Antibodies against phospho-mTOR (No. 5536), mTOR (No. 2983), phospho-p70S6K (No. 9208), p70S6K (No. 2708), β-catenin (No. 9562), OCT-4 (No. 2750), SOX-2 (No. 4900), NANOG (No. 4893), and SSEA-4 (No. 4755) were obtained from Cell Signaling Technology, Inc. (Danvers, MA). FGF2 (sc-74412) and β-actin (sc-69879) antibodies were from Santa Cruz Biotechnology (Dallas, TX). Human CXCR2 antibody was purchased from Abcam (ab21641; Cambridge, UK). The source of recombinant human growth-related oncogene α (GROα) was R&D Systems, Inc. (P09341; Minneapolis, MN). Alexa488 (No. A11034) and 4′,6-diamidino-2-phenylindole (DAPI) solutions (No. D1306) were from Invitrogen (Carlsbad, CA). The small molecule inhibitors SB225002 (No. 2725) and SB265610 (No. 2724) were purchased from Tocris Bioscience (Bristol, UK). PNU 74654 (P0052), FK506 (F4679), and rapamycin (R8781) were from Sigma-Aldrich Corporation (St. Louis, MO). The mTOR inhibitor RAD001 (everolimus) was purchased from InvivoGen (San Diego, CA). BC2059 (A14381) was obtained from AdooQ Bioscience LLC (Irwin, CA).
Fgf2 sc 74412
Manufactured by Santa Cruz Biotechnology
Sourced in United States
FGF2 (sc-74412) is a recombinant protein that represents the full-length human Fibroblast Growth Factor 2 (FGF2). FGF2 is a member of the fibroblast growth factor family and plays a role in a variety of cellular processes, including cell growth, differentiation, and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fgf2 sc 74412
1
Stem Cell Differentiation Pathway Analysis
2
Immunohistochemical Evaluation of Angiogenic Factors
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The immunohistochemistry staining was used to evaluate VEGFA and FGF-2 positive expressions in the tissues. The histopathology slides were processed immunohistochemically using horse radish-labeled monoclonal antibodies (anti-VEGFA #SC-7269 (
Santa Cruz BiotechnologyInc., California, USA), FGF-2 #SC-74412 (
Santa Cruz BiotechnologyInc., California, USA)), and 3–3′ diaminobenzidine (DAB) (Abcam, USA). Then, the positive expression of the protein marked by brown precipitate on the cells of the oral ulcer site was observed using an inverted light microscope with 100 × , 400 × , and 1000× magnification in five different fields of view by two observers.
Santa Cruz BiotechnologyInc., California, USA), FGF-2 #SC-74412 (
Santa Cruz BiotechnologyInc., California, USA)), and 3–3′ diaminobenzidine (DAB) (Abcam, USA). Then, the positive expression of the protein marked by brown precipitate on the cells of the oral ulcer site was observed using an inverted light microscope with 100 × , 400 × , and 1000× magnification in five different fields of view by two observers.
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