Human DU-145 CRPC cells (ATCC) were cultured in RPMI1640 medium (Corning Life Sciences, Corning, NY, USA) containing 10% heat-inactivated FBS. Human NCI-H660 NEPC cells (ATCC) were cultured in RPMI1640 medium containing 5% heat-inactivated FBS, 10 nM β-estradiol (Millipore Sigma), 10 nM hydrocortisone, 1% insulin-transferrin-selenium (ThermoFisher Scientific, Waltham, MA, USA) and 2 mM L-glutamine (ThermoFisher Scientific). Human MDA-MB-436 TNBC cells (ATCC) were cultured in Leibovitz’s L-15 medium (ThermoFisher Scientific) containing 10% heat-inactivated FBS. Human MDA-MB-468 TNBC cells (ATCC) were cultured in Leibovitz’s L-15 medium (ThermoFisher Scientific) containing 10% FBS. Cells were treated with the MUC1-C inhibitor GO-203 [17 (link)]. Cells were maintained in culture for 3–4 months. Authentication of the cells was performed by short tandem repeat (STR) analysis. Cells were monitored for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA).
Human nci h660 nepc cells
Manufactured by American Type Culture Collection
Sourced in United States
The Human NCI-H660 NEPC cells are a well-characterized cell line derived from a patient with neuroendocrine prostate cancer (NEPC). These cells can be used for research purposes to study the biology and characteristics of NEPC.
Automatically generated - may contain errors
Lab products found in correlation
Insulin transferrin selenium, by Thermo Fisher Scientific (4 mentions)
L glutamine, by Thermo Fisher Scientific (4 mentions)
β estradiol, by Merck Group (4 mentions)
Mycoalert mycoplasma detection kit, by Lonza (4 mentions)
Hydrocortisone, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Corning (3 mentions)
Charcoal stripped fbs, by Merck Group (3 mentions)
Heat inactivated fbs, by Gemini Bio (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Human du 145 crpc cells, by American Type Culture Collection (1 mentions)
Human mda mb 468 tnbc cells, by American Type Culture Collection (1 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
Human recombinant ifn γ, by STEMCELL (1 mentions)
4 protocols using human nci h660 nepc cells
1
Culturing and Characterizing Cancer Cell Lines
2
Cell Culture Conditions for Prostate Cancer Lines
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Human DU-145 (ATCC) cells were cultured in RPMI1640 medium (Corning Life Sciences, Corning, NY, USA) containing 10% heat-inactivated FBS (GEMINI Bio-Products, West Sacramento, CA, USA). Human LNCaP-AI cells were grown in phenol red-free RPMI1640 medium (ThermoFisher Scientific, Waltham, MA, USA) containing 10% charcoal-stripped FBS (Millipore Sigma, Burlington, MA, USA) [12 (link)]. Human NCI-H660 NEPC cells (ATCC) were cultured in RPMI1640 medium with 5% FBS, 10 nM β-estradiol (Millipore Sigma), 10 nM hydrocortisone, 1% insulin-transferrin-selenium (Thermo Fisher Scientific) and 2 mM L-glutamine (Thermo Fisher Scientific). Cells were treated with the MUC1-C inhibitor GO-203 [12 (link)]. Authentication of the cells was performed by short tandem repeat (STR) analysis. Cells were monitored for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA). Studies were performed on cells cultured for 3–4 months.
3
Prostate Cancer Cell Line Culture and Treatment
4
Cell Culture Conditions for Prostate Cancer Models
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Human DU-145 cells (ATCC) were cultured in RPMI1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (FBS; GEMINI Bio-Products, West Sacramento, CA, USA), 100 μg/ml streptomycin and 100 U/ml penicillin. LNCaP-AI cells were grown in phenol red-free RPMI1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% charcoal-stripped FBS (Millipore Sigma, Burlington, MA, USA).19 (link) Human NCI-H660 NEPC cells (ATCC) were cultured in RPMI1640 medium with 5% FBS, 10 nM β-estradiol (Millipore Sigma), 10 nM hydrocortisone, 1% insulin-transferrin-selenium (Thermo Fisher Scientific) and 2 mM L-glutamine (Thermo Fisher Scientific). Cells were treated with 10 ng/ml human recombinant IFN-γ (Stemcell Technologies, Vancouver, BC, Canada). Authentication of the cells was performed by short tandem repeat (STR) analysis every 4 months. Cells were monitored for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA) every 3 months.
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