Agarose

Colistin sulfate and sodium borohydride (NaBH₄) were purchased from Sigma Aldrich, Inc., St. Louis, MO, USA. Sodium dodecyl sulfate (SDS), sodium sulfate, sodium phosphate dibasic, and sodium dihydrogen orthophosphate were procured from LOBA Chemie PVT. LTD., Mumbai, India. Silver nitrate (AgNO₃), hydrochloric acid (HCl), potassium bromide (KBr), sodium chloride (NaCl), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), and acetonitrile (ACN) were analytical grade and procured from RCI Labscan Limited, Bangkok, Thailand. Mueller Hinton (MH) and Luria Bertani (LB) agar and broth were obtained from Titan Biotech Ltd., Delhi, India. Agarose and EDTA were purchased from Bio Basic Canada Inc., Markham, Ontario, Canada. Quick-Loaded^® DNA ladder was from New England BioLabs Inc., Herts, England. Safe-Green dye was purchased from Applied Biological Materials Inc., Richmond, BC, Canada. E.Z.N.A.^® bacterial DNA kit was obtained from Omega Bio-tek Inc., Norcross, GA, USA. E. coli TISTR 887, P. aeruginosa TISTR 357, and K. pneumonia TISTR 1383 were from the Thailand Institute of Scientific and Technology Research, Pathum Thani, Thailand.

Majority of bacteriological media used in this study were purchased from Oxoid Co., UK, unless otherwise stated. Proteinase K and Dream Taq Green Master Mix 2X were purchased from Thermo Scientific Co., USA. Isopropanol, acetone, acetonitrile, toluene, methyl alcohol, and ethyl alcohol were purchased from Sigma, Co., USA. Lysozyme and agarose were purchased from Bio Basic Inc., Canada. DNA ladder (100 bp DNA Ladder H3 RTU (Ready-to-Use) was purchased from GeneDirex, Inc., Taiwan. Universal primers specific for 16S rDNA gene were synthesized by Bioneer Co., Korea.

Agarose was purchased from Bio Basic Inc. (Markham, ON, Canada). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS), and penicillin/streptomycin (pen/strep) were purchased from Gibco (Grand Island, NY, USA). A 6-well plate was obtained from Corning (Corning, NY, USA). Poly (d, l-lactide-co-glycolide) (PLGA), Temozolomide (TMZ), Deferiprone (DFP), Span 80^®, and sodium alginate were purchased from Sigma (St. Louis, MO, USA). liquid paraffin was obtained from Caledon Laboratories (Georgetown, ON, Canada)).

Davis minimal medium (DMM), 4-aminoantipyrine, potassium ferrocyanide, catechol, sodium hydroxide pellets used in this study were purchased from HiMedia Laboratories Pvt. Ltd. Mumbai, phenol, 2-mercaptoethanol, ammonium hydroxide, glycerol, disodium EDTA, primers, acids and solvents were procured from Sigma, Aldrich, Tris Base, Agarose from Bio Basic Inc. All the following heavy metals CoCl₂.2H₂O, ZnSO₄.H₂O, (CH₃COO)₂Pb.3H₂O, HgCl₂, CdCl₂, CuSO₄.5H₂O, NiCl₂.6H₂O, MnCl₂.4H₂O, and K₂Cr₂O₇ were purchased from Merck Millipore, Taq DNA polymerase Master Mix from Ampliqon and PCR purification Kit from Favorgen Biotech Corp. were used.