For immunofluorescence, adult flies within 5–12 days were dissected in PBS, then subject in 4% PFA and fixed on ice for 1–4 hours. The brains were washed with the Washing Buffer (3% NaCl, 1% Triton X-100 in PBS) for three times, with 10 min for each time. Samples were transferred to Penetration/Blocking Buffer (2% Triton-X100, 10% Normal Goat Serum in PBS) for 20 hours at 4 °C on the shaker. Samples were then transferred into solution with primary antibody diluted with Dilution Buffer (0.25% Triton-X100, 1% Normal Goat Serum in PBS) for 24 hours at 4 °C. After washing three times, samples were incubated with the secondary antibody for 14–20 hours at 4 °C on the shaker. At last, samples were washed three times and mounted with 50% glycerol. The primary and secondary antibodies were rabbit anti-mCherry (1:500; Abcam Cat#ab167453, RRID: AB_2571870) and AlexFlour647 goat anti-rabbit (1:500; AAT Bioquest, Cat#16710). An inverted Ti-E A1 confocal microscope (Nikon) was used for immunofluorescence imaging. The confocal microscope was equipped with a 20×/0.75 NA air objective. A 640nm laser and 663/738 nm emission filter were used for this experiment.
Inverted ti e a1 confocal microscope
Manufactured by Nikon
The Inverted Ti-E A1 confocal microscope is a laboratory instrument used for high-resolution imaging of biological samples. It is designed to capture detailed images of cellular structures and processes by using a focused laser beam to scan the sample and collect light from specific focal planes. The instrument features advanced optics and detection systems to provide clear and precise images for scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using inverted ti e a1 confocal microscope
1
Immunofluorescence Imaging of Fly Brains
2
Immunofluorescence Imaging of Fly Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunofluorescence, adult flies within 5–12 days were dissected in PBS, then subject in 4% PFA and fixed on ice for 1–4 hours. The brains were washed with the Washing Buffer (3% NaCl, 1% Triton X-100 in PBS) for three times, with 10 min for each time. Samples were transferred to Penetration/Blocking Buffer (2% Triton-X100, 10% Normal Goat Serum in PBS) for 20 hours at 4 °C on the shaker. Samples were then transferred into solution with primary antibody diluted with Dilution Buffer (0.25% Triton-X100, 1% Normal Goat Serum in PBS) for 24 hours at 4 °C. After washing three times, samples were incubated with the secondary antibody for 14–20 hours at 4 °C on the shaker. At last, samples were washed three times and mounted with 50% glycerol. The primary and secondary antibodies were rabbit anti-mCherry (1:500; Abcam Cat#ab167453, RRID: AB_2571870) and AlexFlour647 goat anti-rabbit (1:500; AAT Bioquest, Cat#16710). An inverted Ti-E A1 confocal microscope (Nikon) was used for immunofluorescence imaging. The confocal microscope was equipped with a 20×/0.75 NA air objective. A 640nm laser and 663/738 nm emission filter were used for this experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!