Fasting maternal venous blood samples were obtained on the day of electronic impedance cardiography monitoring. Samples were centrifuged, and supernatants were stored at − 80 °C. TSH was measured using an Elecsys-TSH kit (Roche Diagnostics, Mannheim, Germany, RRID:AB_2756377). FT4 was measured using an FT4 Flex reagent (Siemens, Berlin, Germany, RRID:AB_2801666). Thyroid peroxidase antibody was measured with an E-Anti-TPO kit (Roche Diagnostics, Mannheim, Germany, RRID:AB_10698637) and was graded as positive when greater than 60 IU/ml. The normal reference for TSH in the third trimester was 0.3 to 3.5 mIU/L, as recommended [21 (link)].
E anti tpo kit
Manufactured by Roche
Sourced in Germany
The E-Anti-TPO kit is a lab equipment product used for the quantitative determination of anti-thyroid peroxidase (anti-TPO) antibodies in human serum or plasma. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) method to detect the presence and measure the concentration of anti-TPO antibodies in the sample.
Automatically generated - may contain errors
Lab products found in correlation
E tsh kit, by Roche (5 mentions)
E free t4 kit, by Roche (4 mentions)
Icp ms, by PerkinElmer (4 mentions)
Automatic analyzer 7600, by Hitachi (2 mentions)
Elecsys tsh kit, by Roche (1 mentions)
Cobas 8000, by Roche (1 mentions)
Cobas 8000 e602, by Roche (1 mentions)
Elecys insulin, by Roche (1 mentions)
Electrochemiluminescence immunoassay, by Roche (1 mentions)
Cobas e801, by Roche (1 mentions)
6 protocols using e anti tpo kit
1
Maternal Thyroid Function Monitoring
2
Thyroid Disease Epidemiology Survey
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Data on demographic characteristics, personal medical history, and family history of thyroid diseases were collected by interview during the survey. Life style risk factors (i.e., smoking status) were based on self-reporting. Serum TSH, FT4, and TPOAb concentrations were measured by an electrochemiluminescence immunoassay. Serum TSH was measured using an E-TSH kit (Roche Diagnostics, Mannheim, Germany). Serum FT4 was measured by E-Free T4 kit (Roche Diagnostics, Mannheim, Germany). Serum TPOAb was measured by E-Anti-TPO kit (Roche Diagnostics, Mannheim, Germany). The results of TSH, FT4, and TPOAb met the specifications regarding accuracy, general chemistry, special immunology, and ligand of the quality control and quality assurance program of the College of American Pathologists. UI was measured using an inductively coupled plasma mass spectrometry device (ICP-MS; Perkin Elmer ICP-MS, Waltham, MA, USA). The laboratory that measured UI is enrolled in the “Ensuring the Quality of Urinary Iodine Procedures (EQUIP)” quality assurance program run by the Centers for Disease Control of the United States of America [17 ].
3
Thyroid and Lipid Profile Measurements
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Blood samples were obtained from each participant’s antecubital vein in the morning after fasting for at least eight hours. As previously reported, serum TSH, free T4, and thyroid peroxidase antibody (TPOAb) were measured with an electrochemiluminescence immunoassay (Cobas8000 E-602/Roche Diagnostics, Mannheim, Germany)21 (link). Briefly, TSH was measured with an E-TSH kit (Roche Diagnostics), and the TSH reference interval was determined to be between the 2.5th and 97.5th percentile of the serum TSH levels of the reference population, as previously reported21 (link). Serum free T4 was measured using an E-Free T4 kit (Roche Diagnostics), and the reference range was 0.89–1.76 ng/mL. TPOAb was measured using an E-Anti-TPO kit (Roche Diagnos-tics); the normal range for TPOAb in humans is < 34.0 IU/mL.
Lipid profiles were measured with a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan) using commercially available kits (Sekisui, Osaka, Japan). Serum total cholesterol and triglycerides were measured by enzymatic methods. Insulin was measured by electrochemiluminescence immunoassay (Cobas 8000/Roche/Germany) using Elecys/insulin (Roche/Germany).
Lipid profiles were measured with a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan) using commercially available kits (Sekisui, Osaka, Japan). Serum total cholesterol and triglycerides were measured by enzymatic methods. Insulin was measured by electrochemiluminescence immunoassay (Cobas 8000/Roche/Germany) using Elecys/insulin (Roche/Germany).
4
Thyroid Function and Iodine Status Analysis
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For the analysis of serum TSH, fT4, and TPOAb approximately 15 mL of blood was collected. After separation of the serum within 30 minutes, the sample was transferred to the testing facility. Collected specimens were analyzed by an electrochemiluminescence immunoassay within 24 hours.
