Ammonium bicarbonate, dithiothreitol (DTT), and iodoacetamide (IAA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MS grade trypsin protease, Spectra™ Multicolor Broad Range Protein Ladder (10 to 260 kDa, catalog number: 26634), and HPLC grade solvents were purchased from Thermo Scientific™ Pierce™ (Rockford, IL, USA). Acetonitrile (HPLC grade), LiChrospher® WP 300 C18 RP-HPLC column, and Millipore ZipTip® C18 Pipette Tips were obtained from Merck (Kenilworth, NJ, USA). Other chemicals and solvents used were of analytical grade and purchased from Sigma-Aldrich (USA).
Spectra multicolor broad range protein ladder 10 to 260 kda
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Spectra™ Multicolor Broad Range Protein Ladder (10 to 260 kDa) is a molecular weight marker designed for estimating the molecular weights of proteins in polyacrylamide gel electrophoresis (PAGE) and western blotting applications. The ladder consists of 10 pre-stained protein bands with molecular weights ranging from 10 to 260 kDa.
Automatically generated - may contain errors
2 protocols using spectra multicolor broad range protein ladder 10 to 260 kda
1
Proteomic Sample Preparation Protocol
2
Proteomics Sample Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ammonium bicarbonate, dithiothreitol (DTT) and iodoacetamide were purchased from Sigma-Aldrich (USA). Mass-spectrometry grade trypsin protease, Spectra™ Multicolor Broad Range Protein Ladder (10 to 260 kDa), and HPLC grade solvents used in the studies were purchased from Thermo Scientific™ Pierce™ (USA). Millipore ZipTip® C18 Pipette Tips were obtained from Merck, USA. Other chemicals and reagents of analytical grade were purchased from Sigma-Aldrich (USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!