Cd90.2 clone 53 2 1

Manufactured by BD

Sourced in Belgium

CD90.2 (clone 53-2.1) is a mouse monoclonal antibody that recognizes the CD90.2 antigen. CD90.2 is a widely expressed cell surface glycoprotein that is involved in various cellular processes. The antibody can be used for the identification and study of cells expressing CD90.2.

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Lab products found in correlation

Fixable viability dye fluortm 780, by Thermo Fisher Scientific (3 mentions) Diva software, by BD (3 mentions) Cd16 cd32 clone 2.4g2, by BD (2 mentions) Lsrfortessa flow cytometer, by BD (2 mentions) Rorγt clone b2d, by Thermo Fisher Scientific (2 mentions) Cd94 clone 20d5, by BioLegend (2 mentions) Nk1.1 clone pk136, by BD (1 mentions) Cd117 clone 2b8, by BD (1 mentions) Ter 119 clone ter119, by BD (1 mentions) Cd11b clone m1 70, by BD (1 mentions) Lsrfortessa sorp flow cytometer, by BD (1 mentions) Cd16 cd32 fc block clone 2.4g2, by BD (1 mentions) Klrg1 clone 2f1, by BD (1 mentions)

3 protocols using cd90.2 clone 53 2 1

Multiparametric Immune Cell Profiling

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Two million cells were stained with Fixable Viability Dye FluorTM 780 (eBioscience, Invitrogen) to perform a live/dead staining and preïncubated with CD16/CD32 (clone 2.4G2; BD Biosciences) to block non-antigen-specific binding of Igs. Combinations of anti-mouse fluorochrome-conjugated mAbs against CD45 (clone 30-F11), CD127 (clone SB/199), CD117 (clone 2B8), CD90.2 (clone 53-2.1), KLGR-1 (clone 2F1), NK1.1 (clone PK136), CD11b (clone M1/70), CD19 (clone 1D3), CD3e (clone G4.18), CD45RB (clone 16A), CD5 (clone 53-7.3), CD94 (clone 20d5), Gr-1 (clone RB6-8C5), TCRγδ (clone GL3) and Ter-119 (clone TER-119) (all from BD Biosciences) were used to label the cells (see gating strategy in Supplementary Figs. S1 and S2). Data acquisition was performed on a LSR Fortessa flow cytometer running DIVA software (BD Biosciences). Data analysis was performed by using FlowJo software.

Pollaris L., Decaesteker T., Van den Broucke S., Jonckheere A.C., Cremer J., Verbeken E., Maes T., Devos F.C., Vande Velde G., Nemery B., Hoet P.H, & Vanoirbeek J.A. (2020). Involvement of Innate Lymphoid Cells and Dendritic Cells in a Mouse Model of Chemical-induced Asthma. Allergy, Asthma & Immunology Research, 13(2), 295-311.

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Multicolor Flow Cytometry for Cellular Phenotyping

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Four million cells were stained with Fixable Viability Dye FluorTM 780 (eBioscience, Invitrogen, Merelbeke, Belgium) to perform a viability staining and pre-incubated with CD16/CD32 (clone 2.4G2, BD Biosciences, Erembodegem, Belgium) to block non-antigen-specific binding of immunoglobulins. Combinations of anti-mouse fluorochrome-conjugated mAbs against CD45 (clone 30-F11), CD127 (clone SB/199), CD90.2 (clone 53-2.1), KLRG-1 (clone 2F1), CD11b (clone M1/70), CD19 (clone 1D3), CD3e (clone G4.18), CD45RB (clone 16A), CD49b (Clone AK-7), CD5 (clone 53-7.3), TCRγδ (clone GL3), Ter-119 (clone TER-119) (all from BD Biosciences, Erembodegem, Belgium), RORγT (clone B2D)(ThermoFisher Scientific, Massachusetts, US), NKp46 (clone 29A1.4), Ly6G (Clone 1A8) and CD94 (clone 20d5)(all from Biolegend, San Diego, California) were used to label the cells. Sample acquisition was performed on a LSR Fortessa SORP flow cytometer running DIVA software (BD Biosciences, Erembodegem, Belgium). Data analysis was performed by using FlowJo software. Gating strategy available in Appendix A Fig. S4. Flow cytometry configuration are available in Appendix B.

Decaesteker T., Vanhoffelen E., Trekels K., Jonckheere A.C., Cremer J., Vanstapel A., Dilissen E., Bullens D., Dupont L.J, & Vanoirbeek J.A. (2021). Differential effects of intense exercise and pollution on the airways in a murine model. Particle and Fibre Toxicology, 18, 12.

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Multiparametric Flow Cytometry Protocol

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Four million cells were stained with Fixable Viability Dye Fluor TM 780 (eBioscience, Invitrogen, Merelbeke, Belgium) to perform a live/dead staining and preincubated with CD16/CD32 (FC block, clone 2.4G2, BD Biosciences, Erembodegem, Belgium). Combinations of anti-mouse fluorochrome-conjugated mAbs against CD45 (clone 30-F11), CD127 (clone SB/199), CD90.2 (clone 53-2.1), KLRG-1 (clone 2F1), CD11b (clone M1/70), CD19 (clone 1D3), CD3e (clone G4.18), CD45RB (clone 16A), CD49b (Clone AK-7), CD5 (clone 53-7.3), TCRγδ (clone GL3), Ter-119 (clone TER-119) (all from BD Biosciences, Erembodegem, Belgium), RORγT (clone B2D) (ThermoFisher Scientific, Massachusetts, US), NKp46 (clone 29A1.4), Ly6G (Clone 1A8) and CD94 (clone 20d5) (all from Biolegend, San Diego, California) were used to label the cells (gating strategy see Fig. S6). Data acquisition was performed on a LSR Fortessa flow cytometer running DIVA software (BD Biosciences, Erembodegem, Belgium). Data analysis was performed by using FlowJo software.

Decaesteker T., Jonckheere A.C., Vanhoffelen E., Schauvaerts J., Verhalle T., Cremer J., Dilissen E., Rodewald H.R., Dupont L., Bullens D.M.A, & Vanoirbeek J.A.J. (2022). Chlorine exposure and intensive exercise induces airway hyperreactivity in a 3-week murine exercise model. The Science of the total environment, 843.

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