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14 protocols using dimethylformamide dmf

1

Fabrication of PVDF-HFP/PI Composite Membranes

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Polyvinylidene fluoride hexafluoropropylene (PVDF-HFP, Mw = 400,000) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Polyimide (PI) resin was supplied by the Hangzhou Plastic UNITA Special Technology Co., Ltd. (Hangzhou, China). Dimethylformamide (DMF) and acetone were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All chemical reagents were used as received without further purification.
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2

Synthesis of Gold Nanoparticles

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Sodium silicate solution (≥27 wt% SiO2), 3-aminopropyltrimethoxysilane (APTS, 97%), and chloroauric acid (≥99.9%) were obtained from Sigma-Aldrich, Alfa Aesar, and Jiuyue Chemical Ltd Co. (Shanghai, China), respectively. Sodium citrate, hydrochloric acid, mercuric nitrate (Hg(NO3)2), phosphate buffer saline (PBS), ethanol, and dimethyl formamide (DMF), all of analytical grade, were purchased from Sinopharm Chemical Reagent Ltd Co. (Shanghai, China). DMEM (Dulbecco's Modified Eagle Medium) were purchased from Sangon Biotech Reagent Ltd Co. (Shanghai, China). Other materials were obtained from native suppliers (Changsha Longhe Chemical and Glass Experimental Products Ltd Co. (Changsha, China)), and all materials were used as received without further purification.
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3

Multifunctional Hyaluronic Acid Hydrogel

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Hyaluronic acid sodium salt (HA) from bovine vitreous humor was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Gallic acid (GA), adipic acid dihydrazide (ADH), tyramine hydrochloride (Tyr), galactose oxidase (GalOx, ≥ 30 units/mg, solid), horseradish peroxidase (HRP, ≥ 160 units/mg, solid), 2-(N-morpholino)ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), N-hydroxy succinimide (NHS) and DCFH-DA (2,7-dichlorodihydrofluorescein diacetate) were obtained from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Dimethylformamide (DMF) was obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Evans blue dye, dialysis membrane (molecular cutoff ​= ​3500 ​Da), malondialdehyde (MDA), glutathione (GSH), alkaline phosphatase (ALP), aspartate transaminase (AST) and glutamic-pyruvic transaminase (GPT) assay kits were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). IL-4, IL-6 and p-Nrf2 antibodies were purchased from Affinity Biosciences (Cincinnati, OH, USA), the remaining antibodies were sourced from Proteintech Group Inc. (Wuhan, China).
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4

Synthesis of β-Cyclodextrin Derivatives

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β-cyclodextrin was purchased from Aladdin Co. Ltd. (Shanghai,China). 2-butenoic acid (99%, HPLC grade), dimethylformamide (DMF, 99%, HPLC grade), N, N’-Carbonyldiimidazole (CDI, AR grade) and 4-Dimethylaminopyridine (DMAP, AR grade) were from Sinopharm Co. Ltd (Shanghai, China). Methyl butenate was from Sigma (St. Louis, MO, U.S.A). All other reagents were of analytical grade and used as received.
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5

Boronic Acid-Functionalized PLGA Microgels for Insulin Delivery

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Poly(lactic-co-glycolic acid) (PLGA; D,L-lactide/glycolide 50/50; Mw 12 kDa) was purchased from the Jinan Daigang Biomaterial Co., Ltd (Jinan, China). Poly(vinyl alcohol) (PVA-1788, medical grade) was obtained from Xi’an Tianzheng Medicinal Materials Co., Ltd (Xian, China). 3-Acrylamido phenylboronic acid (AAPBA) was sourced from the Beijing Pure Chemical Co., Ltd. (Beijing, China), N-vinylcaprolactam (NVCL) was purchased from Hubei Jusheng Co., Ltd. (Wuhan, China) and dimethyl formamide (DMF) and 2,2-azobisisobutyronitrile (AIBN) from the Sinopharm Chemical Reagent Co. (Shanghai, China). Insulin was obtained from Sigma-Aldrich (San Francisco, CA). Dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH) and phosphate buffered saline (PBS) were purchased from the China National Medicines Corporation Ltd. (Beijing, China). All solvents used in this work were of analytical grade.
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6

