The microscopy setup consisted of an epifluorescence microscope (Ti-E, Nikon), an objective lens (40× CFI Plan Apo Lambda, 0.95 NA, Nikon), a relay optics box for dual-color imaging (GA03; G-Angstrom, Japan), and an electron multiplier-type CCD camera (EM-CCD, iXon DV887 or DU897; Andor Technology PLC, UK). A slit was placed at the imaging surface of the microscope in the relay optics. A dichroic mirror (FF458-Di02, Semrock) located just outside the slit split the optical pathway after the imaging surface into two pathways for cyan (CFP) and yellow (YFP1G) fluorescence. The two pathways converged on the acceptance surface of the EM-CCD camera side by side. Band-pass filters were set for each pathway (467–499 nm for CFP and 510–560 nm for YFP). Multiphoton fluorescence recovery after photobleaching (MP-FRAP) experiments were conducted as described elsewhere [17 (link)]. Image analyses were carried out in the ImageJ software (NIH, USA).
Ff458 di02
Manufactured by IDEX Corporation
Sourced in United States
The FF458-Di02 is a compact and versatile flow cell designed for use in various laboratory applications. It features dual inlets and a transparent housing, enabling visual observation of fluid flow. The core function of this product is to facilitate the controlled introduction and monitoring of liquids or gases within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ixon dv887, by Oxford Instruments (1 mentions)
Du897, by Oxford Instruments (1 mentions)
Plan apo, by Nikon (1 mentions)
Intensilight c hgfi, by Nikon (1 mentions)
Ixon3, by Oxford Instruments (1 mentions)
Na water immersion objective, by Olympus (1 mentions)
Fvmpe rs, by Olympus (1 mentions)
Ff01 520 35, by IDEX Corporation (1 mentions)
Ff01 400 40, by IDEX Corporation (1 mentions)
Insight ds, by Spectra-Physics (1 mentions)
Maitai deepsee, by Spectra-Physics (1 mentions)
3 protocols using ff458 di02
1
Dual-color FRAP Microscopy Setup
2
FRET Microscopy Imaging Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FRET images were captured on an inverted microscope (Ti; Nikon) equipped with a 40×/NA Plan Apo objective (Nikon), cooled charge-coupled device camera (ANDOR iXon3), and 130-W mercury lamp (Intensilight C-HGFI; Nikon) with an FF01-442 excitation filter, an FF458-Di02 dichroic mirror, and emission filters (FF01-483 for enhanced cyan fluorescent protein and FF01-520 for Venus; Semrock, Rochester, NY).
3
Multimodal Cartilage Imaging in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following staining, anesthetized mice were moved onto the stage of a multi-photon microscope (FVMPE-RS, Olympus, Japan). Medial femoral condyle cartilage was imaged using a 25 × 1.05 NA water-immersion objective (Olympus Inc., Japan) coupled with two independent multi-photon infrared pulsed lasers (InSight DS and Mai Tai DeepSee, Spectra Physics Inc., USA) enabling simultaneous excitation at different wavelengths. The first laser was tuned to 800 nm to produce SHG while the second laser was tuned to 940 nm to excite both live and dead cell stains. The emission signals were directed to a single-edge dichroic beam splitter (FF458-Di02, Semrock inc., USA) to separate the SHG signal from the live/dead cell signal. Live and dead cell signals were further separated using a dichroic beam splitter (FF570-Di01, Semrock inc. USA) and were then focused onto two non-descanned detectors through two single bandpass filters, FF01-520/35 and FF01-612/69 (Semrock inc., USA) to capture the live and dead cell signals respectively. The SHG signal was directed to a single-band bandpass filter centered at 400 nm (FF01-400/40, Semrock inc., USA) prior to focussing it onto a sensitive GaAsp non-descanned detector.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!