The cells were centrifuged at 1000 rpm for 5 min, and the growth medium was removed. The pellet was washed in PBS twice and centrifuged again. The pellet was resuspended in Milli-Q water plus protease inhibitor and sonicated in ice twice for 10-s, with a 30-s break to prevent the warming, using the Microson XL Model DU-2000 (Misonix Inc., New York, NY, USA). The total protein content was estimated by the Bradford method [30 (link)].
Microson xl model du 2000
Manufactured by Bioventus
Sourced in United States
The Microson XL Model DU-2000 is a laboratory equipment designed for ultrasonic homogenization and cell disruption. It generates high-frequency vibrations that can effectively disrupt cellular structures and mix samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using microson xl model du 2000
1
Protein Extraction and Quantification
2
Protein Quantification of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cultured cells were centrifuged at 1000 rpm for 5 min to remove the growth medium. The pellet was washed in PBS, resuspended in Milli-Q water, and sonicated twice for 10 s in ice, with a 30 s break to prevent mixture warming, using the Microson XL Model DU-2000 (Misonix Inc. Farmingdale, NY, USA). Total protein content was estimated using the Bradford method [49 (link)].
3
Protein Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were centrifuged at 1000 rpm for 5 min, and the growth medium was removed. The pellet was washed in PBS twice and centrifuged again. Pellet was resuspended in Milli-Q water plus protease inhibitor and sonicated in ice twice for 10-s, with a 30-s break to prevent the mixture from warming, using the Microson XL Model DU-2000 (Misonix Inc., Farmingdale, NY, USA). Total protein content was estimated with the Bradford method [31 (link)].
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