QC and 5-FU were purchased from Sigma-Aldrich (St. Louis, MO, USA). CHX (cycloheximide) and MG132 were purchased from Calbiochem (Darmstadt, Hesse, Germany). Primary antibodies against the following proteins were used: Nrf2, phospho-p38, p38, phospho-ERK, ERK, phospho-JNK, JNK, Keap1, Cul3 (all from Cell Signaling Technology, Beverly, MA, USA), β-actin (Sigma-Aldrich, St. Louis, MO, USA), phospho-γH2AX (Millipore, Darmstadt, Hesse, Germany) and HIF-1α (R&D Systems, Minneapolis, MN, USA). The secondary antibodies used for immunoblotting included horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit (Cell Signaling Technology, Beverly, MA, USA).
Phospho γh2ax
Manufactured by Merck Group
Sourced in United States
Phospho-γH2AX is a laboratory reagent used in the detection and quantification of DNA double-strand breaks. It functions by specifically binding to the phosphorylated form of the histone protein H2AX, which is rapidly induced in response to DNA damage.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p38, by Cell Signaling Technology (1 mentions)
Hif 1α, by R&D Systems (1 mentions)
Phospho erk, by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Keap1, by Cell Signaling Technology (1 mentions)
Ab8580, by Abcam (1 mentions)
Ab1791, by Abcam (1 mentions)
Ab9045, by Abcam (1 mentions)
Ab8898, by Abcam (1 mentions)
Ab1220, by Abcam (1 mentions)
Nbs1 nb100 143, by Novus Biologicals (1 mentions)
Atr n 19, by Santa Cruz Biotechnology (1 mentions)
Mre11 nb100 142, by Novus Biologicals (1 mentions)
2 protocols using phospho γh2ax
1
Analyzing Nrf2 and Stress Signaling
2
Histone Modifications and DNA Damage Signaling
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