Poly d lysine coated chamber slides

Manufactured by BD

Poly-D-Lysine-coated chamber slides are laboratory equipment designed to facilitate cell culture and microscopic analysis. The slides are coated with the positively charged amino acid polymer, poly-D-lysine, which promotes cell adhesion and growth. These slides are commonly used in various cell biology applications.

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Lab products found in correlation

Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Normal goat serum (ngs), by Merck Group (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Triton x 100, by Merck Group (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Merck Group (1 mentions)

Close spelling variants of this product

Poly-D-lysine/laminin–coated 8-well chamber slides Poly-D-lysine-coated 8 chamber polystyrene vessel tissue culture treated glass slide Poly-lysine coated chamber slides Poly-D-lysine-coated Poly-D-lysine Chamber slides

2 protocols using poly d lysine coated chamber slides

Mitochondrial Staining with MitoTracker

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Cells in 8-well poly-D-Lysine-coated chamber slides (BD Biosciences) were incubated in 200 nM MitoTracker^® Red CMXRos (Thermo Fisher Scientific; #M7512) diluted in serum-free media for 30 min at 37°C. Cells were then immediately fixed in 4% PFA and treated as for indirect immunofluorescence.

Rivera-Serrano E.E., DeAngelis N, & Sherry B. (2017). Spontaneous Activation of a MAVS-dependent Antiviral Signaling Pathway Determines High Basal Interferon-β Expression in Cardiac Myocytes. Journal of molecular and cellular cardiology, 111, 102-113.

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Immunocytochemistry of Cultured Cells

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Cells in poly-D-Lysine-coated chamber slides (BD Biosciences) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in phosphate-buffered saline (PBS) and permeabilized with 0.25% Triton X-100 (Sigma, #X100). Slides were blocked with normal goat serum (Sigma; #G9023), incubated in DAPI (4′,6-diamidino-2-phenylindole; Sigma; #D8417), immunostained with the indicated primary and secondary antibodies, and preserved with ProLong Gold (Invitrogen).

Rivera-Serrano E.E, & Sherry B. (2016). NF-κB Activation is Cell Type-Specific in the Heart. Virology, 502, 133-143.

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