Anti his rabbit mab

Anti-KSHV vIL-6 rabbit polyclonal antibody (PAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA). Anti-KSHV ORF65 mouse monoclonal antibody (MAb) was used to detect KSHV capsids [68 (link)]. Anti-KSHV RTA peptide antibodies were generated by immunization of rabbits with RTA peptide as previously described [69 (link)]. Anti-phospho-AKT (Ser473) mouse MAb, anti-AKT rabbit PAb, anti-GRK2 rabbit PAb, anti-CD31 mouse MAb and anti-His rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-CXCR2 rabbit PAb, anti-α-tubulin and GAPDH mouse MAbs, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-smooth muscle actin (SMA) rabbit PAb were obtained from AbbiotecTM (San Diego, CA, USA). 12-O-tetradecanoylphorbol-13-acetate (TPA; 20 ng/mL), doxycycline hyclate (1 μg/ml) and sodium butyrate (1 mM) were obtained from Sigma (St. Louis, MO, USA). 4′, 6′-diamidino-2-phenylindole (DAPI) was purchased from Beyotime Institute of Biotechnology (Nantong, China).

Anti-KSHV LANA ORF73 rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA) [78 (link)]. Anti-phospho-AKT (Ser473) rabbit MAb, anti-AKT rabbit polyclonal antibody (PAb), anti-Flag M2 rabbit MAb, anti-His rabbit MAb and anti-HA rabbit PAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-GRK2 mouse MAb, anti-CXCR2 rabbit PAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-GFP mouse MAb was from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western blotting analysis was performed as described previously [83 (link)]. MK-2206, an AKT inhibitor, was purchased from Selleck Chemicals (Shanghai, China).