Microcentrifuge tube

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Microcentrifuge tubes are small, conical-shaped containers designed for use in laboratory centrifugation processes. They are typically made of durable plastic materials and available in various sizes, typically ranging from 0.5 mL to 2.0 mL. The primary function of microcentrifuge tubes is to hold and protect small sample volumes during high-speed centrifugation, enabling the separation and isolation of different components within the sample.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Low protein-binding microcentrifuge tubes (7 citations) 1.5 mL microcentrifuge tubes (6 citations) Low retention microcentrifuge tubes (6 citations) 2 mL microcentrifuge tube (4 citations) Nuclease-free microcentrifuge tubes (3 citations) Siliconized Low-Retention Microcentrifuge Tubes (3 citations) Polypropylene microcentrifuge tubes (3 citations) Microcentrifuge tube magnet (2 citations) RNase-free microcentrifuge tube (2 citations) 1.7 mL microcentrifuge tube (2 citations)

45 protocols using microcentrifuge tube

Metabolite Extraction and Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents used for extraction of metabolites and mass spectrometry analysis were of LC/MS-grade as follows: water and isopropyl alcohol from Honeywell Burdick & Jackson (Muskegon, MI, USA); methyl tert-butyl ether from J.T. Baker (Central City, PA, USA); acetonitrile, methanol and formic acid from Fisher Scientific (Fair Lawn, NJ, USA); standards from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Sigma Aldrich (St. Louis, MO, USA); glass pipette tips, plastic pipette tips and microcentrifuge tubes from Fisher Scientific (Fair Lawn, NJ, USA); Pyrex glass culture tubes from Corning Incorporated (Corning, NY, USA).

Cruickshank-Quinn C., Zheng L.K., Quinn K., Bowler R., Reisdorph R, & Reisdorph N. (2018). Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome. Metabolites, 8(4), 88.

+ Open protocol

+ Expand

Papain-induced Immune Response in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

For dose-response studies, C57BL/6J mice were injected i.p. on day 0 with the indicated dose of papain (EMD Millipore, 5125 Carica papaya) in 0.5 ml PBS. For screening, G3 mice were injected i.p. on day 0 with 0.5 mg papain in 0.5 ml PBS; subcutaneous immunization (0.5 mg papain in 0.2 mL PBS injected over the shoulders) was performed on a small subset of G3 mice (n=1,709) with equivalent effects and screening data were combined with those from i.p. immunizations. On day 15 after immunization, blood was collected in microcentrifuge tubes (Fisher Scientific) for ELISA analysis. G3 mice were injected with ovalbumin (OVA, Sigma-Aldrich, A5503) adsorbed to Alum (InvivoGen, vac-alu-250) in a 1:1 ratio and administered by intramuscular injection; blood was collected 14 days after immunization for ELISA analysis. Intraperitoneal NP-Ficoll immunizations were performed as previously described (36 (link)).

SoRelle J.A., Chen Z., Wang J., Yue T., Choi J.H., Wang K.W., Zhong X., Hildebrand S., Russell J., Scott L., Xu D., Zhan X., Bu C.H., Wang T., Choi M., Tang M., Ludwig S., Zhan X., Li X., Moresco E.M, & Beutler B. (2020). Dominant atopy risk mutations identified by mouse forward genetic analysis. Allergy, 76(4), 1095-1108.

+ Open protocol

+ Expand

Tumor Excision and Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors were excised from euthanized animals at indicated times and frozen in a slurry of dry ice and 70% ethanol. Frozen tumors were homogenized on dry ice and then submitted to three freeze-thawcycles. Protein concentrations were measured by bicinchoninic acid (BCA) assay (Thermo Scientific, Hampton, NH). Additionally, prior to confirmatory euthanasia, blood was collected via cardiac puncture. Blood was collected using a syringe bearing a 22-gauge needle (BD, Franklin Lakes, NJ) and transferred to microcentrifuge tubes (Fisher Scientific, Hampton, NH). IL-6 ELISA of serum and tumor samples was performed per manufacturer’s instruction (R&D Systems, Minneapolis, MN). Serum was run undiluted. Tissue homogenate was run at a concentration of 1.0−1.2 mg/ml at dilutions of 1:1 and 1:2. Final values were adjusted for protein concentration and dilution factors. All samples were run in duplicate.

