1200l mass spectrometer

Manufactured by Agilent Technologies

The 1200L mass spectrometer is a laboratory instrument designed for the detection and analysis of chemical compounds. It utilizes mass spectrometry technology to accurately measure the mass-to-charge ratio of ionized molecules, providing detailed information about their molecular composition.

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Lab products found in correlation

Fluostar omega, by BMG Labtech (1 mentions) Mercury spectrometer, by Agilent Technologies (1 mentions) 1100 series hplc, by Agilent Technologies (1 mentions) C18 guard column, by Phenomenex (1 mentions) Spectramax m5, by Molecular Devices (1 mentions) Amx 400 mhz spectrometer, by Bruker (1 mentions)

4 protocols using 1200l mass spectrometer

UV/Vis Characterization and Light Activation

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All
materials that were purchased from commercial sources were used without
any further purification. All ¹H NMR were obtained on a
Varian mercury spectrometer (400 MHz), and chemical shifts are reported
relative to the residual solvent peak of CD₃CN (δ
1.94). Electrospray ionization (ESI) mass spectra were obtained on
a Varian 1200L mass spectrometer at the Environmental Research Training
Laboratory (ERTL) at the University of Kentucky. The UV/vis absorption
spectra were obtained on a BMG Labtech FLUOstar Omega microplate reader.
Light activation experiments were performed using a 470 nm LED array
from Elixa (for photochemistry and DNA gels) or with >450 nm light
using the Indigo LED (for cytotoxicity experiments). The Prism software
package was used to analyze the data.

Havrylyuk D., Heidary D.K., Sun Y., Parkin S, & Glazer E.C. (2020). Photochemical and Photobiological Properties of Pyridyl-pyrazol(in)e-Based Ruthenium(II) Complexes with Sub-micromolar Cytotoxicity for Phototherapy. ACS Omega, 5(30), 18894-18906.

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Characterization of Ruthenium(II) Bipyridine Complex

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All chemicals were of reagent grade and were used without further purification. cis-Dichlorobis(2, 2′-bipyridine)ruthenium(II) dihydrate was purchased from Strem Chemicals. Custom DNA sequences were purchased from Eurofins. All ¹H-NMR and ¹³C-NMR were obtained on a Varian Mercury spectrometer (400, 100 MHz). The ¹H chemical shifts are reported relative to the residual solvent peak of CD₃CN at δ 1.94. The ¹³C chemical shifts are referenced to CD₃CN at δ 1.39. Electrospray ionization (ESI) mass spectra were obtained on a Varian 1200L mass spectrometer at the Environmental Research Training Laboratory (ERTL) at the University of Kentucky. Luminescence spectra were obtained on a Molecular Devices Spectramax M5 microplate reader. Circular dichroism (CD) experiments were performed on a Jasco J-815 CD Spectrometer equipped with an MPTC-490S/15 temperature controller. HPLC experiments were run on an Agilent 1100 Series HPLC equipped with a model G1311A quaternary pump, G1315B UV diode array detector and Chemstation software version B.01.03. Chromatographic conditions were optimized on a Column Technologies Inc. C18 120 Å column fitted with a Phenomenex C18 guard column. The Prism software package was used to analyze kinetic data with a single exponential equation and luminescence spectra with area under the curve.

Wachter E., Moyá D, & Glazer E.C. (2016). Combining a Ru(II) “Building Block” and Rapid Screening Approach to Identify DNA Structure-Selective “Light Switch” Compounds. ACS combinatorial science, 19(2), 85-95.

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Structural Elucidation of Bioactive Compound

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The active compound of fermentation supernatant of GN59 was subjected to NMR and LC–MS analysis to obtain the chromatogram and the prospective mass spectra of the separated compound. NMR spectra was recorded (¹H NMR 400 MHz and ¹³C NMR 100 MHz) on Bruker AMX400MHz Spectrometer (Bruker BioSpin GmbH, Ettlingen, Germany) using deuterated chloroform (CDCl₃) as solvent. Mass spectrum was carried out using Varian 1200 L Mass Spectrometer (Varian India Pvt. Ltd., Powai, Mumbai).

Wang Y., Zhao B., Liu Y., Mao L., Zhang X., Meng W., Liu K, & Chu J. (2020). A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities. AMB Express, 10, 6.

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Steroid Extraction and Quantification

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Steroids were extracted from cell media using ChemElute cartridges (Varian) and eluted from the cartridge with dichloromethane. The eluates were evaporated to dryness and reconstituted in 10% acetonitrile. Identification and quantification of steroid products was achieved by tandem mass spectrometry using a Varian 1200 L mass spectrometer with a triple quadrupole detector [25 ].

Robertson S., Diver L.A., Alvarez-Madrazo S., Livie C., Ejaz A., Fraser R., Connell J.M., MacKenzie S.M, & Davies E. (2017). Regulation of Corticosteroidogenic Genes by MicroRNAs. International Journal of Endocrinology, 2017, 2021903.

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