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Ac240ts r3

Manufactured by Olympus

The AC240TS-R3 is a laboratory centrifuge designed for various sample preparation tasks. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force of 4,240 xg. The centrifuge has a compact footprint and can accommodate a variety of rotor types.

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2 protocols using ac240ts r3

1

Characterization of Amyloid Fibers by AFM

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JPK Nanowizard 3 AFM mounted on Olympus IX73 optical microscope was user to measure the sample in air. AFM probes (AC240TS-R3 from Olympus) with a nominal resonance frequency of about 70 kHz were used in tapping mode. The samples were dissolved in Milli-Q water to a final concentration of 8 mM by vortexing. The solution was left at room temperature for 24 h; thereafter, 20 μL was pipetted on a freshly cleaved mica sheet and immediately rinsed 3 times with 1 mL of Milli-Q water. The solution was left to dry in a vacuum chamber overnight. The AFM measurement was performed at 50% RH. Following the flattening of the AFM height channel, more than 20 orthogonal and longitudinal profiles were extracted to extrapolate the characteristic lengths of the fibers.
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2

Atomic Force Microscopy Characterization of Evaporated Samples

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After droplet deposition, liquid evaporates rapidly as monitored via bright field optical microscopy. To avoid any residual solvent and facilitate atomic force microscopy (AFM) characterization, the glass slides were placed in a clean ambient environment overnight unless specified. The AFM (MFP-3D, Oxford Instrument, Santa Barbara, CA) has a deflection configuration. Silicon probes (AC 240TS-R3, Olympus America, Central Valley, PA) with a force constant of 1.7 N/m and resonance frequency of 70 kHz. Tapping mode was utilized for imaging with damping set at 40–60%. The AFM images were analyzed using the Asylum MFP-3D software developed on an Igor Pro 6.12 platform.
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