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4 1 1 3 3 tetramethylbutyl phenyl polyethylene glycol triton x100

Manufactured by Merck Group
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4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton-X100) is a non-ionic surfactant commonly used in laboratory applications. It functions as a detergent and emulsifier, aiding in the solubilization and dispersion of various molecules and compounds.

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Lab products found in correlation

Potassium hydrogen phosphate, by Merck Group (2 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (2 mentions) Potassium dihydrogen phosphate, by Merck Group (2 mentions) Tris hydroxymethyl aminomethane tris, by Merck Group (2 mentions) Kojic acid, by Merck Group (1 mentions) Alpha melanocyte stimulating hormone, by Merck Group (1 mentions) L 3 4 dihydroxyphenylalanine, by Merck Group (1 mentions) L dopa, by Merck Group (1 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (1 mentions) 3 isobutyl 1 methylxanthine, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Trolox, by Merck Group (1 mentions) Mushroom tyrosinase, by Merck Group (1 mentions) Polyethylene glycol 6000, by Merck Group (1 mentions) Dioctyl sulfosuccinate, by Merck Group (1 mentions) N octyl β d glucoside, by Merck Group (1 mentions) Polyoxyethylene 20 sorbitan monooleate, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) Cetyltrimethylammonium bromide, by Merck Group (1 mentions) Hplc grade solvent, by Merck Group (1 mentions)

5 protocols using 4 1 1 3 3 tetramethylbutyl phenyl polyethylene glycol triton x100

1

Melanogenesis Inhibition Assay Protocol

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The kojic acid, trolox, l-ascorbic acid, l-4-hydroxyphenylalanine (l-tyrosine), l-3,4-dihydroxyphenylalanine (l-DOPA), alpha-melanocyte stimulating hormone (α-MSH), 3-isobutyl-1-methylxanthine (IBMX), 4-(1,1,3,3-tetramethylbutyl)phenylpolyethylene glycol (Triton™ X-100), phenylmethylsulfonyl fluoride (PMSF), 3-morpholinosydnonimine (SIN-1), dimethyl sulfoxide (DMSO), potassium hydrogen phosphate, potassium dihydrogen phosphate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and mushroom tyrosinase were all purchased from Sigma-Aldrich (St. Louis, MO, USA).
Jeong Y., Hong S., Jung H.J., Ullah S., Hwang Y., Choi H., Ko J., Lee J., Chun P., Chung H.Y, & Moon H.R. (2022). Identification of a Novel Class of Anti-Melanogenic Compounds, (Z)-5-(Substituted benzylidene)-3-phenyl-2-thioxothiazolidin-4-one Derivatives, and Their Reactive Oxygen Species Scavenging Activities. Antioxidants, 11(5), 948.
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2

Immobilization of Burkholderia cepacia Lipase

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Carbon nanofiber (CNF), Burkholderia cepacia lipase (BCL), nitric acid, sulphuric acid, vinyl acetate, toluene, surfactants viz. [4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100), polyoxyethylene (20) sorbitan monooleate, n-octyl-β-d-glucoside (OG), polyethylene glycol 6000 (PEG6000), sodium dodecyl sulphate (SDS), dioctyl sulfosuccinate (DOSS), cetyl trimethyl ammonium bromide (CTAB), merpol], p-nitro-phenyl palmitate (p-NPP), 2-ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich Corporation (St. Louis, MO, USA). HPLC grade solvents were obtained from Merck Ltd.
Soni S., Dwivedee B.P, & Chand Banerjee U. (2018). Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate. RSC Advances, 8(49), 27763-27774.
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3

Liposome Formulations for Biomedicine

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All phospholipids, including egg phosphatidylcholine (EPC), cholesterol (chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (ammonium salt) (mPEG2000-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-3400] (ammonium salt) (MalPEG3400-DSPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD-DOPE) were commercially acquired from Lipoid GmbH (Ludwigshafen, Germany) and Avanti Polar Lipids (Alabama, USA). The detergent Tris (hydroxymethyl)-aminomethane (Tris) and 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton-X100) was purchased from Sigma (Taufkirchen, Germany), and the near infrared fluorescent (NIRF) dye, DY-676-COOH was acquired from DYOMICs GmbH (Jena, Germany).
Tansi F.L., Rüger R., Kollmeier A.M., Rabenhold M., Steiniger F., Kontermann R.E., Teichgräber U.K., Fahr A, & Hilger I. (2020). Targeting the Tumor Microenvironment with Fluorescence-Activatable Bispecific Endoglin/Fibroblast Activation Protein Targeting Liposomes. Pharmaceutics, 12(4), 370.
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4

Lipid Nanoparticle Characterization Protocol

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Phospholipids used were egg phosphatidylcholine (EPC), cholesterol (chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] ammonium salt (mPEG2000-DSPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD-DOPE). All were purchased from Lipoid GmbH (Ludwigshafen, Germany) and Avanti Polar Lipids (Alabaster, AL, USA). The detergent Tris (hydroxymethyl)-aminomethane (Tris) and 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton-X100) was acquired from Sigma (Taufkirchen, Germany), whereas the near-infrared fluorescent (NIRF) dye, DY676-COOH was from DYOMICs GmbH (Jena, Germany).
Tansi F.L., Rüger R., Böhm C., Steiniger F., Raasch M., Mosig A.S., Kontermann R.E., Teichgräber U.K., Fahr A, & Hilger I. (2020). Rapid Target Binding and Cargo Release of Activatable Liposomes Bearing HER2 and FAP Single-Chain Antibody Fragments Reveal Potentials for Image-Guided Delivery to Tumors. Pharmaceutics, 12(10), 972.
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5

Melanogenic Inhibitor Evaluation Protocol

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BPA1–BPA13 were synthesized in our laboratory. Mushroom tyrosinase, 3-(4-hydroxyphenyl)-L-alanine (L-tyrosine), L-DOPA, 5-hydroxy-2-hydroxymethyl-4H-4-pyranone (kojic acid), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), alpha-melanocyte-stimulating hormone (α-MSH), phenylmethylsulfonyl fluoride, 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton™ X-100), dimethyl sulfoxide (DMSO), ethyl alcohol, potassium dihydrogen phosphate, potassium hydrogen phosphate, benzoylacetonitrile, piperidine, sodium acetate, and several benzaldehydes were purchased from Sigma-Aldrich (St Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum, trypsin, phosphate-buffered saline, penicillin, and streptomycin were obtained from Gibco Life Technologies (Carlsbad, CA, USA). All chemicals and solvents were of analytical grade. The B16F10 mouse melanoma cell line was kindly provided by Professor Hae Young Chung (College of Pharmacy, Pusan National University, Republic of Korea).
Yun H.Y., Kim D.H., Son S., Ullah S., Kim S.J., Kim Y.J., Yoo J.W., Jung Y., Chun P, & Moon H.R. (2015). Design, synthesis, and anti-melanogenic effects of (E)-2-benzoyl-3-(substituted phenyl)acrylonitriles. Drug Design, Development and Therapy, 9, 4259-4268.
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