Dxh 900 hematology analyzer

Blood samples were collected and tested for creatinine, estimated glomerular filtration rate, complement component C3 (C3), complement component C4 (C4), c-reactive protein (CRP), and lactate dehydrogenase using serum. Samples for sC5b-9, factor H, factor I, and midkine were collected in 2 mL EDTA tubes and placed immediately on ice. These samples were separated by centrifugation at 3,000 rpm at 4°C and the plasma frozen at −70°C until the assay was performed. Plasma sC5b-9, factor I, and midkine were all measured using colourimetric ELISA (Microvue sC5b-9 Plus Enzyme Immunoassay Quidel Corporation; Hycult Biotech; Cellmid Ltd.) as per manufacturer’s instructions. Factor H was assayed via nephelometry using an in-house method adapted from Sofat et al. [8] (link) (Siemens BN2, Binding Site anti-fH). Haemoglobin was measured photometrically at 525 nm on the DxH 900 Hematology Analyzer (Beckman Coulter, Inc., Brea, CA, USA). The platelet count was derived from the Coulter principle, also on the DxH 900 Hematology Analyzer (Beckman Coulter, Inc., Brea, CA, USA). CD46 was analysed using whole blood via flow cytometry (Beckman Coulter Navios Flow Cytometer, Life Sciences, Indianapolis, USA).

Age, sex, weight, vital signs, and hourly urine output were all recorded since admission. Patients were classified according to their underlying medical history, site of infection, and vasopressors use. Glasgow, SOFA, and APACHE II scores were used to assess patient admission. We evaluated septic shock, mechanical ventilation, renal replacement therapy, length of ICU stay, and mortality within 28 days in all patients. Tests such as complete blood count, ALT, AST, total bilirubin, albumin, IL-6, procalcitonin, lactate, mineral panel, blood gas, baseline creatinine, and thrombomodulin were tested immediately upon admission. For the thrombomodulin test (Abcam brand), the blood sample was taken into a serum separator tube. After the formation of a clot, the blood sample was centrifuged at 2000 rpm for 10 minutes, then stored at −20 degrees Celsius or lower, avoiding repeating freezing and thawing for patient samples, which were tested by ELISA technique. We used Cobas^® 8000 modular analyzer series - Roche Diagnostic and DxH 900 hematology analyzer - Beckman Coulter. The tests were conducted at the Department of Biochemistry and Hematology and Blood Transfusion Center of Hue Central Hospital, Vietnam.