The concentration of rat ICAM/CD54, rat IL-1β, rat IL-18, rat IL-6, and rat TNF-α in plasma was determined using xMAP technology with the Luminex Performance Assay rat cytokine premixed kit (R&D Systems Inc., #FCST06). Standard and samples were reconstituted and diluted as described in the manual of the manufacturer. 5 × 104 cells were measured in a cytomics FC500 flow cytometer.
Luminex performance assay rat cytokine premixed kit
Manufactured by R&D Systems
The Luminex Performance Assay rat cytokine premixed kit is a multiplex assay designed to measure the concentration of multiple rat cytokines simultaneously in a single sample. The kit utilizes Luminex technology to quantify the target analytes.
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Lab products found in correlation
2 protocols using luminex performance assay rat cytokine premixed kit
1
Multiplex Cytokine Profiling in Rat Plasma
2
Kidney Injury Biomarkers and Immune Response
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Blood samples were taken before surgery and 6, 24 h, and in group 3–5 96 h after induction of AKI. Renal function was assessed in all animals by serum (s)-creatinine and serum-urea measurements using the dry-slide technology (Fuji-Dry Chem 4000 Autoanalyzer). The concentration of rat intercellular adhesion molecule (ICAM)/CD54, rat interleukin-1 beta (IL-1β), rat IL-18, rat IL-6, and rat tumor necrosis factor-alpha (TNF-α) in plasma was determined by a Multiplex FACS analysis using xMAP technology with the Luminex Performance Assay rat cytokine premixed kit (R&D Systems Inc., #FCST06). The leukocyte, macrophage, and T-cell infiltration rates were analyzed in kidney tissue samples using a cytomics FC500 flow cytometer.
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