Actb flpe

Manufactured by Jackson ImmunoResearch

ACTB-FLPe is a recombinant protein that functions as a Flp recombinase enzyme. Flp recombinase is derived from the 2-micron plasmid of Saccharomyces cerevisiae and catalyzes site-specific recombination between Flp recognition target (FRT) sites.

Automatically generated - may contain errors

Lab products found in correlation

Villin cre, by Jackson ImmunoResearch (1 mentions) Eiia cre, by Jackson ImmunoResearch (1 mentions) Plzf cre, by Jackson ImmunoResearch (1 mentions) B6 albino, by Charles River Laboratories (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions) Gad2 cre, by Jackson ImmunoResearch (1 mentions) Tdtomato reporter, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Wnt1 cre2, by Jackson ImmunoResearch (1 mentions)

4 protocols using actb flpe

Generation of Il22 Reporter Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Il22^hCD4.fl reporter and floxed mice were generated by targeting an IRES and truncated hCD4 gene into the fifth exon (between the stop codon and 3’ untranslated region), and loxP sites that flanked the entire Il22 gene (see Method Details below). B6 Albino (CRL 022) and CD-1 (CRL 493) used for generating chimeric mice were purchased from Charles River Laboratories (CRL). C57BL/6 (WT; JAX 000664), Actb-Flpe (JAX 003800), EIIa-cre (JAX 003724), Plzf-cre (JAX 024529), mCd4-cre (JAX 022071), Rag1^–/– (JAX 002216), Sting1^fl/fl (JAX 031670) and Villin-cre (JAX 021504) mice were purchased from Jackson Laboratory (JAX). Rorc^EGFP mice were kindly provided by Dr. Gerard Eberl (Lochner et al., 2008 ) (Instit Pasteur, France). Aicda^–/–.µs^–/– mice were kindly provided by Dr. Frances E. Lund (UAB). S100a9^–/– mice were kindly provided by Dr. Thomas Vogl (Manitz et al., 2003 (link)) (Instit Immunology, Germany). In most experiments, littermates were used as controls and experimental adult animals (8–12 wk old) were co-caged in groups of 2–7 mice. Both sexes were used per experimental group whenever possible. All mouse strains were bred and maintained at UAB in accordance with IACUC guidelines.

Zindl C.L., Witte S.J., Laufer V.A., Gao M., Yue Z., Janowski K.M., Cai B., Frey B.F., Silberger D.J., Harbour S.N., Singer J.R., Turner H., Lund F.E., Vallance B.A., Rosenberg A.F., Schoeb T.R., Chen J.Y., Hatton R.D, & Weaver C.T. (2022). A Non-redundant Role for T cell-derived Interleukin 22 in Antibacterial Defense of Colonic Crypts. Immunity, 55(3), 494-511.e11.

+ Open protocol

+ Expand

Novel Mouse Lines for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

All procedures were performed according to the guidelines of the Institutional Animal Care and Use Committee (IACUC) at Rockefeller University. C57BL/6J (stock no. 000664), ACTB-FLPe (stock no. 005703), tdTomato reporter (stock no. 007914), GAD2-Cre (stock no. 019022) mice were obtained from the Jackson Lab. Nova1-KO mice were generated in Robert Darnell lab^{32 (link)} and back-crossed to C57BL/6J strain at least 10 times. Mice were housed in a 12-h light/dark cycle, up to 5 mice per cage. Male or female mice aged 8–12 weeks were used for animal experiments unless otherwise stated. Littermates of the same-sex were randomly assigned to experimental groups.

Tajima Y., Ito K., Yuan Y., Frank M.O., Saito Y, & Darnell R.B. (2023). NOVA1 acts on Impact to regulate hypothalamic function and translation in inhibitory neurons. Cell reports, 42(2), 112050.

+ Open protocol

+ Expand

Genetic Mouse Models for Epigenetic Regulation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wnt1-Cre2 (Jackson Laboratory, 022137)(Lewis et al., 2013 (link)), Mesp1-Cre (Saga et al., 1999 (link)), R26R-LacZ (Soriano, 1999 (link)), and Hdac2^Flox (Anokye-Danso et al., 2011 (link)) mice have been previously described. Frozen embryos of Hdac1^{tm1a(EUCOMM)Wtsi} (strain EM:04097) were obtained from the European Mouse Mutant Archive (EMMA). The University of Massachusetts Medical School Transgenic Animal Facility regenerated cryopreserved embryos. The knockout-first allele, Hdac1^{tm1a(EUCOMM)Wtsi}, mice were bred with wild type mice and are annotated Hdac1^LacZ/+. Hdac1^{tm1a(EUCOMM)Wtsi} (Hdac1^LacZ) mice were bed with ACTB-FLPe (Jackson Laboratory, 003800) to generate Hdac1^Flox mice. Hdac1^Flox mice were bred with Hdac2^Flox, R26R-LacZ, and Wnt1-Cre2 mice to generate mice for analysis. All animal protocols were approved by the University of Massachusetts Medical School Institutional Animal Care and Use Committee (IACUC).

Milstone Z.J., Lawson G, & Trivedi C.M. (2017). Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development and craniofacial morphogenesis. Developmental dynamics : an official publication of the American Association of Anatomists, 246(12), 1015-1026.

+ Open protocol

+ Expand

Generation of Hmgb1 Conditional Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

A loxP site (L83) and a FRT-Neo-FRT-LoxP (FNFL) cassette were engineered by recombineering to flank exons 2–4 of the Hmgb1 gene, thus generating the Hmgb1 conditional targeting construct on a bacterial artificial chromosome. The FNFL cassette conferred G418 resistance during gene targeting in PTL1 ES cells, and the DTA cassette provided autonomous negative selection to reduce random integration. After transfection of the targeting construct, five targeted ES cell clones were identified by PCR screening. Two clones were injected into C57BL/6 blastocysts to generate chimeric mice. Two chimeric males conferred germline transmission. Male chimeras were bred to mice expressing FLPe recombinase under the human ACTB promoter (ACTB:FLPe, obtained from Jackson labs) mice to remove the Neomycin cassette. Two founder lines, one from each ES clone, carrying the Hmgb1 conditional allele were selected to determine the effect of homozygosity and Cre-mediated deletion. As we observed no abnormalities in homozygous mice and efficient Hmgb1 deletion in both lines, we conducted all subsequent experiments in one of the two founder lines. For the current study, mice were backcrossed to C57Bl/6 mice for 5 generations.

Huebener P., Gwak G.Y., Pradere J.P., Quinzii C.M., Friedman R., Lin C.S., Trent C.M., Mederacke I., Zhao E., Dapito D.H., Lin Y., Goldberg I.J., Czaja M.J, & Schwabe R.F. (2014). High Mobility Group Box 1 is Dispensable for Autophagy, Mitochondrial Quality Control and Organ Function in Vivo. Cell metabolism, 19(3), 539-547.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!