Davis RW I.V., Papasavvas E., Klampatsa A., Putt M., Montaner L.J., Culligan M.J., McNulty S., Friedberg J.S., Simone CB I.I., Singhal S., Albelda S.M., Cengel K.A, & Busch T.M. (2018). A Preclinical Model to Investigate the Role of Surgically-Induced Inflammation in Tumor Responses to Intraoperative Photodynamic Therapy. Lasers in surgery and medicine, 50(5), 440-450.

+ Open protocol

+ Expand

Metabolite Extraction and LC/MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents used for metabolite extraction and LC/MS analysis were of HPLC or LC/MS-grade. Isopropyl alcohol and water were purchased from Honeywell Burdick & Jackson (Muskegon, Michigan). Methyl tert-butyl ether was purchased from J.T. Baker (Central City, Pennsylvania). Acetonitrile, methanol, chloroform, hexane, and acetic acid were purchased from Fisher Scientific (Fair Lawn, New Jersey).
The neutral and phospholipid standards – Triglyceride d5-(20∶0/20∶1(11Z)/20∶0)), C17 ceramide (d18∶1/17∶0), 1,2-dipentadecanoyl-sn-glycero-3-phosphocholine (15∶0 PC), and 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine (17∶0 PE) – were purchased from Avanti Polar lipids Inc. (Alabaster, Alabama). The aqueous standard – creatinine-d3 – was purchased from Sigma Aldrich (St. Louis, MO).
Solid phase extraction (SPE) 12-position vacuum manifold and Strata NH₂ (55 µM, 70 Å) 100 mg/mL SPE cartridges were purchased from Phenomenex (Torrance, California). Glass pipette tips, plastic pipette tips and microcentrifuge tubes were purchased from Fisher Scientific (Fair Lawn, New Jersey). Pyrex glass culture tubes were purchased from Corning Incorporated (Corning, New York).

Cruickshank-Quinn C.I., Mahaffey S., Justice M.J., Hughes G., Armstrong M., Bowler R.P., Reisdorph R., Petrache I, & Reisdorph N. (2014). Transient and Persistent Metabolomic Changes in Plasma following Chronic Cigarette Smoke Exposure in a Mouse Model. PLoS ONE, 9(7), e101855.

+ Open protocol

+ Expand

Chemical Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

α,α’-Dibromo-ortho-xylene, α,α’-dibromo-meta-xylene, α,α’-dibromo-para-xylene, triethylamine, α-cyano-4-hydroxycinnamic acid (CCA), thymidine, nucleobases, Acyclovir, 2-phenylethanol, 1-butanol, 4-phenyl-1-butanol, deoxyribose, benzoic acid, bisphenol A, trifluoroacetic acid (TFA), and 17β-estradiol (E2) were from Sigma (St. Louis, MO). o-Xylene-d₁₀, 99.3 atom% D, was from CDN Isotopes (Quebec, Canada). Bromine, 99.8%, was from Alfa Aesar (Ward Hill, MA). The Phenol Calibration Mix (12 components, Cat. No. 31694) was from Restek (Bellefonte, PA). Microcentrifuge tubes, pipette tips, ethyl acetate (certified ACS) and HPLC grade acetonitrile (ACN) were from Fisher Scientific (Pittsburgh, PA). All materials were used as received. CCA Matrix Solution was 5 mg/mL in 50% ACN.

Wang P., Zhang Q., Yao Y, & Giese R.W. (2015). Cationic Xylene Tag for Increasing Sensitivity in Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 26(10), 1713-1721.

+ Open protocol

+ Expand

Plasma Amino Acid Analysis in Cows

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples from coccygeal artery and the subcutaneous abdominal vein were collected from each cow for two consecutive days at approximately 07:00, 15:00, and 22:00. Blood was immediately put on ice until centrifugation (3,000×g) at 4°C for 15 min), and the plasma was stored at −20°C for later analysis. The pooled plasma was analyzed for AA by previously described methods [14 (link)]. Briefly, an aliquot of 1 mL of plasma was deproteinized with 10% sulfosalicylic acid (1:1, plasma to 10% sulfosalicylic acid). Samples were then centrifuged at 10,000×g at 4°C for 15 min. The supernatant was filtered through 0.45 μm and 0.22 μm nylon syringe filter units (Fisher Scientific, Pittsburgh, PA, USA) and placed in microcentrifuge tubes (Fisher Scientific, USA). Before analysis, milk was hydrolyzed by adding 6 N HCl and incubating at 110°C for 24 h [11 ]. The AA concentrations of plasma and milk were analyzed using an automatic AA analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan).

