Rbk 004 rapid barcoding kit

Genomic DNA was extracted from bacterial colonies that were harvested from chocolate blood agar plates that had been cultured for 16 h at 37°C and 5% CO₂ using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. Libraries for DNA sequencing were prepared with the RBK-004 Rapid Barcoding Kit (Nanopore, Oxford, United Kingdom). DNA sequencing was performed on a Nanopore Mk1C with an R9.4.1 Flow Cell for 24 h. The resulting read files were base-called, and genomes were assembled and confirmed as H. influenzae using Epi2me-labs wf-bacterial-genomes/0.2.12 with the standard setting (Nanopore). The multilocus sequence type (MLST) of each assembled genome was determined according to PubMLST (https://pubmlst.org) and assemblies were predicted to an H. influenzae serotype based on the cap locus using hicap/1.0.3 (11 (link)).

Genomic DNA was extracted from bacterial colonies that were harvested from chocolate blood agar plates that had been cultured for 16 h at 37°C and 5% CO₂ using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. Libraries for DNA sequencing were prepared with the RBK-004 Rapid Barcoding Kit (Nanopore, Oxford, United Kingdom). DNA sequencing was performed on a Nanopore Mk1C with an R9.4.1 Flow Cell for 24 h. The resulting read files were base-called, and genomes were assembled and confirmed as H. influenzae using Epi2me-labs wf-bacterial-genomes/0.2.12 with the standard setting (Nanopore). The multilocus sequence type (MLST) of each assembled genome was determined according to PubMLST (https://pubmlst.org) and assemblies were predicted to an H. influenzae serotype based on the cap locus using hicap/1.0.3 (11 (link)).