3000 human telomerase immortalized gingival keratinocytes (TIGK, ATCC, Manassas, VA)/well were seeded in a 96-well plate in a culture medium (DermaLife K Medium Complete Kit, Lifeline Cell Technology) without the use of antibiotics. Two hours later, 10 mM stock solutions of CAN, CAS and AgNO3 filtered through a 0.450 μM filter were used to deliver concentrations ranging from 125–1000 μM in the wells containing the cells were incubated for further 24 hours. Cells were cultured in an incubator at 37°C in 5% CO2 environment. Cells without the addition of any metal ions was used as the baseline control. Each treatment condition had three replicates. A cell proliferation assay was performed using CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI) per manufacturer’s instructions. OD490 was read using a spectrophotometer (SPECTRAmax Plus, Molecular Devices, San Jose, CA). A similar human cell proliferation assay utilizing the same metal containing solutions was carried out with primary human gingival fibroblasts (HGF, ATCC, Manassas, VA) cultured in a DMEM (ThermoFisher, Scientific, Catalog No. 11965) with 10% FBS.
Dermalife k medium complete kit
Manufactured by Lifeline Cell Technology
The DermaLife K Medium Complete Kit is a laboratory product designed for the maintenance and culture of keratinocytes, the predominant cell type in the outer layer of the skin. The kit contains all the necessary components, including a specialized medium and supplements, to support the growth and proliferation of keratinocytes in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Celltiter 96 aqueous non radioactive cell proliferation assay kit, by Promega (1 mentions)
Hepatocyte growth factor (hgf), by American Type Culture Collection (1 mentions)
Spectramax plus, by Molecular Devices (1 mentions)
H1 hesc, by WiCell (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
2 protocols using dermalife k medium complete kit
1
Gingival Cell Proliferation Assay
2
Stem Cell Derivation and Culture Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ESC and iPSC cells were cultured on mitomycin C-treated murine embryonic fibroblasts (MEFs, derived from E14.5 CF-1 embryos) in ES cell media consisting of DMEM F-12 supplemented with 1X non-essential amino acids, 1X penicillin-streptomycin, 1X L-glutamine (all from Mediatech), 20% knock serum replacement (Invitrogen), 2-ME (Sigma) and 5 ng/ml bFGF (Invitrogen). Human primary keratinocytes were cultured in DermaLife K Medium Complete Kit (LifeLine Cell Technology). OP9 cells were purchased from ATCC and grown in α-MEM medium with 20% FBS and penicillin-streptomycin. OP9-DL4 cells were established by transducing OP9 cells with lenti-hDL4-mcherry virus. The human embryonic stem cell line H1 (hESC H1) was obtained from the WiCell Research Institute. Induced pluripotent stem cell lines were derived from primary skin cells obtained from skin biopsies as described above under ‘Human iPSCs reprogramming and characterization’.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!