Q tof lc ms 6510 series classic g6510a

The supernatant of garlic homogenate were filtered through 0.45 µm glass filter and diluted further to make appropriate solution. 20 µL was injected in LC-MS. The analysis was carried out on an Agilent 1200 series HPLC instrument (Agilent Technologies, USA) equipped with a quaternary pump (G13311A, USA), a de-gasser (G1322A, USA), a diode-array detector (G1315D, USA), an auto sampler (G1329A, USA) and a column compartment (G1316A, USA). Mass spectrometric detection was carried out on a quadrupole time-of-flight (Q-TOF) mass spectrometer (Q-TOF LC/MS 6510 series classic G6510A, Agilent Technologies, USA) equipped with an ESI source. The data acquisition was under the control of Mass Hunter workstation software. The typical operating source conditions for MS scan in positive ion ESI mode were optimized as follows; the fragmentor voltage was set at, 80 V; the capillary at, 3000–3500 V; the skimmer at, 60 V; nitrogen was used as the drying (300°C; 9 L/min) and nebulizing (45 psi) gas. For full scan MS mode, the mass range was set at m/z 100–2000. All the spectra were recorded under identical experimental conditions and are average of 20–25 scans.