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6 protocols using potassium hexacyanoferrate

1

Multimodal Characterization of Materials

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TEM and HRTEM investigations were performed using a 200 kV American FEI Tecnai G2F20 fitted with field emission gun. XRD analyses were performed with a Empyrean Panalytical diffractometer (Cu Kα with λ = 0.154 nm).
Raman spectroscopy experiments were performed with an Andor SR-500i with a wavelength of 532 nm, acquisition time of approximately 100 seconds per sample-area. X-ray photoelectron spectroscopy (XPS) analyses were performed with an Escalab 250Xi (see ESI).
Electrochemical impedance spectroscopy measurements were performed on a CHI660A workstation (Chenhua Instrument Co. Ltd., China) in a conventional three-electrode system. A modified electrode, a saturated calomel electrode (SCE) and platinum wire were employed as the working, reference and counter electrode, respectively. EIS measurements were performed in 5 mmol L−1 K3[Fe(CN)6]/K4[Fe(CN)6] aqueous solution with 0.1 mol L−1 KCl as the supporting electrolyte in the frequency range from 100 MHz to 100 kHz.
Potassium-hexacyanoferrate(ii)trihydrate (K4[Fe(CN)6]·3H2O), Potassium-hexacyanoferrate(iii) (K3[Fe(CN)6]) and potassium chloride (KCl) were provided by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). UV-Vis absorption was recorded by using a Hanon i5 UV-Vis Spectrophotometer (Jinan Hanon Instruments Co., Ltd., China).
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2

Quantifying Carbonyl Compounds in Dairy Products

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MGO, acetic acid (HPLC grade) and HMF were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide and 3-DG were supplied by TRC (Toronto, Canada). d3-Acrylamide was supplied by Cambridge Isotope Labs (Cambridge, MA, USA), while o-phenylenediamine (OPD) was obtained from J & K Scientific (Beijing, China). Potassium hexacyanoferrate and zinc acetate were supplied by Sinopharm (Beijing, China). Water (HPLC grade) was prepared using a Milli-Q Integral purification system (Molsheim, France). Acetonitrile (HPLC grade) was supplied by Merck (Darmstadt, Germany). Skimmed milk powder (Fonterra, Taranaki, New Zealand) was purchased from the local supermarket. Lactobacillus casei 01 was supplied by Chr. Hansen (Beijing, China). Oasis HLB solid phase extraction (SPE) cartridges (6 mL, 200 mg) were supplied by Waters (Milford, MA, USA).
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3

Electrochemical Sensor Fabrication Using PAN

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Polyacrylonitrile (PAN, average molecular weight = 82,000) powder, N,N-dimethylformamide (DMF), methanol, 2-methylimidazole (C4H6N2, mIM), cobalt nitrate hexahydrate (Co(NO3)2·6H2O), potassium ferricyanide (K3[Fe(CN)6]), potassium hexacyanoferrate (K4[Fe(CN)6]·3H2O), NaOH, hydrogen peroxide (H2O2), dopamine (DA), ascorbic acid (AA), and urea (UA) were all purchased from the Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). All chemicals were of analytical grade and used without further purification. Nafion (5% w/w) was obtained from E. I. Du Pont Company (Wilmington, NC, USA). Besides, 0.1 M NaOH solution was used as a supporting electrolyte. All aqueous solutions were prepared with deionized water (DIW).
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4

Colorimetric Enzyme Activity Assay

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PFTP and GOD (lyophilized powder, 100–250 units per mg) were obtained from MilliporeSigma. Cupric nitrate (Cu(NO3)2·3H2O), glucose, OPD, H2O2, acetic acid (HAC), sodium acetate (NaAC), maltose (Mal), fructose (Fru), sucrose (Suc), lactose (Lac), NaCl, zinc acetate, potassium hexacyanoferrate, and ethanol were obtained from Sinopharm Chemical Reagents. All chemical reagents were used as received without further purification.
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5

Synthesis and Application of PDMDAAC and NaCS

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Poly(dimethyldiallylammonium chloride) (PDMDAAC) was supplied by Aldrich Co., Ltd (Shanghai, China). Cellulose sulfate sodium salt was purchased from J&K SCIENTIFIC LTD (Shanghai, China). Borax and sodium nitrite were purchased from Hunan General Chemical Reagent Works (Changsha, China). Potassium ferricyanide, potassium hexacyanoferrate, sodium dihydrogen phosphate and disodium hydrogen phosphate were all bought from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). All the chemicals were of analytical reagent grade and were used as received without any further purification. Both PDMDAAC and NaCS were also synthesized in our experiments (details can be seen in the Supporting Information).
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6

Synthesis of Isotope-Labeled Lactose Nanoparticles

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Lactose monohydrate (99.0%), melamine (99.0%), ammonium hydroxide (28–30%), tetraethyl orthosilicate (TEOS, 96%), ethanol absolute (99.7%), silver nitrate (99.5%), sodium hydroxide (96%), sodium borohydride (99%), potassium hexacyanoferrate (99.5%), zinc sulfate (99.5%), and Whatman No. 1 filter papers were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd (Beijing, China). [UL-13C6glc]-Lactose monohydrate (the isotope contained six 13C) was purchased from Cambridge Isotope Laboratories (CIL, USA). Polyvinylpyrrolidone (PVP, MW 40 kDa) and bovine serum albumin (BSA) were purchased from Sigma, USA. All aqueous solutions were prepared using deionized water (18.2 MΩ cm, Milli-Q, Millipore, GmbH) throughout all experiments.
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