Thermo tsq quantum access max

Sample extraction methodology was performed as described in Peterson et al. (2021) (link). Briefly, TSP filters were weighed individually, and two filters per sampled location were placed in a 50-mL centrifuge tube to ensure sufficient sample mass for analysis. Filters were spiked with internal standard (TCPP), and 45 mL methylene chloride:acetone (1:1) was added, followed by heated sonication (40 °C) for 1 hr. acetonitrile:water (1:1) was added to the centrifuge tube. Tubes were capped and placed on an orbital shaker platform (Model MaxQ 4000, Thermo Scientific, Waltham, MA) for 18 hrs at 350 rpm. Next, tubes were centrifuged (10 min; 3100 rpm) and supernatant was decanted into a second 50-mL centrifuge tube. Magnesium sulfate (4 g) and sodium chloride (1 g) salts were added, and tubes were vortexed for 1 min followed by centrifugation for 10 min at 3100 rpm. The final supernatant was then combined with the initial supernatant in the glass vial, evaporated under a stream of nitrogen at 35 °C, and reconstituted in 1 mL acetonitrile. Extract was vortexed for 10 s and syringe filtered (PTFE; 0.22 μm) into amber chromatography vials for subsequent analysis via triple quadrupole liquid chromatography tandem mass spectrometry with electrospray ionization (Thermo TSQ Quantum Access Max, Thermo Scientific, Waltham, MA), as described in Peterson et al. (2020) (link).