Hank s balanced salt solution hbss

The MMO (QLNWD) [13 (link)] used for the experiments was synthesized by China Peptides Co., Ltd. (Shanghai, China). The CTX and levamisole were provided by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). An H&E staining kit was supplied by Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). Sheep red blood cells (SRBC) and guinea pig serum were obtained from Zhengzhou Baiji Biological Engineering Co. Ltd. (Henan, China). A mouse IgG enzyme linked immunosorbent assay (ELISA) kit was purchased from Shanghai Fusheng Industrial Co. Ltd. (Shanghai, China). Hanks’ balanced salt solution (HBSS) and ConA were purchased from Solarbio (Beijing, China). NLRP3 rabbit monoclonal antibody was purchased from Cell Signaling Technology (Massachusetts, USA). A 3,3′-diaminobenzidine (DAB) immunohistochemistry color development kit was purchased from BBI Life Science Corporation Co., Ltd. (Shanghai, China). Ammonium-chloride-potassium (ACK) lysis buffer was offered by Beyotime Biotechnology (Shanghai, China).

Dulbecco’s modified Eagle medium/F12 + Glutamax™-1, Neurobasal™-A Medium, Glutamax™, fetal bovine serum, B27 supplements, antibiotics (penicillin and streptomycin), 0.25% trypsin–EDTA, and FluoZin3-AM were purchased from Gibco (Grand Island, NY, USA). Hank’s balanced salt solution (HBSS) was purchased from Solarbio (Beijing, China). DNase, cytosine β-d-arabinofuranoside (Ara-C), TPEN, poly-l-lysine, TEA-Cl, 4-AP, and tetrodotoxin were purchased from Sigma-Aldrich (MO, USA). 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was obtained from Amresco, Inc. (Solon, OH, USA). The chemical constructs of Aβ peptides were synthesized by China Peptides Co., Ltd. (Shanghai, China) using the Aβ_25–35 sequence of human APP. Aβ_25–35 was dissolved in ddH₂O to prepare a stock solution with a concentration of 100 mM. The concentration of Aβ_25–35 used in the experiments in this study was 20 μM. Neonatal Sprague–Dawley rats were purchased from SPF Biotechnology Co., Ltd. (Beijing, China). All experimental protocols were approved by the Ethics Committee of Nankai University.

Glimepiride (purity 99.8%), gliclazide (purity 99.9%), and gliquidone (purity 99.3%) were supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Fetal bovine serum (FBS) and Hank’s Balanced Salt Solution (HBSS) was from Solarbio Co., Ltd (Beijing, China). Dulbecco’s modified Eagle’s Medium (DMEM) was from Solarbio Co., Ltd. Methanol and ethyl acetate were from Merck Co. Ltd (Darmstadt, Germany). NADPH, 6-phosphate- glucose, and 6-p-glucose dehydrogenase were purchased from Solarbio Co., Ltd. Human CYP2C9*1, *2 and *3 recombinases (1 nmol/ml P450, 10 mg/ml protein) were provided by the Research Institute for Liver Diseases (Shanghai Corporation Limited, Shanghai, China). All other chemicals and solvents were of the highest grade or analytical grade commercially available.