Sodium acetate

Manufactured by Avantor

Sourced in United States, Poland, Germany, United Kingdom, Belgium, Austria

Sodium acetate is a chemical compound with the formula CH3COONa. It is a white, crystalline solid that is commonly used in various laboratory applications. Sodium acetate serves as a buffer agent, stabilizing the pH of solutions.

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Lab products found in correlation

Acetic acid, by Avantor (11 mentions) Hydrochloric acid (hcl), by Avantor (7 mentions) Sodium carbonate, by Avantor (7 mentions) Sodium hydroxide (naoh), by Avantor (6 mentions) Methanol, by Avantor (5 mentions) Formic acid, by Merck Group (5 mentions) Potassium chloride (kcl), by Avantor (4 mentions) Ethanol, by Avantor (4 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Ethylene glycol, by Avantor (3 mentions) Methanol, by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (3 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (3 mentions) Chlorogenic acid, by Merck Group (3 mentions) Folin ciocalteu reagent, by Merck Group (3 mentions) Quercetin, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) Distilled water, by Merck Group (3 mentions) Ammonium hydroxide solution, by Avantor (3 mentions)

Spelling variants of this product

Sodium acetate trihydrate (19 citations) Sodium acetate anhydrous (6 citations) Sodium acetate trihydrate (NaOAc.3H2O) (2 citations)

48 protocols using sodium acetate

Photoresist-Based Biomaterial Functionalization

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OrmoPrime08, S1805 and SU-8 photoresist,
SU-8 2000 thinner, and an AZ303 developer were purchased from Micro
Resist Technology (Germany). Dimethyl sulfoxide (DMSO), acetic acid,
propylene glycol monomethyl ether acetate, and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDC) were obtained from Sigma Aldrich (Germany). pNIPAAm-based terpolymer
composed of N-isopropylacrylamide, methacrylic acid,
and 4-methacryloyloxybenzophenone (in a ratio of 94:5:1), benzophenone
disulfide, and 4-sulfotetrafluorophenol (TFPS) were synthesized in
our laboratory as previously reported.^{43 (link)−45 (link)}IgG from mouse
serum (mIgG, I 5381), Tween 20 (P9416), and bovine serum albumin (A2153)
were purchased from Sigma Aldrich (Austria). Phosphate-buffered saline
(PBS) and sodium acetate were obtained from VWR Chemicals (Austria).
Alexa Fluor 790 goat anti-mouse IgG (a-mIgG, A11375) was acquired
from Life Technologies (Eugene, OR).

Quilis N.G., Hageneder S., Fossati S., Auer S.K., Venugopalan P., Bozdogan A., Petri C., Moreno-Cencerrado A., Toca-Herrera J.L., Jonas U, & Dostalek J. (2020). UV-Laser Interference Lithography for Local Functionalization of Plasmonic Nanostructures with Responsive Hydrogel. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 124(5), 3297-3305.

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Haematococcus pluvialis Culture Protocol

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Haematococcus pluvialis (SAG number 192.80) was obtained from the Culture Collection of Algae at the University of Göttingen, Germany. Bold Modified Basal Medium (BBM) was used as culture medium. This was prepared by diluting 20 mL BBM freshwater nutrient solution (50 × concentrate) from Sigma-Aldrich (Germany) with 980 mL deionised water, obtaining the following composition (per litre): 11.42 mg H₃BO₃, 25.0 mg CaCl₂·2H₂O, 0.49 mg Co(NO₃)₂·6H₂O, 1.57 mg CuSO₄·5H₂O, 50.0 mg EDTA (free acid), 4.98 mg FeSO₄·7H₂O, 75 mg MgSO₄·7H₂O, 1.44 mg MnCl₂·4H₂O, 0.71 mg MoO₃, 0.003 mg NiCl₂·6H₂O, 31.0 mg KOH, 0.003 mg KI, 175.0 mg KH₂PO₄, 75 mg K₂HPO₄, 25 mg NaCl, 250.0 mg NaNO₃, 0.002 mg Na₂SeO₃, 0.001 mg SnCl₄, 0.0022 mg VOSO₄·3H₂O and 8.82 mg ZnSO₄·7H₂O. Additionally, 1.64 g of sodium acetate (molecular biology grade, >99.0%) from VWR Chemicals (Germany) was added to the medium. The pH of the culture medium was 6.8.

