Human colon adenocarcinoma Caco-2 cell line (DSMZ ACC 169) was cultured in Dulbecco′s Modified Eagle′s Medium (DMEM, Gibco™) supplemented with 20% (v/v) Fetal Bovine Serum (FBS, Gibco™), 1% (v/v) MEM non-essential amino acids (Biowest), and 1% (v/v) Pen-Strep-Amp B (AB, Biological Industries, Cromwell, CT, USA), hereafter referred to as complete DMEM. Cells were incubated at 37 °C in humidified conditions with 5% CO2, the medium was renewed biweekly, and cells were subcultivated upon reaching 80% confluency. Cell passage numbers ranging between 6 and 30 were used for these assays.
The heterogenous plurihormonal cell line STC-1 (intestinal secretin tumor, CRL-3254 ™) was cultured with Dulbecco′s Modified Eagle′s Medium (DMEM) supplemented with 10% (v/v) Fetal Bovine Serum (FBS, Gibco™), and 1% AB (v/v). Cells were grown and maintained at 5% CO2 at 37 °C with medium renewal every 2 to 3 days. Cells were passaged when cell confluency reached 70%, and the passage number used for this assay was between 6 and 34. All mammalian cell work was conducted using sterile technique on LAF benches.
The heterogenous plurihormonal cell line STC-1 (intestinal secretin tumor, CRL-3254 ™) was cultured with Dulbecco′s Modified Eagle′s Medium (DMEM) supplemented with 10% (v/v) Fetal Bovine Serum (FBS, Gibco™), and 1% AB (v/v). Cells were grown and maintained at 5% CO2 at 37 °C with medium renewal every 2 to 3 days. Cells were passaged when cell confluency reached 70%, and the passage number used for this assay was between 6 and 34. All mammalian cell work was conducted using sterile technique on LAF benches.