Xie Y., Miao C., Lu Y., Sun H, & Liu J. (2021). Nitrogen metabolism and mammary gland amino acid utilization in lactating dairy cows with different residual feed intake. Animal Bioscience, 34(10), 1600-1606.

+ Open protocol

+ Expand

Metabolomics Sample Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse cecal contents and fecal samples were weighed in microcentrifuge tubes (Fisherbrand cat. # 02-682-558) containing glass beads (Sigma cat. # G1145, 150-212 μm, 40 ± 4 mg) and internal standards (ISTD). Then the samples were mixed with extraction solution (100% methanol at 1:9 ratio or 75% acetonitrile/25% methanol at 1:3 ratio) and homogenized with a mixer mill (RETSCH MM400) at 4 °C, 25/s, for 30 min, then centrifuged at 13,000g for 5 min at 4 °C. Supernatants were collected for the following sample preparations, either derivatization or direct dilution, before subjecting to LC-MS analysis.
Liquid samples (culture supernatants, human plasma samples, mouse urine and plasma samples) were first mixed with internal standard (ISTD) in a V-bottom, polypropylene 96-well plate, and then extracted by mixing with extraction solution (100% methanol at 1:9 ratio or 75% acetonitrile/25% methanol at 1:3 ratio). The plate was covered with a lid and centrifuged at 5,000g for 15 min at 4 °C. Supernatant was collected for the following sample preparations, either derivatization or direct dilution, before subjecting to LC-MS analysis.
For cell lysates in DMSO (from ATP assay), sample supernatants were mixed with ISTD, and then diluted in LC-MS water (total 4.5-fold dilution) before LC-MS analysis.

Liu Y., Chen H., Van Treuren W., Hou B.H., Higginbottom S.K, & Dodd D. (2022). Clostridium sporogenes uses reductive Stickland metabolism in the gut to generate ATP and produce circulating metabolites. Nature microbiology, 7(5), 695-706.

+ Open protocol

+ Expand

Metabolomics Sample Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Photochemical Crosslinking of Oligonucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oligonucleotides (1), (2) and (3) (shown in Scheme 1) were acquired from Integrated DNA Technologies. All three were mixed together in aqueous 500 mM sodium phosphate at pH 7.0 with the psoralen oligonucleotide (1) at 1 mM and the other two Oligonucleotides at 1.1 mM. The mixture was then briefly heated to 95 °C followed by slow cooling to room temperature to allow hybridization to occur. Ten microliters aliquots were then cooled to 4 °C and irradiated in 1.5 ml polypropylene microcentrifuge tubes (Fisher Scientific, 02-682-550) with UV light at 365 nm using a UVL-21 compact UV lamp (UVP). Aliquots of 1 uL were taken over a time-course and were analyzed by denaturing PAGE. The gel was visualized and photographed using the UV shadowing of a fluorescent TLC plate.

Litovchick A., Clark M.A, & Keefe A.D. (2014). Universal strategies for the DNA-encoding of libraries of small molecules using the chemical ligation of oligonucleotide tags. Artificial DNA, PNA & XNA, 5, e27896.

+ Open protocol

+ Expand

Plasma Amino Acid and Glucose Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The plasma AA concentration was analyzed with norleucine as an internal standard in an Automatic AA Analyzer (Hitachi High-technologies Corporation, Tokyo, Japan). To measure the plasma glucose levels, plasma was pretreated with ice-cold sulfosalicylic acid (50 g/L) at a ratio of 1:4 (v/v) to precipitate protein^{24 (link)}, followed by centrifugation at 8,320 × g for 30 min at 4 °C. The supernatant was filtered through 0.45-μm and 0.22-μm nylon syringe filter units (Fisher Scientific, Pittsburgh, PA) and placed in microcentrifuge tubes (catalog no. 05-664-34, Fisher Scientific). The plasma glucose concentrations were subsequently measured using an Auto Analyzer 7020 instrument (Hitachi High-technologies) and commercial colorimetric kit (DiaSys Diagnostics Systems GmbH, Frankfurt, Germany)^{25 (link)}.

Wang B., Sun H., Wu X., Jiang L., Guan L.L, & Liu J. (2018). Arteriovenous blood metabolomics: An efficient method to determine the key metabolic pathway for milk synthesis in the intra-mammary gland. Scientific Reports, 8, 5598.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!