Bauer A, & Minceva M. (2019). Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatography. RSC Advances, 9(40), 22779-22789.

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Analytical Method for HCQ Purity

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The HCQ with certified purity of 99.2% was obtained from SCI pharma, Taiwan. AR grade ortho phosphoric acid, hydrochloric acid, potassium dihydrogen phosphate, 1-pentane sulfonic acid, NaOH, sodium acetate, and acetic acid chemicals were purchased from VWR chemicals, Radnor, PA, USA. HPLC-grade acetonitrile (99.9%) and methanol from J. T. Baker were procured from VWR chemicals, Radnor, PA, USA. High-quality HPLC-grade purified water was used throughout the experiments.

Dongala T., Katari N.K., Ettaboina S.K., Krishnan A., Tambuwala M.M, & Dua K. (2021). In vitro Dissolution Profile at Different Biological pH Conditions of Hydroxychloroquine Sulfate Tablets Is Available for the Treatment of COVID-19. Frontiers in Molecular Biosciences, 7, 613393.

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Targeted Multi-Residue Analysis of Pesticides

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UPLC grade methanol (MeOH), acetonitrile (ACN), ethyl acetate, and water were obtained from Actu-all chemicals (Oss, Netherlands). Formic acid (FA), acetic acid (HAc), sodium acetate, ammonium formate, and ammonium carbonate were obtained from VWR International (Darmstadt, Germany). Magnesium sulfate, dimethyl sulfoxide (DMSO), β-glucoronidase/arylsulfatase from Helix Pomatia, dipotassium phosphate, and potassium dihydrogen phosphate were obtained from Sigma-Aldrich (St. Louis, USA). Bondesil PSA was obtained from Agilent Technologies (Santa Clara, USA). Bakerbond C18 was purchased from Avantor Performance Materials (Phillipsburg, USA).
Analytical reference standards and isotope labelled internal standards were purchased from LGC Standards (Teddington, UK), Toronto Research Chemicals (Toronto, Canada), Sigma-Aldrich, HPC Standards (Cunnersdorf, Germany), Bayer CropScience (Monheim am Rhein, Germany), and MerchaChem (Nijmegen, Netherlands). Reference standards of chlorpyrifos-methyl-desmethyl and chlorpyrifos-desethyl were kindly donated by CVUA (Stuttgart, Germany).
Oasis HLB 60 µm/60 mg 96-well plates were purchased from Waters (Milford, USA), Strata-X polymeric reversed phase cartridges (200 mg/6 mL) were purchased from Phenomenex (Utrecht, the Netherlands), and Amicon Ultra 30 kDa centrifugal filter units were purchased from Sigma-Aldrich.

Nijssen R., Lommen A., van den Top H., van Dam R., Meuleman-Bot C., Tienstra M., Zomer P., Sunarto S., van Tricht F., Blokland M, & Mol H. (2023). Assessment of exposure to pesticides: residues in 24 h duplicate diets versus their metabolites in 24 h urine using suspect screening and target analysis. Analytical and Bioanalytical Chemistry, 416(3), 635-650.

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Ferric Reducing Antioxidant Power Assay

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The FRAP reaction mixture was prepared mixing 300 mM sodium acetate (VWR International, Milan, Italy) buffer (pH 3.6) with 10 mM 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) (VWR International, Milan, Italy) and 20 mM FeCl₃ (VWR International, Milan, Italy) in a 8:1:1 (v/v/v) ratio [85 (link)]. The assay protocol was optimized to monitor the bathochromic shift using a microplate reader, as previously described [3 (link)]. Briefly, 170 µL of the FRAP reaction mixture was added to 30 µL of ethanolic extract. After vigorous shaking, the 96-well plate was incubated at 37 °C for 4 min, and the absorbance of each well was recorded at 593 nm. For estimating the FRAP values, Trolox was used as a reference standard in an external calibration curve (y = 7.64x + 0.06; R²: 0.9999; LOD: 1.12·10⁻³ mg/mL; LOQ: 3.38·10⁻³ mg/mL). FRAP was then expressed as mmol TE per 100 g of FW ± SD.

