Bovine serum protein bsa

Manganese chloride tetrahydrate (MnCl₂•4H₂O), tetramethylammonium hydroxide solution (TMA, 1.0 M), bovine serum protein (BSA), and 2′,7′-dichloroflourescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich. NH₂-PEG₃₀₀₀ and chlorin e6 (Ce6) were purchased from JenKem Technology and Frontier, respectively. The hydrogen peroxide (H₂O₂, 30 wt.%) solution was purchased from Sinopharm Chemical Reagent Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were bought from KeyGen BioTech and Gibco, respectively. HIF-1α antibody (mouse monoclonal antibody) and FITC-labeled goat anti-mouse IgG were purchased from Beyotime Biotechnology. All solutions were prepared by using ultrapure water (18.2 MΩ).

Cells were seeded on samples at a density of 3 × 10⁴ cells/well. After 1, 4 and 24 h, samples were rinsed with PBS. Then cells were fixed, permeabilized and blocked successively by 4% paraformaldehyde (PFA) diluent, 0.1% (v/v) Triton X-100 (Amresco, USA) and 1 wt % bovine serum protein (BSA, Sigma, USA) respectively. Then FITC phalloidin was added to stain F-actin, and DAPI stain nucleus. Samples were rinsed with PBS after each step. Finally, specimens were observed by confocal laser scanning microscopy (CLSM, Leica SP8, Germany).

The blueberry anthocyanins were extracted from Rabbiteye blueberries (Lishui Town, Nanjing, China) [53 (link)] and stored at −18 °C at the Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences. High-performance liquid chromatography (HPLC) solvents potassium chloride and acetonitrile were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and TEDIA (Fairfield, OH, USA), respectively. Ferritin (from horse spleen), MOPS buffer, 2,2-bipyridine, and bovine serum protein (BSA) were procured from Sigma-Aldrich (Shanghai, China). The bicinchoninic acid (BCA) and protein-assay kits were purchased from Beijing Solarbio Company (Beijing, China). The reagents used in the experiment are all of analytical grade.