cube

Epifluorescence Microscopy for GFP/mOrange2 Imaging

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For image acquisition, an epifluorescence microscope (AxioObserver Z1) from Zeiss (Jena, Germany) equipped with an X-Cite fluorescence light source and an MRm monochrome camera (Zeiss) was used. GFP fluorescence was recorded using a 38 HE filter cube (Carl Zeiss AG, Jena, Germany). mOrange2 fluorescence was recorded using the 43 HE filter cube (Carl Zeiss AG). The microscope manufacturer’s software (ZenBlue, Zeiss, Germany) controlled image acquisition. All images were captured using a 40x/0.75 NA EC PLAN NEOFLUAR lens.

Erickson J.L., Prautsch J., Reynvoet F., Niemeyer F., Hause G., Johnston I.G, & Schattat M.H. (2023). Stromule Geometry Allows Optimal Spatial Regulation of Organelle Interactions in the Quasi-2D Cytoplasm. Plant and Cell Physiology, 65(4), 618-630.

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Imaging GFP and mORANGE2 with Zeiss

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For image acquisition, an epi-fluorescence microscope (AxioObserver Z1) setup from Zeiss (Jena, Germany) equipped with an X-Cite fluorescence light source and an MRm monochrome camera (Zeiss, Jena, Germany) was used. GFP fluorescence was recorded using a 38 HE filter cube (Carl Zeiss AG, Jena, Germany). mORANGE2 fluorescence was recorded utilizing the 43 HE filter cube (Carl Zeiss AG. Jena, Germany). The microscope manufacturer’s software (ZenBlue, Zeiss, Germany) controlled image acquisition. All images were captured using a 40x/0.75 NA EC PLAN NEOFLUAR lens.

Prautsch J., Erickson J.L., Özyürek S., Gormanns R., Franke L., Lu Y., Marx J., Niemeyer F., Parker J.E., Stuttmann J, & Schattat M.H. (2022). Effector XopQ-induced stromule formation in Nicotiana benthamiana depends on ETI signaling components ADR1 and NRG1. Plant Physiology, 191(1), 161-176.

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Fluorescence Microscopy Imaging Protocol

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For image acquisition, an epi-fluorescence microscope (AxioObserver Z1) setup from Zeiss (Jena, Germany) equipped with an X-Cite fluorescence light source and a MRm monochrome camera (Zeiss, Jena, Germany) was used. GFP fluorescence was recorded using a 38 HE filter cube (Carl Zeiss AG, Jena, Germany). mORANGE2 fluorescence was recorded utilizing the 43 HE filter cube (Carl Zeiss AG. Jena, Germany). The microscope manufacturer's software (ZenBlue, Zeiss, Germany) controlled image acquisition. All images were captured using a 40x / 0.75 NA EC PLAN NEOFLUAR lens.

Prautsch J., Erickson J.L., Özyürek S., Gormanns R., Franke L., Parker J.E., Stuttmann J., & Schattat M. (2021). XopQ induced stromule formation inNicotiana benthamianais causally linked to ETI signaling and depends on ADR1 and NRG1.

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