Mannino G., Ricciardi M., Gatti N., Serio G., Vigliante I., Contartese V., Gentile C, & Bertea C.M. (2022). Changes in the Phytochemical Profile and Antioxidant Properties of Prunus persica Fruits after the Application of a Commercial Biostimulant Based on Seaweed and Yeast Extract. International Journal of Molecular Sciences, 23(24), 15911.

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HPLC Analysis of Phenolic Compounds

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Methyl alcohol and acetonitrile (Romil Chemical Ltd., Heidelberg, Germany), trifluoroacetic acid TFA (Merck; Darmstadt, Germany) and acetic acid AcOH (Merck) were HPLC grade. Concentrated aqueous hydrochloric acid (HCl) solution (32%) was from Merck. Deionised water was purified on a Milli-Q system (Millipore, Bedford, MA, USA). L(+)-Tartaric acid and ethyl alcohol of analytical grade were provided by Merck. NaH₂PO₄/Na₂HPO₄ 0.1 M solution buffer, which was prepared using monosodium phosphate (Sigma-Aldrich, Steinheim, Germany) and sodium phosphate dibasic (Merck), and sodium hydroxide NaOH (Panreac, Barcelona, Spain). Solution for phloroglucinolysis was prepared using phloroglucinol and l-ascorbic acid 99%+ reagent (Sigma-Aldrich, St. Louis, MO, USA), hydrochloric acid 37% and MeOH with HPLC grade (VWR, Fontenay-sous-Bois, France). Stop solution was prepared using sodium acetate (VWR) and Milli-Q water. Folin-Ciocalteau’s phenol reagent, sodium carbonate 99.5%+ granular and gallic acid monohydrate 98%+ were provided by Sigma-Aldrich. Standards of malvidin-3-O-glucoside (Mv-3-glc) and (+)-catechin were supplied by Extrasynthèse (Genay, France). All solvents used were previously filtered through 0.45 μm nylon membranes (Lida, Kenosha, WI, USA).

Quaglieri C., Prieto-Perea N., Berrueta L.A., Gallo B., Rasines-Perea Z., Jourdes M, & Teissedre P.L. (2017). Comparison of Aquitaine and Rioja Red Wines: Characterization of Their Phenolic Composition and Evolution from 2000 to 2013. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(2), 192.

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Formulation and Characterization of Lipid-Based Drug Delivery Systems

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Capmul MCM (HLB 4.7) was obtained as a gift from Abitec Corporation (Columbus, OH). The composition of Capmul MCM is approximately 60% of medium-chain monoglycerides and 40% of diglycerides, derived from caprylic acid (83%) and capric acid (17%). Danazol was donated by Sanofi-Aventis Pharmaceutics (Bridgewater, NJ). Caprylic acid (HLB 1), indomethacin, haloperidol, hydrochloric acid (1 N), potassium chloride, potassium phosphate monobasic, sodium hydroxide, sodium acetate, acetic acid, and polyethylene glycol (PEG) 400 were purchased from VWR (Solon, OH). Cremophor RH40 (HLB 14–16) was supplied by BASF Corporation (Tarrytown, NJ). All chemicals and solvents were of analytical purity or high performance liquid chromatography (HPLC) grade and used as received with no further treatment.

Li S., Madan P, & Lin S. (2016). Effect of ionization of drug on drug solubilization in SMEDDS prepared using Capmul MCM and caprylic acid. Asian Journal of Pharmaceutical Sciences, 12(1), 73-82.

