EdU was determined using the BeyoClick EdU-555 cell proliferation test kit (Beyotime, China). HCT116 and LoVo cells were transfected with miRNA mimics and inhibitors, respectively. Negative controls were inoculated into 24-well culture plates. Cells were cultured in media containing 10% FBS for 48 h and then fixed with 4% paraformaldehyde. Cells were incubated in 50 mM EdU solution for 2 h before staining with DAPI. Cells were stained using Hoechst solution, and their DNA content was visualized using a fluorescence microscope.
Beyoclick edu 555 cell proliferation test kit
Manufactured by Beyotime
Sourced in China
The BeyoClick EdU-555 cell proliferation test kit is a laboratory equipment product designed for the detection and quantification of cell proliferation. The kit utilizes the incorporation of the thymidine analogue EdU (5-ethynyl-2'-deoxyuridine) into newly synthesized DNA, providing a reliable method to measure cell division.
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2 protocols using beyoclick edu 555 cell proliferation test kit
1
Cell Proliferation Assay using EdU
2
Quantifying Cell Proliferation with EdU-555 Kit
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Using the BeyoClick™ EdU-555 Cell proliferation test kit (Beyotime, China) to confirm the cell proliferation. The cells of the different treatment groups were placed in 20 mmol/L EdU for 2 h. Then, the cells were immobilized at room temperature with 4% paraformaldehyde for 15 min. After scoured with PBS, the cells were permeated with 0.3% Triton X-100, then washed with PBS and placed in Click reaction solution at 25°C in the dark for 30 min. The cells were dyed with Hoechst 33,342 for 10 min and observed with an AX-70 fluorescent microscopy (Leica Microsystems Inc.).
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