Dulbecco s modified eagle s medium dmem

Manufactured by Cell Signaling Technology

Sourced in United States

Dulbecco's Modified Eagle's Medium (DMEM) is a cell culture medium formulation that provides the necessary nutrients and components for the growth and maintenance of a variety of cell lines in vitro. It is a widely used basal medium that supports the proliferation and survival of diverse cell types.

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2 protocols using dulbecco s modified eagle s medium dmem

Colchicine-Induced Apoptosis Pathway

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Purified Colchicine (99.89%), MTT, and DMSO were purchased from Sigma–Aldrich Co. (St. Louis, MO, U.S.A.). Antibodies against Bcl-2, Bax, cleaved-caspase-3, p53, Rb, phospho Rb (pRb), β-actin and horseradish peroxidase (HRP) secondary conjugated antibodies were obtained from Santa Cruz Biotechnology, Inc., (Santa Cruz, CA, U.S.A.). Dulbecco’s Modified Eagle’s Medium (DMEM), RPMI 1640, fetal bovine serum (FBS), penicillin, and streptomycin were obtained from Cell Signaling Technology (Danvers, MA, U.S.A.). Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and propidium iodide (PI) were purchased from BD Bioscience (San Jose, CA, U.S.A.).

Yan L., Huang H., Zhang Y., Yuan X., Yan Z., Cao C, & Luo X. (2020). Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells. Bioscience Reports, 40(3), BSR20194065.

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Investigating the mTOR Signaling Pathway

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PBS buffer, Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum, as well as anti-mTOR, anti-Akt, anti-p70S6K, anti-p-mTOR (Ser 2448), anti-p-Akt (Ser473), and anti-p-p70S6K (Thr389) antibodies were obtained from Cell Signaling Technology (MA, USA). Dimethyl sulfoxide (DMSO), protease inhibitor cocktail, and rabbit anti-human β-actin antibodies were obtained from Sigma (St. Louis, MO, USA). PCR kit, Trizol, and first-strand cDNA synthesis kit were purchased from TAKARA Company (Dalian, China). Polyvinylidene difluoride (PVDF) membranes and goat anti-rabbit and horseradish peroxidase-conjugated IgG were obtained from Millipore (MA, USA). TRIzol reagent and sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) gels were acquired from Beyotime Biotechnology (Haimen, China).

Li Z., Wang B., Tang L., Chen S, & Li J. (2016). Quinazoline derivative compound (11d) as a novel angiogenesis inhibitor inhibiting VEGFR2 and blocking VEGFR2-mediated Akt/mTOR /p70s6k signaling pathway. Iranian Journal of Basic Medical Sciences, 19(4), 411-416.

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