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Heparin Pyridinium Salt Functionalization

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Heparin sodium salt (Sigma) was reconstituted with distilled, deionized water (ddH₂O) at 10mg/mL and desalted using Dowex 50WX4 resin (mesh size 100-200, Sigma), adding pyridine until the pH reached a value of ∼6, as previously described [28 (link)]. The solution was reduced and excess pyridine was eliminated using a rotary evaporator (Buchi) before being flash frozen and lyophilized to yield heparin pyridinium salts. The heparin pyridinium salts were then dissolved in solution of 90% DMSO/10% ddH₂O (v/v) at a concentration of 1mg/mL. The solution was mixed for 2h at 50°C in a rotary evaporator and then cooled on ice and precipitated by an equal volume of 95% ethanol (VWR) saturated with sodium acetate (VWR). The precipitate was centrifuged and washed with ethanol prior to dissolving in distilled water (dH₂O). The solution was dialyzed for 3 days with daily exchanges of dH₂O and then lyophilized (Labconco). Finally, the product was functionalized using a 8.0 molar excess of of N-(3-aminopropyl) methacrylamide hydrochloride (APMAm, Polysciences), N-hydroxysuccinimide (NHS, Acros Organics) and N-3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC, Sigma) at an acidic pH. After functionalization, the solution was dialyzed and lyophilized again as described previously. The finished product was stored at -20°C until use.

Krieger J., Ogle M., McFaline-Figueroa J., Segar C., Temenoff J, & Botchwey E. (2015). Spatially localized recruitment of anti-inflammatory monocytes by SDF-1α-releasing hydrogels enhances microvascular network remodeling. Biomaterials, 77, 280-290.

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Formulation and Characterization of DPV-LNG Silicone Delivery System

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Micronised DPV was supplied by Ajinomoto OmniChem n.v. (Wetteren, Belgium). Non-micronised LNG (nmLNG) was supplied by CHEMO Group (Industriale Chimica s.r.l., Saronno, Italy). A custom silicone addition-cure elastomer formulation – formally DEV SILB BIO LSR D1XX-TB Lot 07117–14, a modification of the commercially available Silbione™ LSR D135-QB – was supplied by Elkem (Elkem Silicones, NJ, USA). Potassium dihydrogen orthophosphate, sodium acetate and sodium hydroxide were purchased from VWR International Ltd. (Dublin, Ireland). HPLC-grade acetonitrile and acetone, phosphoric acid (85% w/w in water) and Kolliphor® HS 15 were purchased from Sigma-Aldrich (Gillingham, UK). A Millipore Direct-Q 3 UV Ultrapure Water System (Watford, UK) was used to obtain HPLC-grade water.

Murphy D.J., Dallal Bashi Y.H., McCoy C.F., Boyd P., Brown L., Martin F., McMullen N., Kleinbeck K., Dangi B., Spence P., Hansraj B., Devlin B, & Malcolm R.K. (2022). In vitro drug release, mechanical performance and stability testing of a custom silicone elastomer vaginal ring releasing dapivirine and levonorgestrel. International Journal of Pharmaceutics: X, 4, 100112.

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SCFA Quantification in Cecal Contents

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Caecal contents were homogenized with MilliQ water in a proportion of 1:1.5 (weight:volume) and centrifuged at 12,000 g for 10 min. Supernatants were then filtered through a 0.45 µm Nylon filter (VWR^® Syringe Filters; VWR, Houston, TX, USA) and analyzed by high-performance liquid chromatography (HPLC) as reported in detail elsewhere (Campos et al., 2012 (link); Garcia-Mazcorro, Mills & Noratto, 2016a (link)). Butyric acid, methyl-Butyric acid, caproic acid, sodium acetate, sodium propionate, and valeric acid were purchased from VWR and used as standards to quantify their caecal contents based on retention time and area of peaks at λ = 220 nm.

Garcia-Mazcorro J.F., Lage N.N., Mertens-Talcott S., Talcott S., Chew B., Dowd S.E., Kawas J.R, & Noratto G.D. (2018). Effect of dark sweet cherry powder consumption on the gut microbiota, short-chain fatty acids, and biomarkers of gut health in obese db/db mice. PeerJ, 6, e4195.

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