Serum TSH, fT4 and TPOAb levels were measured with an electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany). TSH was measured using an E-TSH kit (Roche Diagnostics), for which the reference range was 0.35 to 5.50 mIU/L. The fT4 was measured using a E-Free T4 kit (Roche Diagnostics), the reference range was 0.89 to 1.76 ng/mL. TPOAb was measured using an E-Anti-TPO kit (Roche Diagnostics); the normal range for TPOAb in humans is <34.0 IU/mL. The reported results of TSH, fT4, and TPOAb met the specifications regarding accuracy, general chemistry, special immunology and ligand of the quality control and quality assurance program of the College of American Pathologist [25 ]. UI concentrations were measured with an inductively coupled plasma mass spectrometry devise (ICP-MS; Perkin Elmer ICP-MS, Waltham, MA, USA). UI concentrations were measured using an Iodine standard (Inorganic Venture, Christiansburg, VA, USA). Iodine concentrations were adjusted using creatinine concentration to correct for variable water excretion rates at the time of spot urine specimen collection.
Serum TSH, fT4 and TPOAb levels were measured with an electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany). TSH was measured using an E-TSH kit (Roche Diagnostics), for which the reference range was 0.35 to 5.50 mIU/L. The fT4 was measured using a E-Free T4 kit (Roche Diagnostics), the reference range was 0.89 to 1.76 ng/mL. TPOAb was measured using an E-Anti-TPO kit (Roche Diagnostics); the normal range for TPOAb in humans is <34.0 IU/mL. The reported results of TSH, fT4, and TPOAb met the specifications regarding accuracy, general chemistry, special immunology and ligand of the quality control and quality assurance program of the College of American Pathologist [25 ]. UI concentrations were measured with an inductively coupled plasma mass spectrometry devise (ICP-MS; Perkin Elmer ICP-MS, Waltham, MA, USA). UI concentrations were measured using an Iodine standard (Inorganic Venture, Christiansburg, VA, USA). Iodine concentrations were adjusted using creatinine concentration to correct for variable water excretion rates at the time of spot urine specimen collection.
5
Thyroid Function and Iodine Status
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Blood samples were collected from the antecubital vein after a night of fasting. Samples were centrifuged for at least 30 minutes and transferred to the laboratory. Samples were analyzed within 24 hours of collection at the central lab in Seoul. Levels of TSH, fT4, and TPO-Ab were analyzed using electrochemiluminescence immunoassay. TSH was measured using the E-TSH kit (Roche Diagnostics, Mannheim, Germany) with normal reference values of 0.35–5.50 mIU/L. Free thyroxine was measured using the E-Free T4 kit (Roche Diagnostics, Mannheim, Germany) with normal reference values of 0.89–1.76 ng/mL. TPOAb level was measured using an E-Anti-TPO kit (Roche Diagnostics), and values were considered normal if they were lower than 34.0 IU/mL. Single random urine samples were obtained from 1,034 adolescents aged 10–18 years. UIC was measured by inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer, Waltham, MA, USA) using an iodine standard (Inorganic Venture, Christiansburg, VA, USA) and is reported as μg/L. The reported results met the guidelines regarding accuracy, general chemistry, special immunology, quality control, and quality assurance set forth by the College of American Pathologists.
6
Thyroid and Lipid Biomarker Measurement
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Blood and urine samples were collected from all participants. Serum free T4, TSH, and thyroid peroxidase antibodies (TPOAb) were analyzed with electrochemiluminescence immunoassay. Free T4, TSH, and TPOAb levels were measured using an E- free T4 kit (Cobas e 411, Roche Diagnositics GmbH Mannheim, Germany), E-TSH kit (Cobas e 801, Roche Diagnostics GmbH, Mannheim, Germany), and E-Anti-TPO kit (Cobas e 801, Roche Diagnostics GmbH, Mannheim, Germany). The reference ranges for free T4 and TPOAb were 0.89 to 1.76 ng/mL and 0–34 IU/mL, respectively. As TSH is affected by iodine intake status, and excess iodine is prevalent in Korea [23 (link)], serum TSH levels between 0.62 and 6.68 mIU/L, based on population data [24 (link)], were considered as reference ranges. Urine iodine concentration was measured by inductively coupled plasma mass spectroscopy (ICP-MS: PerkinElmer ICP-MS, Waltham, USA) and was adjusted for creatinine concentration.
Serum total cholesterol, TG, and HDL-cholesterol were measured by enzymatic methods using a commercial kit (Sekisui, Osaka, Japan) with a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). LDL-cholesterol was calculated using the Friedewald formula: LDL-cholesterol = total cholesterol − HDL-cholesterol − (TG/5) [25 (link)]. Non-HDL-cholesterol was calculated by subtracting the quantity of HDL-cholesterol from total cholesterol [26 (link)].
Serum total cholesterol, TG, and HDL-cholesterol were measured by enzymatic methods using a commercial kit (Sekisui, Osaka, Japan) with a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). LDL-cholesterol was calculated using the Friedewald formula: LDL-cholesterol = total cholesterol − HDL-cholesterol − (TG/5) [25 (link)]. Non-HDL-cholesterol was calculated by subtracting the quantity of HDL-cholesterol from total cholesterol [26 (link)].
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