Gelatin-Based Biosensor Fabrication

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Gelatin
(type A from porcine
skin, less than 300 bloom), HRP (type VI, salt-free, 250–330
units/mg solid), 3-(4-hydroxy-phenyl) propionic acid (HPA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide
(EDC), N-hydroxysuccinimide (NHS), GalOx, and d-galactose were purchased from Sigma-Aldrich (St. Louis, MO).
Dimethylformamide (DMF) was obtained from Sinopharm Chemical Reagent
Co., Ltd (Shanghai, China). The chemical reagents were used as obtained
without purification. The dialysis membrane (molecular cutoff = 3500
Da) was purchased from Spectrum Laboratories (Rancho Dominguez, CA).
Mouse bone mesenchymal stem cells (BMSC) were obtained from the Cell
Bank, Type Culture Collection, Chinese Academy of Sciences, Shanghai,
China.
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7

Synthesis and Characterization of Metal-Organic Framework

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Unless otherwise specified, all chemicals and reagents were of reagent grade and used without further purification. Copper(ii) nitrate trihydrate (Cu(NO3)2·3H2O) was obtained from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). DBP (purity > 99.5% (GC)), 1,3,5-benzenetricarboxylic acid (BTC) were purchased from Aladdin chemistry Co., Ltd (Shanghai, China). Sodium persulfate (Na2S2O8, 99.0%) and all other chemicals such as dimethyl formamide (DMF), sodium hydroxide (NaOH) and sulfuric acid (H2SO4) were provided by Sinopharm Chemical Reagent Co., Ltd (Beijing, China).
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8

Peptide Synthesis and Analytical Reagents

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Fluoren-9-ylmethoxycarbonyl (Fmoc) amino acid derivatives with side-chain protections were purchased from the GL Biochem Co., Ltd. (Shanghai, China). 2-Nitrophenyl β-d-galactopyranoside (ONPG), oxidized nicotinamide adenine dinucleotide (NAD+), 6-phosphogluconate, iodonitrotetrazolium chloride (INT) and reagents for peptide synthesis were purchased from the Aladdin Industrial Corporation (Shanghai, China). Regents about ethylenediaminetetraacetic acid (EDTA), sodium deoxycholate, hexadecyltrimethylammonium bromide (CTAB), beta-mercaptoethanol, sodium salts, and dimethylformamide (DMF) were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Muller-Hinton Broth (MHB) was purchased from the Beijing Ao Bo Xing Bio-Tech (Beijing, China). Agar powder and Tris were purchased from Gene Company Co., Ltd. (Hong Kong, China). ZoRBAX SB-C18 PreHT Column was purchased from the Agilent Technologies Inc. (Santa Clara, CA, USA). Acetonitrile with HPLC grade was purchased from the Thermo Fisher Scientific Inc. (Shanghai, China). Bovine Serum Albumin (BSA) was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China).
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9

Synthesis and Evaluation of Novel Apocynin Derivatives

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All chemicals used were commercially available. Apocynin (D1, 98% purity) was purchased from Sigma Chemicals Co. (St. Louis, MO, USA). K2CO3,CHCl3, benzyl bromide, dimethylformamide (DMF), CH3CN, NaHCO3, Br2, aniline para-fluoroaniline, 2,4-dimethoxyaniline, 2,4-difluoroaniline, and 4-chloroaniline were purchased from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). Proton nuclear magnetic resonance (1H NMR) and carbon nuclear magnetic resonance (13C NMR) spectra were recorded at on a Bruker Avance III 500MHz NMR Spectrometer (Bruker, Switzerland). Chemical shifts (δ) and coupling constants (J) were expressed in ppm and Hz, respectively.
Human glioma U87 cells, U251 cells, and rat C6 glioma cells were purchased from Chinese Academy of Medical Sciences (Beijing, China). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and trypsin were purchased from invitrogen (Carlsbad, CA, USA). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) and phosphate-buffered saline (PBS) were purchased from Gibco BRL Co., Ltd. (Grand Island, New York, USA). Cells were cultured in DMEM supplemented with 10% FBS in a 37°C incubator. Cells were washed with PBS and harvested by trypsinization.
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10

Thermoplastic Polyurethane and Polyimide Composite

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Thermoplastic polyurethanes (TPU) was purchased from BASF (Rhineland-Palatinate, Germany). Polyimide (PI, Mw = 80,000) was from Hangzhou Plastic UNITA special Technology Co., Ltd. (Hangzhou, China). Dimethylformamide (DMF) were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Fluorescein (Mw = 332.31) was from Tianjin Dingshengxin Chemical Co., Ltd. (Tianjin, China). Rhodamine B was from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). PE separator with a thickness of 20 μm was purchased from Celgard (Charlotte, NC, USA). All reagents were originally used without further purification.
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